Search Thermo Fisher Scientific
SureCast Gel Handcast System
100% leak-free*: never repour another gel due to leaks
Pour your own polyacrylamide mini gels easily and confidently with the Invitrogen SureCast Gel Handcast System.
System features
- Leak-free* design
- Durable glass plates
- Single component assembly using a single-motion load-and-lock mechanism
- Unique tilt feature- minimizes spillage during gel casting
SureCast Gel Handcast System
| Gel Size | Mini Gel- 8x8 cm (1.0 mm thick) |
| Cassette size | 10cm x 9.8cm x 6.5mm |
| Combs available | 1, 2, 10, 12, 15-well |
| Volume per gel | ~7.7 mL |
| Compatible Equipment | Mini Gel Tank or XCell SureLock Mini-Cell |
| Chemical resistance | Handcast Station is impervious to most alcohols but not compatible with chlorinated hydrocarbons (e.g., chloroform), aromatic hydrocarbons (e.g., toluene, benzene), or acetone. |
SureCast Gel Casting Reagents
| Product specifications | |
|---|---|
| SureCast Stacking Buffer | 0.5 M Tris-HCl buffer, pH 6.8 Dry-blend powder, sufficient to make 500mL stacking gel buffer |
| SureCast Resolving Buffer | 1.5 M Tris buffer, pH 8.8 Dry-blend powder, sufficient to make 500mL resolving buffer |
| SureCast APS | Ammonium persulfate-oxidizing agent that is used with TEMED to catalyze the polymerization of acrylamide and biacrylamide in the preparation of polyacrylamide gels for electrophoresis |
| SureCast Acrylamide Solution (40%) | Concentrated to enable casting of broader range of gel percentages Room temperature storage Safer alternative to powder acrylamide |
| SureCast TEMED | Tetramethylethylenediamine (TEMED), essential catalyst for polyacrylamide gel polymerization |
| SureCast Reagent Product Benefits |
|
Get started with our bundle packs
Welcome packs
SureCast Gel Handcast Bundle A
Savings of up to 15%
Buy now
The Invitrogen SureCast Gel Handcast Bundle A includes all the hardware and reagents to get started:
- 2 SureCast Gel Handcast Stations
- 2 SureCast Glass Plate Sets (2 front, 2 back)
- 2 SureCast Sealing Pads (attached to handcast stations)
- 10 SureCast Gel Spacers
- 1 10-Well Multi-Use Tool
- 1 12-Well Multi-Use Tool
- 1 15-Well Multi-Use Tool
- 6 Gel Combs (2 each: 10-, 12-, 15-well)
- 1 SureCast Stacking Buffer 2-pack
- 1 SureCast Resolving Buffer 2-pack
- 1 SureCast APS (25 g)
- 1 SureCast TEMED (30 mL)
- 1 SureCast Acrylamide Solution, 40%
(450 mL)
SureCast Gel Handcast Bundle B
Savings of up to 18%
Buy now
The Invitrogen SureCast Gel Handcast Bundle B includes all the hardware to get started:
- 2 SureCast Gel Handcast Stations
- 2 SureCast Glass Plate Sets (2 front, 2 back)
- 2 SureCast Sealing Pads (attached to handcast stations)
- 10 SureCast Gel Spacers
- 1 10-Well Multi-Use Tool
- 1 12-Well Multi-Use Tool
- 1 15-Well Multi-Use Tool
- 6 Gel Combs (2 each: 10-, 12-, 15-well)
Discover how to use the SureCast Handcast System
Learn how to pour your own gels using the SureCast system with this detailed step by step video.
SureCast Gel Handcast System assembly
Step 1: Set-up
Set the handcast station on a level surface.
Step 2: Assemble
Assemble the glass plates with the silicone spacer between them so that all the outer edges are flush.
Step 3: Secure
Place the glass plate assembly in the handcast station. Hold the glass plate assembly to prevent shifting, and close the handle to secure the glass plates in the handcast station.
