Human Monocyte Cell Isolation Kits | Thermo Fisher Scientific

Which human monocyte cell isolation kit is right for you?

	Highest purity for molecular assays	Affordable & pure, flow-compatible cells	Untouched—most physiological relevant cells
	Dynabeads CD14	Dynabeads FlowComp Human CD14	Dynabeads Untouched Human Monocytes
Isolation method	Depletion or positive isolation	Positive isolation	Negative isolation
Starting samples	Any sample (whole blood, MNC, bone marrow)	Any sample (whole blood, MNC, bone marrow)	MNC*
# MNC processed (mL whole blood processed)	1-2 x 10⁹ MNC 200 mL whole blood	1-2 x 10⁹ MNC 80 mL whole blood	1 x 10⁹ cells
Purity	Highest 98–100%	Higher 95–99%	High 90–95%
Yield	Highest 95–100%	Standard 45–60%	Standard 50–70%
Viability	>95%	>95%	>95%
Isolation time	~30 min	~60 min	~50 min
Isolation temp.	Cold	Cold	Cold
Applications	Depletion of unwanted monocytes or positive isolation for molecular isolation of proteins & nucleic acids	Cellular assays (cell culture, cell activation/proliferation, cytokine studies) Molecular isolation of proteins & nucleic acids	Cellular applications for physiological studies (functional studies, flow analysis, study of resting/activated cells)
Bead-free target cells	No	Yes (but antibody present)	Yes
Compatible with flow cytometry/sorting	No	Yes	Yes
Shelf life from production date	3 years	2 years	2 years
Required materials	Magnets	Magnets	Magnets
	Order now	Order now	Order now

Learn more about our monocyte isolation kits

Dynabeads CD14

Dynabeads CD14 are superparamagnetic beads coupled with an anti-human CD14 antibody that enable easy isolation or depletion of human CD14+ cells directly from any sample, including whole blood, bone marrow, buffy coat, mononuclear cells (MNC), and tissue digests. Advantages of Dynabeads CD14:

Fast and efficient isolation of CD14+ cells from any sample—no columns required
High purity, yield, and viability of positively isolated CD14+ cells for molecular downstream assays
Fast and efficient depletion of CD14+ cells from any sample

Dynabeads FlowComp Human CD14 Kit

Dynabeads FlowComp Human CD14 contain magnetic beads that provide a robust and versatile tool to positively isolate pure CD14+ monocytes from whole blood, bone marrow, buffy coat, mononuclear cells (MNC), or tissue digests. The cells can be released from the beads using the FlowComp Release Buffer.

Rapid isolation of CD14+ monocytes from any sample—no columns required
Pure and bead-free CD14+ monocytes for any downstream application

Dynabeads Untouched Human Monocytes Kit

The Dynabeads Untouched Human Monocytes Kit is used to isolate pure and viable monocytes from PBMC by negative isolation. The kit depletes T cells, B cells, NK cells, dendritic cells, granulocytes, and erythrocytes, while the negatively isolated human monocytes are left in the sample. The isolated monocytes cells are in perfect shape for any functional assay and application. Advantages of the Dynabeads Untouched Human Monocytes Kit:

High purity, recovery, and viability of human monocytes
Easy to use and to scale up
No columns required

THIS IS FOR HUMAN RESEARCH—NOT FOR MOUSE