Qubit® Fluorometer Ratings & Reviews

“For production we need to make a large mass of DNA, and we need to measure the concentration accurately before aliquoting.  Our production methodology yields large amounts of RNA along with the DNA.  So we treat the sample with RNaseA, but even after treatment the individual RNA monomers still absorb at 260 nm, so we can’t use a spectrophotometer to quantify the DNA.  This is where the Qubit comes in.  We can use it to accurately quantify the DNA in the presence of the digested RNA since the dye only binds to DNA.  We looked at the measurement relative to the intensity of known standards on a gel and the Qubit appears to be quite accurate.”
Mark G. Wise, Ph.D.
Staff Scientist, Bacterial Barcodes, Inc. (a wholly-owned subsidiary of BioMerieux, Inc.)

“It gives me the possibility to measure very diluted samples. Good, quick and easy.”
Silvia Rodriguez, Institut de Recerca Biomedica de Barcelona (IRB)

“Based on the Qubit measurements, Nanodrop overestimated the amount of RNA in the blood spot samples about 10 times and this number would agree with the amount of cDNA and qPCR numbers that we are getting from the samples.”
Julia Busik, Asst. Professor, Michigan State University

“If we compare the concentration from the Qubit, the Nanodrop and the Bioanalyzer, we would always use the Qubit quantitation."
Sue Sipkovsky, Lab Manager, Center for Animal Functional Genomics, Michigan State University

“The Quant-iT Protein Assay completely saved my experiment. The UV spec inaccurately quantified my protein.”
Bryan Warf Ph.D. candidate University of Oregon

“It saves an hour a day in quantitating samples.”
Steve Enkemann Director of Microarray Core Facility Moffitt Cancer Center

“I trust my determinations of concentrations of DNA, RNA and proteins better.”

“Faster quantification of DNA, faster sequencing.”

“It's made it easier to measure protein concentration.”

“Better accuracy in quantitation of DNA or RNA yields more reproducible results”

“It has enabled me to quantify my samples much quicker than before”

“Yes, it has changed my workflow because it is more accuracy and sensitivity than UV spec. I so happy with Qubit. It's my new son.”

“It has enabled me to quantify my samples much quicker than before”

“Yes, I can analyze my samples quickly and trust the results more completely.”

“For cost and ease of use, Qubit is hard to beat.”

“It is faster than other quantitation methods.”

“Yes because I have been able to really quantify my RNA and Proteins. RNA was especially important because we are collecting from a very low cell number.” “I can measure just DNA.”

“I am more sure about amount of sample I have.”

“Cloning speed and efficiency increased dramatically, and qRT-PCR is more precise.”

“I have more confidence in DNA quantitation results.”

“The Qubit has made it easier to quantitate DNA after extraction so we can more accurately estimate the amount we extracted.”

“Using our UV spectrophotometer was a lot of extra work, so I dreaded measuring my samples. The Qubit is easy and accurate.”

“We use it for routine PCR. It affected in time saving and accuracy.”

“We are more confident that in the accuracy of our quantitations. People perform quantitations more often rather than estimating.”

“Trouble shooting qPCR is easier when starting material is properly characterized, and results from different experimental conditions can be confidently compared knowing that the starting amount of template was exactly the same. Also helps first strand cDNA reactions to perform optimally. The same general idea goes for DNA and protein as well.”

“I no longer worry about losing sample to measuring how much there is.” “I am more confident about my downstream design & results.”

“More reliable than spec -more confidence in results.”

“It makes the process of work a lot more efficient.”

“I trust my determinations of concentrations of DNA, RNA and proteins better.”

“Faster quantification of DNA, faster sequencing.”

“It's made it easier to measure protein concentration.”

“Better accuracy in quantitation of DNA or RNA yields more reproducible results”

“It has enabled me to quantify my samples much quicker than before”

“Yes, it has changed my workflow because it is more accuracy and sensitivity than UV spec. I so happy with Qubit. It's my new son.”

“It has enabled me to quantify my samples much quicker than before”

“Yes, I can analyze my samples quickly and trust the results more completely.”

“For cost and ease of use, Qubit is hard to beat.”

“It is faster than other quantitation methods.”

“Yes because I have been able to really quantify my RNA and Proteins. RNA was especially important because we are collecting from a very low cell number.” “I can measure just DNA.”

“I am more sure about amount of sample I have.”

“Cloning speed and efficiency increased dramatically, and qRT-PCR is more precise.”

“I have more confidence in DNA quantitation results.”

“The Qubit has made it easier to quantitate DNA after extraction so we can more accurately estimate the amount we extracted.”

“Using our UV spectrophotometer was a lot of extra work, so I dreaded measuring my samples. The Qubit is easy and accurate.”

“We use it for routine PCR. It affected in time saving and accuracy.”

“We are more confident that in the accuracy of our quantitations. People perform quantitations more often rather than estimating.”

“Trouble shooting qPCR is easier when starting material is properly characterized, and results from different experimental conditions can be confidently compared knowing that the starting amount of template was exactly the same. Also helps first strand cDNA reactions to perform optimally. The same general idea goes for DNA and protein as well.”

“I no longer worry about losing sample to measuring how much there is.” “I am more confident about my downstream design & results.”

“More reliable than spec -more confidence in results.”

“It makes the process of work a lot more efficient.”

“It gives me greater confidence in my qPCR results.”

“I have more material for downstream applications, even when the DNA/RNA has a low concentration.”

“Yes, I'm able to do more biological replicates in my protein work because my protein quantitation is so much faster and more reliable.”

“It saves a lot of time, especially with protein quantification.”

“It is faster then other quantitation methods.”

“More reliable quantification of low concentrations of nucleic acids. Improved reproducibility of downstream qPCR experiments”

“I have more confidence in my PCR results.”

“More confidence in quantitation”

“The big change is less time workflow. The quantification time is less, and the PCRs are OK at the first shot.”

“It's faster and I trust the quantitation more”

“Now we know exactly the concentrations of our standards in qRT-PCR, and discovered that our 'assumed' concentrations according to UV-VIS of the concentrate and calculated concentrations of the dilutions were way off and inconsistent.”

“We don't have access to a UV/Vis spectrophotometer, making DNA quantitation difficult. BQ (Before Qubit), my colleagues were estimating DNA concentration based on agarose gels. The Qubit has saved us significant time and allowed for more accuracy in downstream DNA applications.”