Step 4: Pour
Tilt the handcast station to recline on the heel. Add resolving gel solution to the glass plates until the solution reaches the level of the fill line.
Allow the resolving gel to polymerize (5–10 min). Verify polymerization.
Tilt the handcast station to recline on the heel. Add the stacking gel solution until it reaches the upper edge of the front glass plate. Set the handcast station back to the upright position.
Step 5: Finish
Insert the comb slowly by starting at one end and sliding it between the glass plates until both ends are in place. Allow the stacking gel to polymerize (5–10 min). Verify polymerization.
Then, release the clamp and remove plate. The gel is ready for use.
Gel casting recipes with SureCast reagents
Choose the polyacrylamide percentage for your application based upon the expected molecular weight of your protein of interest and the volume of your sample. Resolve large molecules with low percentage gels and small molecules with high percentage gels. If the molecular weight of the molecule is unknown, or the sample contains a wide range of molecules, use a mid-range percentage gel.
Prepare resolving gel solution according to the volumes in the following table. The volumes provided in the table are for a single gel. Scale volumes proportionally based on the number of gels to be cast.
Note: Solutions do not require degassing.
| Polyacrylamide % | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Solution | 4% | 6% | 8% | 10% | 12% | 14% | 16% | 18% | 20% |
| SureCast Acrylamide (40%) | 0.8mL | 1.2mL | 1.6mL | 2.0mL | 2.4mL | 2.8mL | 3.3mL | 3.6mL | 4.0mL |
| SureCast Resolving Buffer | 2.0mL | 2.0mL | 2.0mL | 2.0mL | 2.0mL | 2.0mL | 2.0mL | 2.0mL | 2.0mL |
| Distilled water | 5.1mL | 4.7mL | 4.3mL | 3.9mL | 3.5mL | 3.1mL | 2.7mL | 2.3mL | 1.9mL |
| 10% SureCast APS | 80µL | 80µL | 80µL | 80µL | 80µL | 80µL | 80µL | 80µL | 80µL |
| SureCast TEMED** | 8µL | 8µL | 8µL | 8µL | 8µL | 8µL | 8µL | 8µL | 8µL |
**Add this last and mix well just before the gel is to be poured
Prepare stacking gel solution according to the following table. The volumes provided in the table are for a single gel. Scale volumes proportionally based on the number of gels to be cast. Note: Solutions do not require degassing.
| Solution | 4% |
|---|---|
| SureCast Acrylamide (40%) | 0.30 mL |
| SureCast Stacking Buffer | 0.75 mL |
| Distilled water | 1.92 mL |
| 10% SureCast APS | 30 µL |
| SureCast TEMED*** | 3 µL |
***Add this last and mix well just before the gel is to be poured
SureCast “100% Leak Free” claim is based on internal testing, including, but not limited to 240 gel pours and over 200 stress tests. The SureCast Handcast System and its components are designed and manufactured to be leak free. Leak free is defined as having less than 1.0 mm drop in liquid polyacrylamide gel casting solution before polymerization when all hardware parts and components are in good material condition (all components free from damage or excessive wear) and used according to manufacturer’s specifications.
Only the following components are covered by the SureCast “100% Leak Free” Statement: SureCast Handcast Station, SureCast Glass Plates, SureCast Gel Spacers and SureCast Sealing Pads. SureCast reagents are expressly excluded from the SureCast “100% Leak Free” Statement.
If a SureCast Handcast System component does not meet the above qualifications, Thermo Fisher Scientific, at its option, will either replace it at no cost or credit your account with the purchase price of the component or system. Thermo Fisher Scientific reserves the right to replace or provide credit for each component individually and is not obliged to replace or provide credit for the entire system. Customer may use such credit to purchase other protein gel electrophoresis products from Thermo Fisher Scientific. REPLACEMENT OR CREDIT OF THE PURCHASE PRICE AS SET FORTH ABOVE IS THERMO FISHER SCIENTIFIC’S SOLE OBLIGATION AND CUSTOMER'S SOLE AND EXCLUSIVE REMEDY WITH RESPECT TO FAILURE OF THE SYSTEM TO MEET THE “100% LEAK FREE” STATEMENT. Customer must contact Thermo Fisher Scientific Technical Support to submit any claims. Thermo Fisher Scientific’s experienced support team will help customer to identify the issue and replace the component or credit customer’s account as needed.
