Dye-labeled phosphoramidites are intended for oligonucleotide synthesis designed for molecular assays, microscopy, and microarrays. A broad range of labeling options are available with coupling efficiency, emission, and absorption spectra comparable to other commercially available dyes.

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Designed for oligonucleotide synthesis of diagnostic research and manufacturing development

These phosphoramidites may be used on automated DNA synthesizers following standard synthesis and deprotection procedures. Fluorescent tags can be incorporated at the 5′ end of an oligonucleotide.

Technical specifications for DyLight-labeled phosphoramidites

Thermo Scientific DyLight (DY) fluorescent labels offer choices with spectrally distinct colors for oligonucleotide labeling with ß-cyanoethyl phosphoramidites (CEP). Oligo labeling with ß-cyanoethyl phosphoramidites (CEP). Available to use in research applications without the constraints of licensing and royalty fees.

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 DY547 CEPDY647 CEPDY677 CEP
Cat. No.SY6332SY6334SY6336
FormulaC38H54IN4O2PC47H63N4O5PSC55H67N4O5PS
Molecular weight756.74 g/mol827.07 g/mol927.18 g/mol
HPLC purity≥90% at 547 nm≥85% at 647 nm≥85% at 677 nm
IdentityUV-Vis spectrum conforms to that of the authentic sample
Shipping / storage conditionDry ice / –20°C
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Standard dye-labeled phosphoramidites technical specifications

Phosphoramidites with fluoresceine and its derivatives like 6-carboxyfluorescein (6-FAM), hexachlorofluorescein (HEX), tetrachlorofluorescein (TET) are also available to provide you with flexibility in oligo labeling.

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 Fluorescein CEP6-FAM CEPHEX CEPTET CEP
Cat. No.27-160627-1608SY6342SY6341
Chemical name[(3′,6′-dipivaloylfluoresceinyl)-5,6-carboamidohexyl]-1-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite

Fluorescein dipivalate phosphoramidite, 6-isomer

6-(4,7,2′,4′,5′,7′-hexachloro-3′,6′-dipivaloylfluoresceinyl-6-carboxamido)-hexyl-1-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite5′-tetrachloro-fluorescein phosphoramidite
FormulaC46H58N3O10P C46H58N3O10PC46H52N3O10Cl6PC46H54N3O10Cl4P
Molecular weight843.95 g/mol843.94 g/mol1050.63 g/mol981.73 g/mol
CAS / PubChem#

N/A

204697-37-0 / 58114954

1360547-55-2877049-90-6
HPLC purity≥90%≥90%≥95%≥95%
31P NMR purity85%≥95% (6-isomer)N/AN/A
Shipping / storage conditionDry ice / –20°C
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Resources

Phosphoramidite Learning Resource

Access webinars, videos, articles, and more to optimize your success in oligonucleotide synthesis for diagnostic and therapeutic manufacturing process development.

mRNA Production Learning Library

Browse the educational assets related to raw materials selection, process development using in vitro transcription, scale-up synthesis, and the latest trends in mRNA production.

For Research Use Only. Not for use in diagnostic procedures.

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