Sample Preparation and Plasma Protein Depletion

Single-step, single use protein depletion products for the removal of high-abundance human serum proteins from serum, plasma or CSF, before mass spec and/or gel analysis for biomarker discovery, validation, or diagnostics.

  • Cost-effective, single use sample preparation
  • Minimize cross contamination between samples
  • Up to 99% protein removal from samples
  • Mono specificity helps ensure reproducible results
SDS-PAGE analysis of the CaptureSelect™ HumanPlasma14 depletion of abundant proteins from human plasma. 
M: P-6649 molecular marker
LOAD: 100x diluted human plasma
FT: plasma flow-through
S: stripping of bound plasma fraction. 
The protein fractions were stained with Sypro ruby dye.

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Products:

Product Target
CaptureSelect™ HumanPlasma14Depletes the top 14 most abundant proteins from human blood plasma: albumin, IgG, IgM, IgA, IgE, IgD and free light chains, transferrin, fibrinogen, α-1 antitrypsin, apolipoprotein A1, α-2 macroglobulin, α-1 acid glycoprotein, and haptoglobin; enables >99% depletion of albumin and other high-abundance proteins, with no nonspecific binding and the highest reported loading capacity
CaptureSelect™ HumanPlasma AlbuminDepletes albumin from human plasma or serum
CaptureSelect™ HumanPlasma Ig/AlbuminDepletes human plasma or serum of albumin and all Igs, including IgG, IgA, IgM, IgD, IgE, and free light chains
CaptureSelect™ Multi Species AlbuminDepletes albumin from plasma or serum derived from a number of different species (e.g., mouse, rat, rabbit, goat, bovine, guinea pig, human, and horse)

Plasma Sample Prep Literature:

CaptureSelect™ HumanPlasma Ig/Albumin is a premium offering over other products available as it, besides IgG’s also removes other Ig’s and all free antibody light chains.Development of a Chip/Chip/SRM platform using digital chip isoelectric focusing and LC-Chip mass spectrometry for enrichment and quantitation of low abundance protein biomarkers in human plasma.Performance Evaluation of Novel Affinity Ligands for Immunodepletion.

 

For more information on CaptureSelect™ products and services please contact us.

CaptureSelect™ information