Any and all claims should be made within 12 months of delivery. Any claims not made by the customer within the applicable period will be forfeited. SureCast component coverage is limited to the original buyer of the product from Thermo Fisher Scientific and is not transferable.
Except for the foregoing SureCast “100% Leak Free” Statement, Thermo Fisher Scientific does not make any other guarantee, warranty, or representation with respect to the above system notwithstanding anything to the contrary in any product documentation. The SureCast “100% Leak Free” Statement is Thermo Fisher Scientific’s sole and exclusive guarantee with respect to such system and is in lieu of all other guarantees, warranties, or representations. Thermo Fisher Scientific DISCLAIMES ALL OTHER WARRANTIES, GUARANTEES, RESPRESENTIONS, EXPRESS OR IMPLIED, INCLUDING THE IMPLIED WARRANTY OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, OR NON-INFRINGEMENT. AND WE MAKE NO OTHER REPRESENTATIONS OR WARRANTIES OF ANY KIND WHATSOEVER, EXPRESS OR IMPLIED, INCLUDING WITHOUT LIMITATION ANY IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OF NON-INFRINGEMENT, OR REGARDING RESULTS OBTAINED THROUGH THE USE OF ANY PRODUCT OR SERVICE, WHETHER ARISING FROM A STATUTE OR OTHERWISE IN LAW OR FROM A COURSE OF PERFORMANCE, DEALING OR USAGE OF TRADE, ALL OF WHICH ARE EXPRESSLY DISCLAIMED.
The SureCast “100% Leak Free” Statement does not cover, and Thermo Fisher Scientific has no obligation replace or provide credit if a component does not meet the SureCast “100% Leak Free” Statement because of the customer’s failure to store, maintain or use the product in accordance with recommended protocol for the related system and components; failure to use the product in accordance with good laboratory practices by technically qualified persons; other misuse or neglect, including but not limited to improper storage, maintenance, shipping, or handling, improper or abnormal use, including operation outside of the environmental or use specifications specified by Thermo Fisher Scientific; accidents of any kind or nature; adulteration; unauthorized change or modification; and use with chemicals, reagents or any other product not supplied or authorized in writing by Thermo Fisher Scientific for use with the product, provided that Thermo Fisher Scientific may at its sole discretion honor its SureCast “100% Leak Free” Statement used by customer with non-Thermo Fisher Scientific products.
To the fullest extent allowed by law, in no event shall Thermo Fisher Scientific be liable, whether in contract, tort, strict liability, negligence, warranty, or under any statute or any other basis for any special, incidental, indirect, exemplary, punitive, multiple or consequential damages sustained by customer or any other person or entity arising out of or caused by the system or other products, Thermo Fisher Scientific performance or failure to perform its obligations relating to the purchase, possession, or use of the system or performance of services, whether foreseeable and whether or not Thermo Fisher Scientific is advised of the possibility of such damages, including without limitation damages arising from or related to loss or use, loss of data, downtime, procurement of substitute goods or services, or for loss of revenue, profits, goodwill, or business or other financial loss. In addition, Thermo Fisher Scientific maximum aggregate liability arising out of or in connection with the failure of Thermo Fisher Scientific to the SureCast “100% Leak Free” Statement is limited to the amount customer paid for the system or component that gives rise to such claim. Some countries or jurisdictions limit the scope of or preclude limitations or exclusion of warranties, of liability, such as liability for gross negligence or willful misconduct, or of remedies or damages, as or to the extent set forth above. In such countries and jurisdictions, the limitation or exclusion of warranties, liability, remedies or damages set forth above shall apply to the fullest extent permitted by law, and shall not apply to the extent prohibited by law. Without limiting the foregoing statement, nothing in this document is intended to or will exclude or limit Thermo Fisher Scientific's liability for death or personal injury caused by its negligence or fraud, or any other liability to the extent that the same may not be excluded or limited by applicable law.
