Protein Gels

All Invitrogen precast protein gels are high-quality polyacrylamide gels designed to deliver excellent performance, reliability, and reproducibility. Our protein gels are offered in four different chemistries. Whether your protein is 2.5 kDa or as large as 500 kDa, or you’re looking for separation under native conditions or by isoelectric point—we have a solution for your application.

Choose Bis-Tris gel chemistry when you have a low abundance protein or when your downstream applications require high protein integrity, such as post-translational modification analysis, mass spectrometry, or sequencing. Bis-Tris chemistry provides a neutral (pH 7.0) environment during electrophoresis, which helps reduce protein modifications and produces sharp band resolution.

Novex Tris-Glycine gel chemistry offers maximum protein separation performance and crisp, straight bands.
For separation of high molecular weight proteins, Tris-acetate gel chemistry is recommended, offered in NuPAGE Tris-Acetate gels.
For separation of low molecular weight proteins, use Novex Tricine gels which provide increased resolution of proteins with molecular weights as low as 2.5 kDa.

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WedgeWell format wells are wedge-shaped wells that increase the sample loading capacity, allowing up to 100 µL of sample to be loaded in the well. The WedgeWell design was introduced in 2012 to increase the sample loading capacity of Invitrogen precast mini gels.

More than a thousand peer-reviewed articles demonstrate how WedgeWell format gels help produce publication-quality results and enable the study of low abundance proteins, reducing the need to enrich dilute samples before loading them onto the gel.

Midi gels with WedgeWell format wells allow the separation of up to 26 protein samples in the same run without compromising on sample loading volume.

Key benefits of WedgeWell format gels:

• Results you can trust—remove run-to-run variability and enhance the reproducibility of your experiments
• Helps retrieve more data in less time—obtain the information you need faster
• Reduced cost—lower cost per sample and reduced running buffer required per sample (7.3 mL of running buffer saved per sample, 140 mL saved for 20 samples)*
• Increased sample loading capacity—easy detection of dilute samples and low abundance proteins
• Easy sample loading—reduced sample spill-over and cross-well contamination

Available in three different gel chemistries:

• Bis- Tris
• Tris-Glycine
• Tris-Acetate

All Invitrogen protein gels meet the highest standards of performance, reliability, and consistency.

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*Based on comparison between our most popular mini and midi well format options (12 and 20 well, respectively)

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For separation of proteins under nondenaturing conditions or native page, three gel systems are recommended- NativePAGE Bis-Tris, Novex Tris-Glycine and NuPAGE Tris-Acetate. NativePAGE Bis-Tris gels, are based on the blue native polyacrylamide gel electrophoresis (BN-PAGE) which helps overcome the limitations of traditional native electrophoresis by providing a near-neutral operating pH and detergent compatibility. Both Novex Tris-Glycine and NuPAGE Tris-Acetate gels can be run under native and non-reducing conditions for separation of proteins by net charge, size and shape.

For separation of proteins by isoelectroctric point (pI), use Novex IEF gels. Novex IEF gels effectively create a pH gradient to separate proteins according to their unique pI.

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1. Libardo, M.D.J.; Durr, E.; Hernandez, L.D. A Robust Protocol to Isolate Outer Membrane Vesicles from Nontypeable Haemophilus influenzae.Methods Protoc.2023,6, 42. https://doi.org/10.3390/mps6020042
2. Horita, H., Law, A., Middleton, K. Utilizing a Comprehensive Immunoprecipitation Enrichment System to Identify an Endogenous Post-translational Modification Profile for Target Proteins. J. Vis. Exp. (131), e56912, doi:10.3791/56912 (2018).