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Direct electron detector camera for cryo-EM
The Thermo Scientific Falcon 4i Direct Electron Detector is the ideal solution for your single particle analysis and cryo-tomography applications. With its unique combination of high image quality, high throughput, efficient lossless data compression enabled via electron-event representation (EER), and a streamlined solution for data management and quality monitoring, the Falcon 4i Detector offers a productivity and performance boost that meets the demands of today’s scientific and industrial communities.
The Falcon 4i Detector is fully integrated in Thermo Scientific EPU and Tomography Software, allowing for smooth daily instrument operation and data acquisition. The camera’s output is recorded through the Data Management Platform (DMP), which also facilitates the organization, viewing, and sharing of single particle cryo-EM data. You can easily view all the project data and metadata at the microscope or remotely through a secure connection, and can comment and share it with collaborators.
Note: The Falcon 4i Direct Electron Detector is fully compatible with 200 and 300 kV Thermo Scientific Krios, Glacios, and Talos Cryo-Transmission Electron Microscopes (Cryo-TEMs) operating with Windows 10. A Falcon 4i Upgrade Kit is available for the Falcon 4 Direct Electron Detector. The Falcon 4i Upgrade Kit upgrade is also applicable for the Thermo Scientific Selectris and Selectris X Imaging Filters.
Thermo Scientific Falcon 4i Direct Electron Detector
Selectris and Selectris X Imaging Filters
Designed for stability and speed, the Thermo Scientific Selectris and Selectris X Imaging Filters are post-column imaging filters that improve the contrast of TEM images, resulting in high-resolution structures up to atomic resolution. Paired with the latest generation Thermo Scientific Falcon 4i Direct Electron Detector, Selectris Filters enable you to obtain high-resolution structures quickly, revealing new biological insights. To further increase the productivity and ease-of-use of your cryo-electron microscopy (cryo-EM) research, these filters are fully integrated into Thermo Scientific EPU Software, Thermo Scientific Tomography Software, and instrument operation software. The Selectris and Selectris X Imaging Filters are available on Thermo Scientific Krios and Glacios Cryo-TEMs.
The Falcon 4i Direct Electron Detector enables high resolution and throughput.
Apoferritin reconstruction at 1.2 Å resolution created from 3,600 images of 297k particles acquired in 6 hours (EPU data acquisition on the Thermo Scientific Krios G4 Cryo-TEM). Recorded at a dose rate of 5.4 e/p/s.
Amyloid-β 42 filaments isolated from human brain resulting in a 2.5 Å resolution reconstruction. Data collected at dose rate 8 e/p/s, 40 e/A2 total dose and 570 movies/hr. Collaboration with MRC Laboratory of Molecular Biology. doi: https://doi.org/10.1101/2021.10.19.464936
Unsurpassed image quality through higher DQE
Get leading detective quantum efficiency (DQE) across the full spatial frequency range. High DQE at low spatial frequency lets you visualize and align smaller and more flexible proteins, while high DQE at high spatial frequencies lets you achieve results with fewer images or increased resolution.
Large pixels with high signal-to-noise ratio (SNR)
The large signal generated by the Falcon 4i Detector’s large pixels, combined with low noise, yields high SNR – electron events can be unambiguously separated from the background noise (left) in comparison to detectors with smaller pixels (right).
Higher throughput for more images per hour
The increased internal frame rate, in combination with improved overhead, yields a significant increase in throughput, depending on the use case.
Lossless data compression with electron-event representation (EER)
Electron-event representation (EER) data enables efficient cryo-EM file storage with full preservation of spatial and temporal resolution. Counted events of all raw frames are available for processing with full temporal resolution (320 fps) and spatial resolution (events are localized to one-sixteenth of a pixel). This super resolution capability maximizes the benefit of the Falcon 4i Detector’s superior DQE at high spatial frequencies.
Style Sheet for Komodo Tabs
재품 표 사항에 대한 스타일시트
| Camera architecture | Direct electron detection | |
| Sensor size | 4,096 × 4,096 pixels, ~ 5.7 x 5.7 cm2 | |
| Pixel size | 14 x 14 μm2 | |
| TEM Operating voltage | 200 kV, 300 kV | |
| Internal frame rate | 320 fps | |
| Frame rate to storage | 320 fps (EER mode) | |
| Camera Overhead time | 0.5 s per acquisition | |
| File formats | EER (native), MRC, TIFF, LZW TIFF | |
| Lifetime (<10% DQE degradation) | 5 years in normal use (1.5Ge/px) | |
| Detection Modes | Electron counting mode Survey mode (fast linear mode) | |
| Imaging performance in EER mode (4k x 4k) | 300 kV | 200 kV |
| DQE (0) | 0.92 | 0.91 |
| DQE (½ Nq) | 0.72 | 0.62 |
| DQE (1 Nq) | 0.50 | 0.33 |
To find out more, contact us today by filling out the form below.
