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Protein Purification Support
Explore the “Getting Started” and “Troubleshooting” sections for solutions to top inquiries and common problems. Browse through the “Guides and Tools” section to access comprehensive product-related support resources.
Getting Started
- How does affinity chromatography work?
- I see that you offer multiple immobilization resins for protein immobilization. Which one should I use?
- What is hybrid purification and when should it be used?
- What reagents do you recommend to prevent nonspecific binding besides detergents when using the ProBond™ System?
- What are fusion tags?
Troubleshooting
- I am using the ProBond™ Purification System and my protein is not eluting. What should I do?
- My native eluted fractions do not contain the His-tagged protein when using the ProBond™ System. Do you have any suggestions for me to try?
- I’m getting very low protein expression from after isolation using the ProBond™ System. Can you provide any suggestions?
- I am using the NativePure™ Affinity Purification System. Some of the recombinant protein is in the flow through and wash fractions. What should I do?
- My tag does not cleave. What do I do?
Guides and Tools
General
- Guide: Avidin-Biotin Technical Handbook
- Guide: Fusion Protein Purification Resins
- Guide: Protein Purification Handbook
- Guide: Antibody Production and Purification Handbook
- Tech Tip: Protein Stability and Storage
Protein Immobilization
- Tech Tip: Quantitate Immobilized Protein
- Tech Tip: Prepare Molecules with Poor Solubility for Immobilization on Affinity Supports
- Tech Tip: Pack Beaded Affinity Resin into Columns
- Tech Tip: Prepare a Reversible and Measurable Sulfhydryl-reactive Affinity Column
- Tech Tip: Degas Buffers for Use in Affinity and Gel Filtration Columns
- Tech Tip: Attach a Protein onto Glass, Silica, or Quartz Surface Using a Cleavable Crosslinker
- Tech Tip: Attach a Protein onto a Gold Surface
- Tech Tip: Attach an Antibody onto Glass, Silica or Quartz Surface
Affinity Purification
- Tech Tip: Ion Exchange Chromatography
- Tech Tip: Batch and Spin Cup Methods for Affinity Purification of Proteins
- Tech Tip: Spin Cup and Column Dimensions and Volumes
- Tech Tip: Optimize Elution Conditions for Immunoaffinity Purification
- Tech Tip: Remove Air Bubbles from Columns to Restore Flow Rate
- Tech Tip: Binding Characteristics of Antibody-binding Proteins: Protein A, Protein G, Protein A/G and Protein L
Antibody Purification
Search our database for Product Documents: Manuals, Product Literature, Citations and References
Search our database for Quality and Safety Documents: SDS, COA
General
- Overview: Protein Purification
- Overview: Protein Purification Accessories
- Learning Center: Protein Biology
- Protein Biology Resource Library (useful technical, application, and other learning resources including application notes and methods)
- Guide: Protein Handbook
- Selection: Automated Magnetic Separations for Proteomics
- Video: Protein Analysis Tips and Tricks
Protein Immobilization
- Overview: Protein Immobilization
Affinity Purification
- Overview: Protein Purification Using Affinity Chromatography
- Overview: Chromatography and Protein Purification
- Overview: Biotin Affinity Purification
- Protocol: Using CaptureSelect Affinity Products
- Selection: Biotinylation Reagent Selection Tool
Recombinant Protein Purification
- Overview: Recombinant Protein Purification
- Protocol: Purification of Polyhistidine-Containing Recombinant Proteins with Ni-NTA Purification System
Protein Enrichment
- Overview: Protein Enrichment
- Selection: Protein Enrichment Kit Selection Guide
- DailyCalcs Science Calculator: An app designed to calculate molarity, dilution, molecular weight, and more. Also includes a transfection calculator you can use to simplify transfection experimental setup.
Custom Services
Custom Chromatography Services
When your specific process needs cannot be met with products available to order, our custom chromatography services are available to build you a unique solution. We can design a custom affinity ligand or resin to suit your needs, or provide custom column packing services. And of course our custom services include regulatory support documentation, as well as world-class application support.
Protein Expression and Production Services
Have us utilize our extensive suite of expression vectors and host systems to deliver the necessary protein yield for your application. We offer protein expression and production services in baculovirus and mammalian host systems, as well as protein purification and analysis services.
GeneArt™ Cell Lines and Proteins
GeneArt™ Cell Lines and Proteins are a suite of service offerings designed to help facilitate protein expression in mammalian cell lines. We can help with every step of the process, including gene design, synthesis, cloning, protein production, and even stable cell generation. Alternatively, we can help with just the parts of the process you request.
For Research Use Only. Not for use in diagnostic procedures.