Thermo Fisher Scientific reserves the right to add or remove products covered by the SureCast “100% Leak Free” Statement, to modify the terms or terminate the SureCast “100% Leak Free” Statement without prior notice to customers.
SureCast Gel Handcast System
| Gel Size | Mini Gel- 8x8 cm (1.0 mm thick) |
| Cassette size | 10cm x 9.8cm x 6.5mm |
| Combs available | 1, 2, 10, 12, 15-well |
| Volume per gel | ~7.7 mL |
| Compatible Equipment | Mini Gel Tank or XCell SureLock Mini-Cell |
| Chemical resistance | Handcast Station is impervious to most alcohols but not compatible with chlorinated hydrocarbons (e.g., chloroform), aromatic hydrocarbons (e.g., toluene, benzene), or acetone. |
SureCast Gel Casting Reagents
| Product specifications | |
|---|---|
| SureCast Stacking Buffer | 0.5 M Tris-HCl buffer, pH 6.8 Dry-blend powder, sufficient to make 500mL stacking gel buffer |
| SureCast Resolving Buffer | 1.5 M Tris buffer, pH 8.8 Dry-blend powder, sufficient to make 500mL resolving buffer |
| SureCast APS | Ammonium persulfate-oxidizing agent that is used with TEMED to catalyze the polymerization of acrylamide and biacrylamide in the preparation of polyacrylamide gels for electrophoresis |
| SureCast Acrylamide Solution (40%) | Concentrated to enable casting of broader range of gel percentages Room temperature storage Safer alternative to powder acrylamide |
| SureCast TEMED | Tetramethylethylenediamine (TEMED), essential catalyst for polyacrylamide gel polymerization |
| SureCast Reagent Product Benefits |
|
Get started with our bundle packs
Welcome packs
SureCast Gel Handcast Bundle A
Savings of up to 15%
Buy now
The Invitrogen SureCast Gel Handcast Bundle A includes all the hardware and reagents to get started:
- 2 SureCast Gel Handcast Stations
- 2 SureCast Glass Plate Sets (2 front, 2 back)
- 2 SureCast Sealing Pads (attached to handcast stations)
- 10 SureCast Gel Spacers
- 1 10-Well Multi-Use Tool
- 1 12-Well Multi-Use Tool
- 1 15-Well Multi-Use Tool
- 6 Gel Combs (2 each: 10-, 12-, 15-well)
- 1 SureCast Stacking Buffer 2-pack
- 1 SureCast Resolving Buffer 2-pack
- 1 SureCast APS (25 g)
- 1 SureCast TEMED (30 mL)
- 1 SureCast Acrylamide Solution, 40%
(450 mL)
SureCast Gel Handcast Bundle B
Savings of up to 18%
Buy now
The Invitrogen SureCast Gel Handcast Bundle B includes all the hardware to get started:
- 2 SureCast Gel Handcast Stations
- 2 SureCast Glass Plate Sets (2 front, 2 back)
- 2 SureCast Sealing Pads (attached to handcast stations)
- 10 SureCast Gel Spacers
- 1 10-Well Multi-Use Tool
- 1 12-Well Multi-Use Tool
- 1 15-Well Multi-Use Tool
- 6 Gel Combs (2 each: 10-, 12-, 15-well)
Discover how to use the SureCast Handcast System
Learn how to pour your own gels using the SureCast system with this detailed step by step video.
SureCast Gel Handcast System assembly
Step 1: Set-up
Set the handcast station on a level surface.
Step 2: Assemble
Assemble the glass plates with the silicone spacer between them so that all the outer edges are flush.
Step 3: Secure
Place the glass plate assembly in the handcast station. Hold the glass plate assembly to prevent shifting, and close the handle to secure the glass plates in the handcast station.
Step 4: Pour
Tilt the handcast station to recline on the heel. Add resolving gel solution to the glass plates until the solution reaches the level of the fill line.
