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Invitrogen Novex Tris-Glycine Gels are polyacrylamide gels based on traditional Laemmli protein electrophoresis. Novex Tris-Glycine Gels offer reproducible separation of a wide range of proteins into well-resolved bands.
Key features of the Novex Tris-Glycine Gels include:
Available gel sizes | Mini: 8 cm x 8 cm (1.0 mm thick) Midi: 8 cm x 13 cm (1.0 mm thick) |
Available Well configurations* | WedgeWell format, Midi (load up to 100 µL per well): 12+2, 20, 26 wells WedgeWell format, Mini (load up to 60 µL per well): 10, 12, 15 wells Midi: 12+2, 20, 26 wells Mini: 10, 12, 15 wells |
Storage conditions | 2–8°C |
Shelf life | Up to 12 months |
Recommended sample buffer | SDS-PAGE: Novex Tris-Glycine SDS Sample Buffer Native-PAGE: Novex Tris-Glycine Native Sample Buffer |
Recommended running buffers | SDS-PAGE: Novex Tris-Glycine SDS Running Buffer Native-PAGE: Novex Tris-Glycine Native Running Buffer |
Recommended transfer buffers | Novex Tris-Glycine Transfer Buffer |
Gel chemistry | Tris-glycine |
Available polyacrylamide concentrations | 6%, 8%, 10%, 12%, 14%, 16%, 4–12%, 4–20%, 8–16%, 10–20% |
Separation range (denaturing) | 8–250 kDa |
For use with (equipment) mini gels | Mini Gel Tank or XCell SureLock Mini-Cell |
For use with (equipment) midi gels | SureLock Tandem Midi Gel Tank, Invitrogen XCell4 SureLock Midi-Cell or Bio-Rad Criterion (with adapters only) |
Mode of separation | SDS-PAGE: Molecular weight Native-PAGE: Intrinsic charge, molecular size |
Applications | SDS-PAGE, Native-PAGE |
*Not all percentages are available in every well type
Novex Tris-Glycine Gels do not contain SDS and can be used to run your proteins in native or in denatured form. For denatured proteins, we recommend using tris-glycine SDS sample buffer and a tris-glycine SDS running buffer. For native proteins, we recommend using a tris-glycine native sample buffer and a tris-glycine native running buffer.
Novex Tris-Glycine Gels use a tris-glycine discontinuous buffer system with three ions primarily involved:
WedgeWell format wells are wedge-shaped wells that increase the sample loading capacity, allowing up to 60 µL and 100 µL of samples to be loaded in the mini and midi gel wells, respectively. WedgeWell Midi format gels that combine the higher sample throughput of wide format gels with the higher sample loading capacity of WedgeWells are now available. Key benefits include:
Well size | Recommended loading volume (µL) | Maximum loading volume (µL) |
---|---|---|
WedgeWell format midi gels | ||
12 + 2 (large well) | 90 | 100 |
12 + 2 (small well) | 30 | 35 |
20 | 50 | 60 |
26 | 30 | 40 |
WedgeWell format mini gels | ||
10 | 40 | 60 |
12 | 30 | 45 |
15 | 20 | 35 |
17 | 15 | 30 |
Midi gels | ||
12 + 2 (large well) | 45 | 60 |
12 + 2 (small well) | 15 | 20 |
20 | 25 | 30 |
26 | 15 | 20 |
Mini gels* | ||
9 | 17 | 28 |
10 | 15 | 25 |
12 | 12 | 20 |
15 | 9 | 15 |
17 | 9 | 15 |
WedgeWell format well increases the well sample loading capacity, helping prevent sample spillover and cross-contamination.
Figure 1. Increased sample volume capacity of Novex Tris-glycine midi WedgeWell format gels. (A) To compare gel spillover, increasing volumes (20–60 μL) of a fluorescent protein ladder were loaded in every other lane of a Novex WedgeWell Tris-glycine midi 20-well gel (left) or Bio-Rad 4–20% Criterio TGX Protein Gel (right). Sample spillover in Bio-Rad’s gel is seen in lanes adjacent to the 40–60 μL loading lanes.
The amount of protein you can load into a protein gel well affects the ability to detect the protein following protein gel electrophoresis or western blotting; the more you can load, the easier it is to detect. The protein load capacities of Invitrogen Novex Tris-Glycine Bis-Tris precast midi gels run in the SureLock Tandem Midi Gel Tank were compared against Bio-Rad Criterion midi gels run in a Bio-Rad Criterion Cell Midi Cell Tank using manufacturer instructions. Decreasing amounts of HEK 293 cell lysate prepared in RIPA lysis buffer (48–0.5 µg total protein) were denatured in the respective manufacturer’s sample buffer and subjected to electrophoresis using manufacturer instructions. The table below lists the samples, protein mass, and %RIPA buffer loaded in each lane.
Invitrogen Novex Tris-Glycine gels outperformed Bio-Rad gels at higher lysate loads with blots from Bio-Rad gels showing band loss and smearing at higher loads for all targets investigated.
