Biochemical Kinase Assays | Thermo Fisher Scientific

Thermo Fisher Scientific offers you the broadest set of biochemical fluorescent assays to address high-throughput screening, hit confirmation and lead optimization programs. All Invitrogen kinase assay technologies are homogenous, non-radioactive, and highly miniaturizable – and are available as kits, custom or bulk reagents.

For quality kinase profiling data on rapid timelines, choose SelectScreen, the industry’s most utilized kinase profiling service.

To compile the components needed to make the right biochemical kinase assay for your research, use our new Drug Discovery Assay Maker web tool.

See the LanthaScreen Kinase Activity Assay Reactivity Table

Request assay information Use assay maker

Choose the right biochemical HTS kinase assay for your research?

	Compatible with fluorescently labeled whole protein substrates	Ideal for kinetic studies including on/off rates or for kinases with no known substrate	Validated specifically for lipid kinases; can use with unmodified peptide or lipid substrates	Primary assay for SelectScreen Kinase Profiling Service
	Order now	Order now	Order now	Order now
	LanthaScreen Activity Assay	LanthaScreen Eu Kinase Binding Assay	Adapta Assay	Z'-LYTE Assay
Application Technology basis	Phospho-antibody TR-FRET	Tracer-based TR-FRET	ADP detection by TR-FRET	FRET peptide phosphorylation
Application	Primary screening	Unactivated or low-activity kinase target screening	Primary and secondary screening	Secondary screening, hit validation, and profiling
Number of kinases validated	200	over 320	20	233
Readout	Endpoint	Endpoint or Kinetic	Endpoint	Endpoint
Total assay volume	20 µL	15 µL	15 µL	20 µL
ATP concentration range	Tested up to 1 mM	ATP not used	Tested from 10 to 100 µM	Tested up to 1 mM
Substrate type	Peptide or protein	No substrate needed	Peptide, protein or lipid	Peptide
Total assay volume	Substrate concentration range	100 nM–1 µM	Flexible; must be greater than ATP concentration	2 µM only
Plate signal stability	Up to 18 hours	Up to 24 hours	Up to 18 hours	4–18 hours (kit-dependent)
Cell lysate compatible		✓
Overcoming interference	Time-resolved readout allows read after interfering signals have decayed	Time-resolved readout allows read after interfering signals have decayed; use of epitope-tag ensures no contaminating kinase is detected	Time-resolved readout allows read after interfering signals have decayed; red Alexa Fluor dye as acceptor further enhances ability to overcome interference	Preread at 445/520 nm
Cost per well	$–$$	$–$$	$$–$$$	$$
Total assay volume		✓	✓	✓

Optimize with multiple high-throughput assay formats

Thermo Fisher Scientific offers four high-throughput assay technologies for your biochemical kinase research.

LanthaScreen Eu Kinase Binding Assays—Obtain sensitive detection of kinase inhibitors, including allosterics; interrogate active and non-activated kinases; track slow-binding inhibitors in real time, and compare inhibitor on-off rates with this robust assay technology.
LanthaScreen Kinase Activity Assays—A powerful toolbox for TR-FRET kinase activity assay development – choose exactly the reagents you need and acquire high quality data with minimal assay interference.
Adapta Universal Kinase Assays—A universal TR-FRET assay suitable for all kinases; including difficult-to-assay targets such as lipid kinases.
Z´-LYTE Kinase Assay—An ideal assay for secondary screening, hit validation and profiling, Z’-LYTE is the primary assay technology utilized in our SelectScreen Kinase Profiling Service.

Lower Throughput Assay Technologies

NDP Sensor—A universal, real-time kinetic kinase assay platform at a very low price per well

Resources

Support

Drug Discovery & Development Support Center

Find tips, troubleshooting help, and resources for support related to your drug discovery experiments.

For Research Use Only. Not for use in diagnostic procedures.