Allow the resolving gel to polymerize (5–10 min). Verify polymerization.
Tilt the handcast station to recline on the heel. Add the stacking gel solution until it reaches the upper edge of the front glass plate. Set the handcast station back to the upright position.
Step 5: Finish
Insert the comb slowly by starting at one end and sliding it between the glass plates until both ends are in place. Allow the stacking gel to polymerize (5–10 min). Verify polymerization.
Then, release the clamp and remove plate. The gel is ready for use.
Gel casting recipes with SureCast reagents
Choose the polyacrylamide percentage for your application based upon the expected molecular weight of your protein of interest and the volume of your sample. Resolve large molecules with low percentage gels and small molecules with high percentage gels. If the molecular weight of the molecule is unknown, or the sample contains a wide range of molecules, use a mid-range percentage gel.
Prepare resolving gel solution according to the volumes in the following table. The volumes provided in the table are for a single gel. Scale volumes proportionally based on the number of gels to be cast.
Note: Solutions do not require degassing.
| Polyacrylamide % | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Solution | 4% | 6% | 8% | 10% | 12% | 14% | 16% | 18% | 20% |
| SureCast Acrylamide (40%) | 0.8mL | 1.2mL | 1.6mL | 2.0mL | 2.4mL | 2.8mL | 3.3mL | 3.6mL | 4.0mL |
| SureCast Resolving Buffer | 2.0mL | 2.0mL | 2.0mL | 2.0mL | 2.0mL | 2.0mL | 2.0mL | 2.0mL | 2.0mL |
| Distilled water | 5.1mL | 4.7mL | 4.3mL | 3.9mL | 3.5mL | 3.1mL | 2.7mL | 2.3mL | 1.9mL |
| 10% SureCast APS | 80µL | 80µL | 80µL | 80µL | 80µL | 80µL | 80µL | 80µL | 80µL |
| SureCast TEMED** | 8µL | 8µL | 8µL | 8µL | 8µL | 8µL | 8µL | 8µL | 8µL |
**Add this last and mix well just before the gel is to be poured
Prepare stacking gel solution according to the following table. The volumes provided in the table are for a single gel. Scale volumes proportionally based on the number of gels to be cast. Note: Solutions do not require degassing.
| Solution | 4% |
|---|---|
| SureCast Acrylamide (40%) | 0.30 mL |
| SureCast Stacking Buffer | 0.75 mL |
| Distilled water | 1.92 mL |
| 10% SureCast APS | 30 µL |
| SureCast TEMED*** | 3 µL |
***Add this last and mix well just before the gel is to be poured
SureCast “100% Leak Free” claim is based on internal testing, including, but not limited to 240 gel pours and over 200 stress tests. The SureCast Handcast System and its components are designed and manufactured to be leak free. Leak free is defined as having less than 1.0 mm drop in liquid polyacrylamide gel casting solution before polymerization when all hardware parts and components are in good material condition (all components free from damage or excessive wear) and used according to manufacturer’s specifications.
Only the following components are covered by the SureCast “100% Leak Free” Statement: SureCast Handcast Station, SureCast Glass Plates, SureCast Gel Spacers and SureCast Sealing Pads. SureCast reagents are expressly excluded from the SureCast “100% Leak Free” Statement.
If a SureCast Handcast System component does not meet the above qualifications, Thermo Fisher Scientific, at its option, will either replace it at no cost or credit your account with the purchase price of the component or system. Thermo Fisher Scientific reserves the right to replace or provide credit for each component individually and is not obliged to replace or provide credit for the entire system. Customer may use such credit to purchase other protein gel electrophoresis products from Thermo Fisher Scientific. REPLACEMENT OR CREDIT OF THE PURCHASE PRICE AS SET FORTH ABOVE IS THERMO FISHER SCIENTIFIC’S SOLE OBLIGATION AND CUSTOMER'S SOLE AND EXCLUSIVE REMEDY WITH RESPECT TO FAILURE OF THE SYSTEM TO MEET THE “100% LEAK FREE” STATEMENT. Customer must contact Thermo Fisher Scientific Technical Support to submit any claims. Thermo Fisher Scientific’s experienced support team will help customer to identify the issue and replace the component or credit customer’s account as needed.
