CellInsight CX7 LED Pro HCS Platform

LED-based fluorescence as well as brightfield illumination and confocal capabilities

Catalog numbers: HCSDCX7LEDPRO

 

The Thermo Scientific CellInsight CX7 LED Pro High-Content Screening Platform is an LED-based platform that offers a choice of imaging modes to extract the information you need from your samples. Both well-by-well and channel-by-channel, you can select the right modes to read your sample—with the resolution and dynamic range that results from the high performance of the optical train and sensitive camera. Use the entire fluorescence spectrum to optimize your assay and select either widefield or confocal optics for any channel. Browse the features, specifications, videos, sample data, and reagent selection guide sections.

Features

In addition to the common features of all high-content screening platforms, the CellInsight CX7 LED Pro platform offers:

Next generation sCMOS camera

The CX7 LED Pro has been upgraded with a best-in-class sCMOS camera, offering greater than 95% peak quantum efficiency and low background 1.0e-ready noise. This near-perfect quantum efficiency further accelerates your high-throughput performance thanks to reduced exposure times across all wavelengths. Boast your experiment’s assay window, with up to 65,536 shades of gray detection while not sacrificing background noise thanks to the -20˚C cooling.

 

Introducing Olympus X-line objectives to the CX7 LED Pro

The CX7 LED Pro now offers Olympus X-line objectives to further improve the instruments imaging quality and assay performance. Publication quality imaging is now standard using the new CX7 LED Pro platform.

 

Live-cell experiment optimization

The CX7 LED Pro light has been optimized for live-cell experiments. Laser illumination provides flatter and brighter images with low background.

 

Confocal imaging mode

CrEST™ spinning-disk confocal technology with dual 40 or 70 micron pinhole size. The CrEST confocal is engineered directly into the optical path to provide high-resolution confocal imaging capabilities.

Brightfield imaging mode

Using an LED array for RGB and amber illumination, this mode allows users to make colorimetric absorbance measurements of histology samples with classic stains like H&E.

 

Widefield imaging mode

The 7-color light engine reduces switching times and intensity fluctuations to reduce scan times and boost quantitative performance.

 

Automation

The CX7 LED Pro is configured for fully automated plate handling and scanning.

 

Multiplexing

Multiplex your colorimetric absorbance data with fluorescence measurements, offering more possibilities for verification and correlation.

 

Specifications

Category

Attribute

Description

Optics

Camera

Photometrics High-Resolution Fluorescent Camera:

  • High-resolution, thermoelectrically cooled to –20°C
  • >95% peak quantum efficiency (QE)
  • Low background 1.0e-ready noise
  • 16-bit 65,536 shades of grey detection

 

Light source

LED, solid-state 7-color light engine used with provided filter sets offers the following excitation/emission capabilities (Ex/Em, in nm):

  • 386/440 (e.g., Alexa Fluor 350 dye)
  • 438/480 (e.g., Alexa Fluor 405 dye)
  • 485/521 (e.g., Alexa Fluor 488 dye)
  • 549/600 (e.g., Alexa Fluor 555 dye)
  • 560/607 (e.g., Alexa Fluor 594 dye)
  • 650/694 (e.g., Alexa Fluor 647 dye)
  • 740/810 (e.g., Alexa Fluor 750 dye)

 

Objectives

Standard (Olympus™ objectives):

  • 10x (0.4 NA) X-line
  • 20x (0.45 NA) Achroplan system
  • 20x (0.7 NA) Olympus objectives
  • 40x (0.6 NA) Olympus objectives

Optional (Olympus objectives):

  • 1.25x (0.04NA)
  • 2x (0.08 NA)
  • 4x (0.16 NA) X-Line
  • 20x (0.8 NA) X-Line
  • 40x (0.95 NA) X-Line
  • 60x (0.90 NA)

 

Image compatibility

JPG, BMP, GIF, PNG, TIF, C01, DIB

Physical characteristics

Dimensions

20 in x 32 in x 18 in (50.8 cm x 81.3 cm x 45.7 cm)

 

Weight

68 kg (150 lb)

System

Data management

Compatible with Thermo Scientific Store Image and Database Management Software

 

Software

Thermo Scientific HCS Studio 5.0 Cell Analysis Software with Cell-Painting Bioapplication

 

PC

Windows 10 Professional 64-Bit PC with 32GB (4x8GB) RAM, Intel® Xeon® Processor 3.7GHz Turbo, RAID I Controller with 1.8TB Hard Drive and 256 Boot Drive.

Includes Dell 24" High Resolution Widescreen Monitor or equivalent, keyboard and mouse

 

Wattage

300 W

Videos and demos

Sample data

HeLa cells imaged using the CellInsight CX7 platform

 

HeLa cell image acquired using the Thermo Scientific CellInsight CX7 platform and stained with Invitrogen HCS NuclearMask Blue (blue), Invitrogen MitoTracker Orange (green), and Invitrogen Alexa Fluor 647 Phalloidin (red).

A549 cells imaged using the CellInsight CX7 platform

 

A549 cell image acquired using the Thermo Scientific CellInsight CX7 platform and stained with Invitrogen HCS CellMask Blue (blue), Invitrogen Alexa Fluor 488 Phalloidin (green), and Invitrogen Alexa Fluor 750 goat anti–mouse IgG secondary antibody (pink).

Human breast cancer tissue section imaged using the CellInsight CX7 platform

 

Three-color brightfield image of fixed human breast cancer tissue stained with KI67-DAB and counterstained with hematoxylin and eosin and imaged with the Thermo Scientific CellInsight CX7 platform.

HeLa cells imaged using the CellInsight CX7 LED instrument

 

HeLa cells labeled with tubulin antibody followed by Invitrogen Alexa Fluor Plus 488 antibody and Hoechst 34580 staining. Imaging was performed on the CellInsight CX7 LED instrument using a 60x objective and confocal mode.

HeLa cells imaged using the CellInsight CX7 LED instrument

 

HeLa cells labeled with tubulin antibody followed by Invitrogen Alexa Fluor Plus 647 antibody and Hoechst 34580 staining. Imaging was performed on the CellInsight CX7 LED instrument using a 60x objective and confocal mode.

HeLa cells imaged using the CellInsight CX7 LED instrument

 

HeLa cells labeled with ATP synthase V antibody followed by Alexa Fluor Plus 488 antibody and Hoechst 34580 staining. Imaging was performed on the CellInsight CX7 LED instrument using a 60x objective and confocal mode.

Reagent selection guide

The Thermo Scientific CellInsight CX7 LED Pro high-content instrument allows you to take advantage of the entire fluorescence spectrum to optimize your assay—and multiplex your components to ask more in-depth biological questions. Learn more about reagents for cell viability, proliferation, and function or reagents to determine cell structure. For more specific applications using assays and high-content platforms, check out Assay Selection.

For Research Use Only. Not for use in diagnostic procedures.