Custom Rabbit Polyclonal Antibody Production Protocols

• Custom rabbit antibodies—three standard protocol options for immunizing rabbits, collecting serum, testing bleeds, reporting titer results, and delivering antisera
• Rabbit titer guarantee—if you allow us to oversee design and production of the peptide and/or protein antigen used for injection, we guarantee to reach a minimum rabbit antiserum titer of 1:50,000 as measured with our ELISA assay
• Full-service integration—combine this immunization service with our complete set of services for antigen preparation and antibody purification (see Custom Polyclonal Antibodies pages for details)
   

Of the three standard options for rabbit polyclonal antibody production, this protocol is optimized to produce antiserum in the shortest possible time. It is the most affordable option, popular among individual academic researchers.

The default immunization schedule uses two NZW SPF rabbits (New Zealand White rabbits that are Specific Pathogen-Free). Injections are subcutaneous (SQ) as emulsions in Complete Freund's Adjuvant (CFA) or Incomplete Freund's Adjuvant (IFA); alternative adjuvants can be used if requested.

Antigen preparation, such as peptide synthesis and/or immunogen conjugation, occurs before Day 0. Protocol days are approximate (± 2 days).

Of the three standard options for rabbit polyclonal antibody production, this protocol is optimized to maximize speed, titer, and volume for a competitive price. Originally developed and adopted by the NIH, this procedure meets the minimum requirements mandated by most pharmaceutical companies.

The default immunization schedule uses two NZW SPF rabbits (New Zealand White rabbits that are Specific Pathogen-Free). Injections are subcutaneous (SQ) as emulsions in Complete Freund's Adjuvant (CFA) or Incomplete Freund's Adjuvant (IFA); alternative adjuvants can be used if requested.

Antigen preparation, such as peptide synthesis and/or immunogen conjugation, occurs before day 0. Protocol days are approximate (± 2 days).

Of the three standard options for rabbit polyclonal antibody production, this protocol is optimized to maximize titer and volume by ensuring that sufficient time is provided for full maturation of the secondary immune response and serum antibody production in each rabbit. Based on the method originally developed by Baines and Korn (1990), this procedure exceeds the minimum requirements mandated by most pharmaceutical companies.

The default immunization schedule uses two NZW SPF rabbits (New Zealand White rabbits that are Specific Pathogen-Free). Injections are subcutaneous (SQ) as emulsions in Complete Freund's Adjuvant (CFA) or Incomplete Freund's Adjuvant (IFA); alternative adjuvants can be used if requested.

Antigen preparation, such as peptide synthesis and/or immunogen conjugation, occurs before Day 0. Protocol days are approximate (± 2 days).

Custom rabbit antibodies manufactured by the Seller are guaranteed to reach a minimum titer of 1:50,000 by indirect ELISA assay for at least one rabbit so long as the following conditions are met: (a) Seller designed and produced the peptides and/or proteins used for immunization; (b) the design criteria did not contain Buyer mandated limitations, including but not limited to, cross reactivity requirements, limited protein sequence region, and/or post-translationally modified or cleaved peptides; and (c) the antibodies were generated in rabbits. If the antibody does not reach the minimum titer and all the above criteria are met, Seller will either (y) boost one animal (minimal) at no charge or (z) alter the carrier or antigen to boost one animal (minimal) for up to two months free of charge. Once one animal reaches the minimum titer of 1:50,000, the guarantee will be met. If a 1:50,000 titer cannot be achieved, the Buyer will be provided one additional custom antibody to be ordered free of charge with a re-design of the peptide and/or protein used for immunization. All ancillary services requested by the Buyer on the new project such as purification and extensions will be charged a la carte at the discretion of the Seller.

Upon completion of any antibody protocol, Buyers are provided seven business days to evaluate the antibody quality and provide instructions on the requested animal disposition. If no instructions are received from the Buyer, animals will be placed on hold and begin incurring daily maintenance fees according to the current fee schedule. If more than 30 days pass without receipt of instructions, animals will be scheduled for termination. An invoice for accrued charges will be sent to Buyer for all fees incurred. Payment of such fees is required prior to provision of additional services.