Any and all claims should be made within 12 months of delivery. Any claims not made by the customer within the applicable period will be forfeited. SureCast component coverage is limited to the original buyer of the product from Thermo Fisher Scientific and is not transferable.
Except for the foregoing SureCast “100% Leak Free” Statement, Thermo Fisher Scientific does not make any other guarantee, warranty, or representation with respect to the above system notwithstanding anything to the contrary in any product documentation. The SureCast “100% Leak Free” Statement is Thermo Fisher Scientific’s sole and exclusive guarantee with respect to such system and is in lieu of all other guarantees, warranties, or representations. Thermo Fisher Scientific DISCLAIMES ALL OTHER WARRANTIES, GUARANTEES, RESPRESENTIONS, EXPRESS OR IMPLIED, INCLUDING THE IMPLIED WARRANTY OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, OR NON-INFRINGEMENT. AND WE MAKE NO OTHER REPRESENTATIONS OR WARRANTIES OF ANY KIND WHATSOEVER, EXPRESS OR IMPLIED, INCLUDING WITHOUT LIMITATION ANY IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OF NON-INFRINGEMENT, OR REGARDING RESULTS OBTAINED THROUGH THE USE OF ANY PRODUCT OR SERVICE, WHETHER ARISING FROM A STATUTE OR OTHERWISE IN LAW OR FROM A COURSE OF PERFORMANCE, DEALING OR USAGE OF TRADE, ALL OF WHICH ARE EXPRESSLY DISCLAIMED.
The SureCast “100% Leak Free” Statement does not cover, and Thermo Fisher Scientific has no obligation replace or provide credit if a component does not meet the SureCast “100% Leak Free” Statement because of the customer’s failure to store, maintain or use the product in accordance with recommended protocol for the related system and components; failure to use the product in accordance with good laboratory practices by technically qualified persons; other misuse or neglect, including but not limited to improper storage, maintenance, shipping, or handling, improper or abnormal use, including operation outside of the environmental or use specifications specified by Thermo Fisher Scientific; accidents of any kind or nature; adulteration; unauthorized change or modification; and use with chemicals, reagents or any other product not supplied or authorized in writing by Thermo Fisher Scientific for use with the product, provided that Thermo Fisher Scientific may at its sole discretion honor its SureCast “100% Leak Free” Statement used by customer with non-Thermo Fisher Scientific products.
To the fullest extent allowed by law, in no event shall Thermo Fisher Scientific be liable, whether in contract, tort, strict liability, negligence, warranty, or under any statute or any other basis for any special, incidental, indirect, exemplary, punitive, multiple or consequential damages sustained by customer or any other person or entity arising out of or caused by the system or other products, Thermo Fisher Scientific performance or failure to perform its obligations relating to the purchase, possession, or use of the system or performance of services, whether foreseeable and whether or not Thermo Fisher Scientific is advised of the possibility of such damages, including without limitation damages arising from or related to loss or use, loss of data, downtime, procurement of substitute goods or services, or for loss of revenue, profits, goodwill, or business or other financial loss. In addition, Thermo Fisher Scientific maximum aggregate liability arising out of or in connection with the failure of Thermo Fisher Scientific to the SureCast “100% Leak Free” Statement is limited to the amount customer paid for the system or component that gives rise to such claim. Some countries or jurisdictions limit the scope of or preclude limitations or exclusion of warranties, of liability, such as liability for gross negligence or willful misconduct, or of remedies or damages, as or to the extent set forth above. In such countries and jurisdictions, the limitation or exclusion of warranties, liability, remedies or damages set forth above shall apply to the fullest extent permitted by law, and shall not apply to the extent prohibited by law. Without limiting the foregoing statement, nothing in this document is intended to or will exclude or limit Thermo Fisher Scientific's liability for death or personal injury caused by its negligence or fraud, or any other liability to the extent that the same may not be excluded or limited by applicable law.
