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The Invitrogen E-PAGE High-Throughput (HTP) Precast Gel System is designed for fast, bufferless medium- and high-throughput protein analysis.
The E-PAGE High-Throughput (HTP) Precast Gel System is designed for fast, bufferless medium- and high-throughput protein analysis. Invitrogen E-PAGE Gels are self-contained, precast gels that include a gel matrix and electrodes packaged inside a disposable cassette. E-PAGE Gels are available in 48-well or 96-well formats.
Features of the E-PAGE High-Throughput Precast Gel System include:
- Ease-of-use—Quick setup and fast separations (23 minutes) for 1D protein analysis
- Fast loading—Compatible with multichannel pipettors and robotic loading
- Efficient western blotting and staining—Optimized protocols and reagents
Protein separation on an E-PAGE Gel requires an Invitrogen E-Gel Power Snap Plus Electrophoresis Systems, a compact and automated platform designed for medium- and high-throughput electrophoresis.
E-PAGE Gels
We offer two kinds of E-PAGE Gels:
- Invitrogen E-PAGE 8% Protein Gels, 48-well—Each gel contains 48 sample lanes and 4 marker lanes with a 3.2-cm separation length, and can be loaded with a 12- or 8-channel pipettor or 4- or 8-tip robotic system.
- Invitrogen E-PAGE 6% Protein Gels, 96-well—Each gel contains 96 sample lanes and 8 marker lanes in a patented staggered-well format with a 1.6-cm run length; well openings are compatible with the standard 96-well plate format and can be conveniently loaded with a multichannel pipettor or 8-, 12-, or 96-tip liquid handling robotic device.
Both E-PAGE 48-well and 96-well cassettes are conveniently opened with a butterfly opener to remove the gel for downstream staining or blotting. In addition, each cassette is labeled with a unique barcode to facilitate identification of the gel using commercial barcode readers.
| Available gel sizes | 48-well (3.7 mm thick) 96-well (3.7 mm thick) |
| Storage conditions | Room temperature |
| Shelf life | 6–9 months |
| Available polyacrylamide concentrations | 6% (96 wells), 8% (48 wells) |
| Separation range | 10–200 kDa |
| For use with (equipment) mini gels | E-Base integrated devices |
| Mode of separation | Molecular weight |
| E-PAGE 48-well 8% gels | E-PAGE 96-well 6% gels | |
|---|---|---|
| Cassette size | 13.5 (l) x 10.8 (h) x 0.67 cm (w) | 13.5 (l) x 10.8 (h) x 0.67 cm (w) |
| Gel thickness | 3.7 mm | 3.7 mm |
| Gel volume | 50 mL | 50 mL |
| Well depth | 3 mm | 3 mm |
| Number of wells | 48 + 4 markers | 96 + 8 markers |
| Well format | 2 x 26 | Staggered 8 x 13 |
| Well opening | 3.6 mm x 2.2 mm | 3.8 mm x 1.8 mm |
| Running distance | 32 mm | 16 mm |
| Run time | 23 min | 14 min |
| Space between well centers | 4.5 mm | 9 mm |
| Sample volume | 15 µL | 20 µl |
| Maximum protein load | 20 µg | 20 µg |
Migration pattern of Invitrogen E-PAGE MagicMark Unstained Protein Standard.
The E-PAGE High-Throughput (HTP) Precast Gel System is designed for fast, bufferless medium- and high-throughput protein analysis. Invitrogen E-PAGE Gels are self-contained, precast gels that include a gel matrix and electrodes packaged inside a disposable cassette. E-PAGE Gels are available in 48-well or 96-well formats.
Features of the E-PAGE High-Throughput Precast Gel System include:
- Ease-of-use—Quick setup and fast separations (23 minutes) for 1D protein analysis
- Fast loading—Compatible with multichannel pipettors and robotic loading
- Efficient western blotting and staining—Optimized protocols and reagents
Protein separation on an E-PAGE Gel requires an Invitrogen E-Gel Power Snap Plus Electrophoresis Systems, a compact and automated platform designed for medium- and high-throughput electrophoresis.
E-PAGE Gels
We offer two kinds of E-PAGE Gels:
- Invitrogen E-PAGE 8% Protein Gels, 48-well—Each gel contains 48 sample lanes and 4 marker lanes with a 3.2-cm separation length, and can be loaded with a 12- or 8-channel pipettor or 4- or 8-tip robotic system.
- Invitrogen E-PAGE 6% Protein Gels, 96-well—Each gel contains 96 sample lanes and 8 marker lanes in a patented staggered-well format with a 1.6-cm run length; well openings are compatible with the standard 96-well plate format and can be conveniently loaded with a multichannel pipettor or 8-, 12-, or 96-tip liquid handling robotic device.
Both E-PAGE 48-well and 96-well cassettes are conveniently opened with a butterfly opener to remove the gel for downstream staining or blotting. In addition, each cassette is labeled with a unique barcode to facilitate identification of the gel using commercial barcode readers.
| Available gel sizes | 48-well (3.7 mm thick) 96-well (3.7 mm thick) |
| Storage conditions | Room temperature |
| Shelf life | 6–9 months |
| Available polyacrylamide concentrations | 6% (96 wells), 8% (48 wells) |
| Separation range | 10–200 kDa |
| For use with (equipment) mini gels | E-Base integrated devices |
| Mode of separation | Molecular weight |
| E-PAGE 48-well 8% gels | E-PAGE 96-well 6% gels | |
|---|---|---|
| Cassette size | 13.5 (l) x 10.8 (h) x 0.67 cm (w) | 13.5 (l) x 10.8 (h) x 0.67 cm (w) |
| Gel thickness | 3.7 mm | 3.7 mm |
| Gel volume | 50 mL | 50 mL |
| Well depth | 3 mm | 3 mm |
| Number of wells | 48 + 4 markers | 96 + 8 markers |
| Well format | 2 x 26 | Staggered 8 x 13 |
| Well opening | 3.6 mm x 2.2 mm | 3.8 mm x 1.8 mm |
| Running distance | 32 mm | 16 mm |
| Run time | 23 min | 14 min |
| Space between well centers | 4.5 mm | 9 mm |
| Sample volume | 15 µL | 20 µl |
| Maximum protein load | 20 µg | 20 µg |
Migration pattern of Invitrogen E-PAGE MagicMark Unstained Protein Standard.
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