This 1.2 Å resolution reconstruction of apoferritin was created from 3,600 images of 297k particles acquired in 6 hours (EPU data acquisition on the Thermo Scientific Krios G4 Cryo-TEM). Recorded at a dose rate of 5.4 e/p/s.
Cryo-EM structures of Amyloid-β 42 filaments isolated from human brain. Data was collected at a flux of 8 e/p/s; magnification of 0.74 A/pix; 40 e/A² total dose and a throughout of 570 movies/hr resulting into a 2.5 Å reconstruction. (Data collected in collaboration with MRC – Laboratory of Molecular Biology, Cambridge) doi: https://doi.org/10.1101/2021.10.19.464936
3D SPA reconstruction of the Homopentameric GABAA receptor at 2.1Å resolution. Images are acquired on a Krios Cryo-TEM with the Falcon 4 and EER and processed with Relion 3. Image courtesy of: Radu Aricescu, MRC-LMB Cambridge, UK and Abhay Kotecha, Thermo Fisher Scientific, Eindhoven, Netherlands.
Falcon 4i in 4 minutes
This 1.2 Å resolution reconstruction of apoferritin was created from 3,600 images of 297k particles acquired in 6 hours (EPU data acquisition on the Thermo Scientific Krios G4 Cryo-TEM). Recorded at a dose rate of 5.4 e/p/s.
Cryo-EM structures of Amyloid-β 42 filaments isolated from human brain. Data was collected at a flux of 8 e/p/s; magnification of 0.74 A/pix; 40 e/A² total dose and a throughout of 570 movies/hr resulting into a 2.5 Å reconstruction. (Data collected in collaboration with MRC – Laboratory of Molecular Biology, Cambridge) doi: https://doi.org/10.1101/2021.10.19.464936
3D SPA reconstruction of the Homopentameric GABAA receptor at 2.1Å resolution. Images are acquired on a Krios Cryo-TEM with the Falcon 4 and EER and processed with Relion 3. Image courtesy of: Radu Aricescu, MRC-LMB Cambridge, UK and Abhay Kotecha, Thermo Fisher Scientific, Eindhoven, Netherlands.
Falcon 4i in 4 minutes
전염병 연구
Cryo-EM 기술은 3D 생물학적 구조를 거의 원형(native) 상태에서 다양한 스케일로 관찰할 수 있어, 신속하고 효율적인 치료제 개발에 도움을 줍니다.
신약 발견
동급 최강의 약물 개발을 위해 더 많은 주요 약물 표적 클래스에 대한 합리적인 약물 설계를 활용하는 방법을 알아 보십시오.
단일 입자 분석
단일 입자 분석(SPA)은 거의 원자 분해능 수준에서 구조의 규명과 동적 생물학적 프로세스 및 생물학적 분자 복합체/조립체의 구조 규명을 가능하게 하는 초저온 전자 현미경 기술입니다.
초저온 단층촬영(Cryo-Tomography)
초저온 전자 단층촬영(cryo-ET)은 개별 단백질에 대한 구조적 정보 뿐만 아니라 세포내 공간 정렬에 대한 정보를 제공합니다. 이것은 진정으로 독창적인 기술이며 왜 이 방법이 세포 생물학에 엄청난 잠재력을 가지고 있는지를 설명해 줍니다. Cryo-ET는 광학 현미경과 단일 입자 분석 같은 거의 원자 분해능 수준의 기술 사이의 간극을 해소할 수 있습니다.
통합 구조 생물학
단백질 기능에 대해 이해하려면, 개별 단백질 이상의 복잡한 구조 정보 를 알아야 합니다. 통합적인 구조 생물학은 질량 분석법과 cryo EM 을 결합하여 대규모 동적 복합 구조에 대해 파악합니다.
단일 입자 분석
단일 입자 분석(SPA)은 거의 원자 분해능 수준에서 구조의 규명과 동적 생물학적 프로세스 및 생물학적 분자 복합체/조립체의 구조 규명을 가능하게 하는 초저온 전자 현미경 기술입니다.
초저온 단층촬영(Cryo-Tomography)
초저온 전자 단층촬영(cryo-ET)은 개별 단백질에 대한 구조적 정보 뿐만 아니라 세포내 공간 정렬에 대한 정보를 제공합니다. 이것은 진정으로 독창적인 기술이며 왜 이 방법이 세포 생물학에 엄청난 잠재력을 가지고 있는지를 설명해 줍니다. Cryo-ET는 광학 현미경과 단일 입자 분석 같은 거의 원자 분해능 수준의 기술 사이의 간극을 해소할 수 있습니다.
통합 구조 생물학
단백질 기능에 대해 이해하려면, 개별 단백질 이상의 복잡한 구조 정보 를 알아야 합니다. 통합적인 구조 생물학은 질량 분석법과 cryo EM 을 결합하여 대규모 동적 복합 구조에 대해 파악합니다.
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