GeneArt™ Subcloning & Express Cloning Service

Using an alternative production procedure with pre-prepared expression vectors, GeneArt™ can clone your synthesized genes directly into selected expression plasmids, saving 4-5 business days compared to the standard workflow for expression ready constructs with cloning into pMx series vector first.

This Express cloning option is different from the still available classical subcloning service after gene synthesis (see table below). As deliverable, you will receive expression ready genes - your synthesized gene in the selected expression vector, no additional copy in a pMx cloning vector will be provided.

How to order

• Order online via the online portal and add the express cloning service to your gene synthesis order
• To order by email, fill out the submission form and submit form to geneartsupport@lifetech.com

Turnaround time for expression ready genes starts at 11 business days (depending on the length of your gene). Adding the gene synthesis express upgrade cuts additional 2 days, so it is possible to realize a turnaround time of 9 business days for your cloned, expression ready gene (see table below).

Genes qualifying for Express cloning must be <5kb and not complex (optimization of your sequence can reduce complexity).

The following vectors are currently available for Express Cloning and can be directly selected during ordering (more vectors will be included for Express cloning over time):

Figure 1: Express cloning directly into an expression vector

Figure 2: Classical subcloning from pMx into an expression vector

*Expression vector example. Multiple choices for vector available.

We have substantial experience using Gateway® recombination cloning technology, and the GeneArt® Subcloning Service can be used to move your synthetic gene into any vector you choose, including novel vectors built using the GeneArt® Plasmid Construction Service. Focus on your results and leave the subcloning to GeneArt® Services.

• Competitive price
• Convenient—tell us what you need and receive your ready-to-use clone quickly
• Reliable—vector integrity is verified by restriction digestion and inserts are 100% sequence-verified and documented
• Confidential—no data or material are provided to third parties
• Extendable—get more of your plasmid DNA (any amount) if needed

Choose “Subcloning (SC)” when setting up your project online.

The basic subcloning service includes subcloning your DNA insert into the vector you specify. You’ll receive:

• 5 µg of plasmid DNA containing your insert
• 100% sequence verification