Spectroscopic data for components of the Reference Dye Sampler Kit—Table 23.4

Component	Solvent	Abs (nm) *	Em (nm) *	QY †
Quinine sulfate	Water	347 ‡	455 ‡	0.55 ‡
Fluorescein	Water	490 §	514 §	0.92 §
5-CTMR **	Methanol	542	568	0.68
Sulforhodamine 101	Ethanol	578	605	0.90
Nile blue	Ethanol	636	665	0.27
* Approximate absorbance (Abs) and fluorescence emission (Em) maxima. † Fluorescence quantum yield at 22°C. ‡ Standard values in 0.5 M H₂SO₄. § Standard values in 0.1 M NaOH. ** CTMR = Carboxytetramethylrhodamine.

For Research Use Only. Not for use in diagnostic procedures.

Spectroscopic Data for Components of the Reference Dye Sampler Kit—Table 23.4