DEACTIVATING-ReadyPouch™ Agarose Gels | Thermo Fisher Scientific

이제 간단히 파우치 상태 그대로 입구를 열어 가열하고, stain dye를 추가한 후 gel trainer에 부으면 agarose gel을 어느 누구보다 손쉽게 만들 수 있게 됩니다.

ReadyPouch™ 는 더이상 무거운 용기에 담긴agarose 용액을 들거나, 옮길 필요가 없고, TAE buffer와 agarose powder를 섞어주지 않고도, 다양한 목적에 사용 가능한 UltraPure™ grade의 Premixed type의 1% agarose gel 용액 입니다.

무료 샘플 신청하기

ReadyPouch agarose video - 4 simple steps

Traditional agarose: A tale of too many steps

What researchers are saying

"I don't know why anyone would want to make their gel from scratch."

"One less step. Just grab-n-go."

ReadyPouch™ Agarose Gel benefits

No boil-overs
Wide resolution range
High gel strength
Ready-to-use agarose (1%) premixed in TAE
Fewer bubbles
No detectable DNase or RNase activity

ReadyPouch™ Agarose Gel applications

PCR fragments >0.5 kb
Southern blotting
Analytical separation of DNA fragments in 0.5–23 kb range

ReadyPouch™ Agarose Gels—4 easy steps

Vent

ReadyPouch™ Agarose Gel workflow—Vent

VENT by tearing pouch along the dotted line

Heat

ReadyPouch™ Agarose Gel workflow—Heat

HEAT in microwave on HIGH for 90 seconds

Add

ReadyPouch™ Agarose Gel workflow—Add stain

ADD 2 drops of stain

Pour

ReadyPouch™ Agarose Gel workflow—Pour

POUR into casting tray

일반 1% agarose gel과 ReadyPouch™ agarose gel을 이용한 전기영동의 결과 비교

A 1% Agarose Gel

B ReadyPouch™ Agarose Gel

Lambda DNA was digested with various restriction enzymes for 1 hour, and 250 ng of each digest was then loaded on a standard 1% agarose gel (A) and a ReadyPouch™ Agarose Gel (B). The gels were run for 30 min at 85 V and imaged on an E-Gel® Imager for 0.5 sec. Lanes 1 and 14: 250 ng of 1 Kb Plus DNA Ladder. Lane 2: 2 µL Low Mass DNA Ladder. Lanes 3 and 4: AgeI digest. Lanes 5 and 6: AseI digest. Lanes 7 and 8: BamHI digest. Lanes 9 and 10: PvuII digest. Lanes 11 and 12: HindIII digest. Lane 13: BamHI/HindIII double digest.

ReadyPouch™ Agarose Gels—DEACTIVATING THIS ENTIRE PAGE