Qubit® Technical Resources
Find technical resources for your Qubit® 2.0 Fluorometer including a variety of application and technical notes such as Qubit® Quantitation Platform vs. the Nanodrop® Spectrophotometer and Testing for Bias in the Quant-iT™ RNA Assay. Also available are Qubit® frequently asked questions, citations, and valuable customer ratings & reviews.

Featured application

Qualifying DNA preparations for next-generation sequencing (NGS)
Prior to committing the effort and resources required for next-generation sequencing (NGS), it’s important for researchers to be able to quantify and assess the quality of the DNA starting material. In a 2013 publication, Simbolo et al. reported a reliable and cost-effective method to qualify various DNA preparations for NGS, including those derived from frozen tissue and FFPE samples. Of the instruments they tested, their data showed that DNA quantification results obtained using the Qubit® Fluorometer were highly reproducible and were consistent with qPCR data for DNA quantity—even for partially degraded DNA from FFPE samples.

Technical Notes

Qubit® Quantitation Platform vs. Spectrophotometer

Qubit® Fluorometer Vs. Nanodrop™ Spectrophotometer

Detection and quantitation of nucleic acids are vital to many biological studies. Historically, DNA and RNA have been quantified using spectrophotometry to measure absorbance at 260 nm. Although this method is most commonly used, it can be unreliable and inaccurate.



Testing for Bias in the Qubit® RNA Assay

Testing for Bias in the Quant-iT™ RNA Assay
The Qubit® RNA assay measures RNA concentration in samples containing 250 pg/μL to 100 ng/μL of RNA. To test for the presence and extent of any bias in the Qubit® RNA assay for any of the bases (A, C, G, or U), or for single- or double-stranded RNA, the response of single- and double-stranded RNA homopolymers in the Qubit® RNA assay has been examined.



Single-stranded DNA in the Qubit® dsDNA Assays for the Qubit® Fluorometer

Single-stranded DNA in the Quant-iT™ dsDNA Assays for the Qubit® Fluorometer
Invitrogen offers two assays for quantifying double-stranded DNA (dsDNA) using the Qubit® Fluorometer: the Qubit® dsDNA HS (for High Sensitivity) Assay and the Qubit® dsDNA BR (for Broad Range) Assay. One advantage of these assays is their specificity for dsDNA. This study was carried out to investigate whether the Qubit® dsDNA quantitation assays for the Qubit® Fluorometer accurately determine the concentration of dsDNA when the samples contain short or long single-stranded DNA (ssDNA).



The Use of Glycogen and GlycoBlue™ Reagent in the Qubit® DNA and RNA Assays as Measured on the Qubit® 2.0 Fluorometer

The Use of Glycogen and GlycoBlue Reagent in the Qubit DNA and RNA Assays as Measured on the Qubit 2.0 Fluorometer
Glycogen (a branched-chain carbohydrate) and GlycoBlue™ reagent (glycogen covalently linked to a blue dye) are reagents commonly used to facilitate nucleic acid precipitation without adding extraneous nucleic acids to the sample. This study was carried out to determine whether glycogen or GlycoBlue™ reagent affects the accuracy of nucleic acid quantitation using Qubit® kits for the Qubit® 2.0 Fluorometer.

Qubit® Fluorometer Quick Resources

Literature Citations
See how your colleagues are using the Qubit® 2.0 Fluorometer with our library of references and citations.

FAQs
Get the answers to some Frequently Asked Questions about the Qubit® 2.0 Fluorometer.

Ratings & Reviews
See what your peers are saying about the Qubit® Fluorometer by reading customer feedback.