Thermo Fisher Scientific reserves the right to add or remove products covered by the SureCast “100% Leak Free” Statement, to modify the terms or terminate the SureCast “100% Leak Free” Statement without prior notice to customers.
완벽한 100% 누출 방지 설계
Never have to repour another gel due to leaks
Invitrogen™ SureCast™ Gel Handcast System을 이용해 사용자가 직접 쉽고 자신 있게 polyacrylamide mini-gel을 부을 수 있습니다. 이 시스템에는 한 번 로딩하면 잠기는(load-and-lock) 장치를 사용해 안전하게 밀폐할 수 있는 매우 튼튼한 유리판이 들어 있습니다. SureCast Gel Handcast Station만의 고유한 기울임 기능은 사용자가 직접 캐스팅(casting)하는 동안 흘림을 최대한 방지해 줍니다. SureCast Station을 통해 조제된 겔은 당사 Invitrogen™ Mini Gel Tank 에서 사용할 수 있습니다.
SureCast Gel Handcast System의 특징:
- 누출 방지 설계—겔 제조 실패 횟수를 최소화함으로써 폐기물을 줄이고 시간을 절약함
- 우수한 유리판 내구성—수년 간 안정적인 성능을 발휘함
- 고유한 기울임 기능—흘림을 최대한 방지하므로 acrylamide 용액을 자신 있게 로딩할 수 있음
- 간편한 구성품 조립—한 번 로딩하면 잠기는(load-and-lock) 장치 사용
- 호환성—SureCast 시약이나 다른 polyacrylamide gel-casting 시약과 함께 사용 가능
**Based on internal testing
Customer story
"We have used the SureCast system for one semester and I love them! The system is easy to use; the students can have the system set up in no time without any assistance from the TAs or myself and it does not leak. With the (other supplier’s) system, the TAs and I would be running around the lab trying to stop leaking gels, or showing students how to set up the apparatus properly, even after giving a demo to the class. This past semester, the TAs and I sat down and watched the students work! The gels themselves are bigger and thicker - just because I used to use 0.75mm spacers and the ones provided with the SureCast system are 1mm spacers- so I do go through more acrylamide, but not significantly more because of all the leaking with the old system.
As far as the tanks go, I love them as well. Advantages are that the gels are side by side, so it is easier for students to load and therefore speeds up the lab. Additionally, no blank plate is required for a dam if there are an odd number of gels being used. That simplifies things for the students as well.
Honestly, I am so happy we converted and regret it wasn't done sooner."
—Amanda van der Vinne, Undergraduate Teaching Coordinator, University of Guelph
Resources
평평한 표면에 Handcast Station을 놓습니다.
유리판 사이에 실리콘 스페이서를 넣고 모든 외부 가장자리가 잘 맞도록 유리판을 조립합니다.
Handcast Station에 유리판 조립품을 배치합니다. 이동하지 않도록 유리판 조립품을 고정하고, 손잡이를 닫아 Handcast Station에 유리판을 고정합니다.
 |  |  | ||
| Handcast Station의 비스듬한 뒤쪽 면을 바닥에 두어 Handcast Station을 기울입니다. 채움선(fill line)에 도달할 때까지 resolving gel 용액을 유리판에 붓습니다. | Resolving gel이 중화되도록 둡니다(5-10분). 중화를 확인합니다. | Handcast Station의 비스듬한 뒤쪽 면을 바닥에 두어 Handcast Station을 기울입니다. Stacking gel 용액이 유리판 앞면 가장자리에 올 때까지 붓습니다. Handcast Station을 다시 똑바로 세웁니다. |
한쪽 끝부터 콤(comb)을 천천히 삽입하기 시작하여 양쪽 끝에 적절히 놓일 때까지 유리판 사이에 밀어 넣습니다. Stacking gel이 중화되도록 둡니다(5-10분). 중화를 확인합니다.
