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DNA Ladders for Agarose Gel Electrophoresis
Invitrogen DNA ladders are available for a wide variety of size ranges, applications, and formats. Optimized size ranges are available for easy analysis of large and small fragments. These DNA ladders are stable during prolonged incubation at room temperature as well as after multiple freeze-thaw cycles, and include loading dyes for clear tracking and analysis.
Benefits of Invitrogen DNA ladders
Precise sizing
Each DNA ladder fragment is purified individually using proprietary chromatography-based technology, resulting in DNA ladders of exceptional quality that exceed industry standards. The chromatography-based purification method yields exceptionally pure DNA fragments.
Reliable quantification
The concentration of each DNA fragment in the DNA ladders is determined spectrophotometrically, which enables precise in-gel quantification. In addition, our chromatography-based purification method does not use organic solvents, which makes these DNA ladders environment-friendly products.
Reduced environmental footprint
These DNA ladders are shipped at ambient temperature in non-insulated packages, which aids in reducing the annual carbon footprint associated with packaging and shipping. Products shipped at ambient temperatures have been tested rigorously to ensure product quality and stability.
The Invitrogen family of DNA ladders include the following:
- Invitrogen E-Gel DNA Ladders are formulated specifically for maximum performance on Invitrogen E-Gel precast agarose gels and are provided in ready-to-load formats
- Invitrogen TrackIt DNA Ladders are designed for use with agarose gels and are provided in convenient, ready-to-load formats
- Invitrogen Mass DNA Ladders are designed for accurate quantification and sizing of DNA fragments in agarose gels
- Invitrogen DNA Ladders are provided with the loading buffer in a separate tube
DNA ladder selection guide
The key to accurate band analysis is to use the correct DNA ladder suited for your particular application. This DNA ladder selection guide helps you choose the DNA ladder appropriate for your application.
| Product | Range, base pair (bp) | Ready to load (in loading buffer) | Tracking dyes |
|---|---|---|---|
| E-Gel DNA Ladders | 10 to 15,000 | Yes | Xylene Cyanol, Tartrazine (1X E-Gel Sample Loading Dye) or Xylene Cyanol, Orange G (1X E-Gel Sample Loading Dye) |
| TrackIt DNA Ladders | 1,000 to 10,000 (high mass) 100 to 2,000 (low mass) | Yes | TrackIt Cyan/Yellow Loading dye OR TrackIt Cyan/Orange Loading Dye |
| Mass DNA Ladders | 10 to 15,000 | No | Provided with 10X BlueJuice Gel Loading Buffer |
| DNA Ladders | 10 to 15,000 | No | Provided with 10X BlueJuice Gel Loading Buffer |
Invitrogen DNA ladders: product offerings
DNA Ladders for E-Gels
Invitrogen E-Gel DNA ladders are premixed with loading buffer and designed specifically for use with the E-Gel precast agarose gels in sizes ranging from 10 bp to 15 kb.
E-Gel DNA ladder selection guide
| E-Gel 96 High Range DNA Marker | E-Gel 1 Kb Plus Ladder | E-Gel 1 Kb Plus Express Ladder | E-Gel Low Range Quantitative DNA Ladder | E-Gel 50 bp DNA Ladder | E-Gel Sizing DNA Ladder | E-Gel Ultra Low Range DNA Ladder |
|---|---|---|---|---|---|---|
| 400–10,000 bp | 100–15,000 bp | 100–5,000 bp | 100–2,000 bp | 50–2,500 bp | 50–1,500 bp | 10–300 bp |
 |  |  |  |  |  |  |
TrackIt DNA Ladders
TrackIt DNA Ladders are premixed in loading buffer and ready to use. Available in popular sizes ranging from 10 bp to 12 kb, TrackIt DNA ladders are also supplied with loading dye for sample DNA. TrackIt DNA Ladders have reference bands (indicated in bold in the images below) for easy orientation. Select the ladder of interest below.
TrackIt DNA ladder selection guide
| TrackIt 1 Kb Plus DNA Ladder | TrackIt 100 bp DNA Ladder | TrackIt 50 bp DNA Ladder | TrackIt Ultra Low Range DNA Ladder |
|---|---|---|---|
| 100–15,000 bp | 100–2,000 bp | 50–2,500 bp | 10–300 bp |
 |  |  |  |
DNA ladders and mass ladders
DNA ladders are ideal for precise DNA sizing and approximate quantification. DNA mass ladders consist of equimolar mixtures of DNA fragments and are specifically created for quantitative estimation of DNA mass in gels.
DNA ladder and DNA mass ladder selection guide
| High DNA Mass Ladder | 1 Kb Plus DNA Ladder | 100 bp DNA Ladder | Low DNA Mass Ladder | 50 bp DNA Ladder | Ultra Low Range DNA Ladder |
|---|---|---|---|---|---|
| 1,000–10,000 bp | 100–15,000 bp | 100–2,000 bp | 100–2,000 bp | 50–2,500 bp | 10–300 bp |
 |  |  |  |  |  |
DNA loading buffers and tracking dyes
Loading buffers are solutions used for easy loading and tracking of DNA samples in agarose gels. Tracking dyes serve as visual markers of migration during electrophoresis and indicate when maximum resolution of the DNA fragments has been achieved. Additionally, visualization of DNA bands is not obscured by the tracking dyes because they run outside the limits of most DNA samples.
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