그리고 나서 클램프를 풀고 유리판을 꺼냅니다. 이제, 갤을 바로 사용할 수 있습니다.
평평한 표면에 Handcast Station을 놓습니다.
유리판 사이에 실리콘 스페이서를 넣고 모든 외부 가장자리가 잘 맞도록 유리판을 조립합니다.
Handcast Station에 유리판 조립품을 배치합니다. 이동하지 않도록 유리판 조립품을 고정하고, 손잡이를 닫아 Handcast Station에 유리판을 고정합니다.
| | | ||
| Handcast Station의 비스듬한 뒤쪽 면을 바닥에 두어 Handcast Station을 기울입니다. 채움선(fill line)에 도달할 때까지 resolving gel 용액을 유리판에 붓습니다. | Resolving gel이 중화되도록 둡니다(5-10분). 중화를 확인합니다. | Handcast Station의 비스듬한 뒤쪽 면을 바닥에 두어 Handcast Station을 기울입니다. Stacking gel 용액이 유리판 앞면 가장자리에 올 때까지 붓습니다. Handcast Station을 다시 똑바로 세웁니다. |
한쪽 끝부터 콤(comb)을 천천히 삽입하기 시작하여 양쪽 끝에 적절히 놓일 때까지 유리판 사이에 밀어 넣습니다. Stacking gel이 중화되도록 둡니다(5-10분). 중화를 확인합니다.
그리고 나서 클램프를 풀고 유리판을 꺼냅니다. 이제, 갤을 바로 사용할 수 있습니다.
SureCast gel-handcasting 시약을 사용하는 이유는?
Invitrogen™ SureCast™ Stacking Buffer 와 Invitrogen™ SureCast™ Resolving Buffer 는 파우치 형태의 건식 혼합 분말로서, 각각 500mL의 stacking gel buffer(0.5M Tris-HCl buffer, pH 6.8) 또는 500mL의 resolving gel buffer(1.5M Tris buffer, pH 8.8)를 제조하여 polyacrylamide 겔을 직접 캐스팅(casting)하는 데 적합합니다.
장점:
- 편리한 파우치 형태의 건식 혼합 분말—한 통의 내용물을 물에 용해하여 버퍼를 즉시 사용할 수 있음
- 시간과 공간 절약—칭량, 계산, pH 조절이 필요 없고, 각각의 구성시약을 비축하지 않아도 됨
- 긴 유효기간—건식 분말 형태로 비축 및 보관하므로 원액의 장기 안정성과 관련된 문제를 걱정하지 않아도 됨
SureCast polyacrylamide solution 을 사용하면 SureCast Gel Handcast Station이나 다른 핸드캐스팅(handcasting) 시스템을 사용해 단일 비율(%)과 gradient gel을 조제할 수 있습니다.
특징:
- 실온 보관
- 긴 유효기간
- 고순도
- 분말 acrylamide의 안전한 대안
- 농축(40%) 형태로 보다 넓은 범위의 겔 비율로 캐스팅할 수 있음
SureCast Gel Handcast Station 사양
| 용량 | mini-gel 1개 조제를 위한 유리판 2개와 silicone spacer 1개 |
| 셀 크기 | 13 x 10 x 12.5cm(손잡이를 앞으로 당겼을 때의 높이) |
| 겔당 용적 | 약 7.7mL |
| 소재 | Polycarbonate, acetal, polypropylene, silicone |
| 내화학성 | SureCast Gel Handcast Station은 대다수 알코올에 영향을 받지 않지만, 염소화 탄화수소(클로로폼 등), 방향족 탄화수소(톨루엔, 벤젠 등), 아세톤은 사용할 수 없습니다. |
| 조립 후 카세트 크기 | 10cm x 9.8cm x 6.5mm (당사 Mini Gel Tank와 함께 사용할 수 있도록 제작됨) |
| 제조 후 겔 크기 | 8cm x 8cm x 1mm(mini-gel) |
For Research Use Only. Not for use in diagnostic procedures.