Lane | +1 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | +1 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Sample | Sample buffer | iBright Protein Ladder | HEK293 lysate | iBright Protein Ladder | Sample buffer | |||||||||
Load mass (µg) | NA | NA | 60 | 50 | 40 | 30 | 20 | 10 | 5 | 2 | 1 | 0.5 | NA | NA |
Load vol. (µL) | 5 | 3+25 S.B. | 30 | 30 | 30 | 30 | 30 | 30 | 30 | 30 | 30 | 30 | 3+25 S.B. | 5 |
Conc. (µg/µL) | NA | NA | 2 | 1.67 | 1.33 | 1 | 0.67 | 0.33 | 0.17 | 0.07 | 0.03 | 0.02 | NA | NA |
Novex Tris-Glycine Plus, Midi, WedgeWell format gels offer increased loading capacity and excellent protein separation.
Figure 2. The protein and RIPA buffer load capacity of Novex 4–20% Tris-Glycine Plus, Midi, 12+2 well WedgeWell format gel exceeds that of Bio-Rad gels. After loading and electrophoresis, a Novex 4–20% Tris-Glycine Plus midi, 12+2 well, WedgeWell format gel, and a Bio-Rad 4–20% Criterion TGX , 12+2 well, midi gel were stained with SimplyBlue SafeStain. The Bio-Rad gel succumbs to protein and lysis buffer overload above a load of 20 µg protein, resulting in streaking and smearing. Staining near the wells indicates some protein has had difficulty entering the gel. The Novex 4–20% Tris-Glycine Plus midi gel provides better protein band sharpness and resolution versus the Bio-Rad 4–20% TGX gel under these loading conditions.
Figure 3. Western blots using Novex 4–20% Tris-Glycine Plus midi gels display sharper bands at greater protein and RIPA lysis buffer loads than Bio-Rad 4–20% TGX midi gels. A Novex 4–20% Tris-Glycine Plus midi gel, 12+2 well, was loaded with decreasing total protein amount of HEK293 lysate, subjected to electrophoresis in a SureLock Tandem Midi Gel Tank and transferred onto a 0.45 µm PVDF membrane using the SureLock Tandem Blot Module. In parallel, a Bio-Rad 4-20% TGX midi gel, 12+2 well, was subjected to electrophoresis in a Criterion Midi Cell Tank and transferred onto a 0.45 µm PVDF membrane using the Criterion Blotter. Both membranes were analyzed for total protein using the No-Stain Protein Labeling Reagent, followed by chemiluminescent immunodetection of three targets: Vinculin, α-Tubulin, and p23. The Bio-Rad blot shows streaking, bowing of bands above 24 µg protein, and bleed-over into the ladder lane for total protein analysis. With immunoblotting, the Bio-Rad blot shows band loss and smearing at higher loads for all targets. For immunodetection: membranes were blocked for 1 hour in 1X Blocker FL Fluorescent Blocking Buffer. For chemiluminescent detection: the membranes were probed overnight with a mixture of primary antibodies diluted in blocking solution: Rabbit-anti Vinculin (1:30,000), Rat anti-α Tubulin (1:15,000), and Mouse-anti p23 (1:60,000) followed by an incubation with secondary antibodies in 1X Blocker FL: Donkey anti-Rabbit HRP (1:5,000), Donkey anti-Rat HRP (1:30,000), and Donkey anti-Mouse HRP (1:240,000) for 1 hour. Membranes were incubated for 5 minutes with SuperSignal West Dura Extended Duration Substrate and imaged for the same amount of time on an iBright Imaging System.
Novex Tris-Glycine Gels are designed to deliver well-resolved, straight bands with optimal band quality as compared to other commercially available precast gels.
Novex Tris-Glycine Gels are designed to deliver well-resolved, straight bands with optimal band quality as compared to other commercially available precast gels.
Figure 4. Band quality with Novex Tris-Glycine gels. Protein ladders, purified proteins, and E. coli lysate were loaded on an Invitrogen Novex 4–20% Tris-Glycine Mini Gel, (A) and a 4–20% gradient gel (B). Straighter lanes with better lysate protein band sharpness and resolution are observed on gel (A). Lanes 1, 5, 10: 5 µL Thermo Scientific PageRuler Unstained Protein Ladder; lanes 2, 6, 9: 5 µL Invitrogen Mark12 Unstained Standard; lane 3: 10 µg E. coli lysate; lane 4: 6 µg BSA; lane 7: 6 µg hIgG; lane 8: 20 µg E. coli lysate.
Figure 5. Novex Tris-Glycine Gels deliver sharp straight bands. Protein ladders and A431 cell lysate were loaded on a Novex Tris-Glycine Gel, 4–20% gradient and transferred to nitrocellulose using the Invitrogen iBlot 2 Gel Transfer Device. Lane 1: Invitrogen iBright Prestained Protein Ladder; Lane 2: Invitrogen MagicMark XP Western Protein Standard; Lanes 3–7: A431 cell lysate, 15 µg, 5 µg, 1.67 µg, 0.55 µg, 0.19 µg.
With Novex Tris-Glycine Gels, you can achieve greater protein integrity as compared to other commercially available precast gels.
Figure 6. Novex Tris-Glycine Gels offer increased protein integrity. Protein ladder, purified proteins, and E. coli lysate were loaded on a Novex 4–20% Tris-Glycine Mini Gel,(A) and a Bio-Rad TGX 4–20% gradient gel (B). The Bio-Rad TGX gel (B) displays numerous low molecular weight protein degradation products below major bands in lanes 3, 4, 7, 8. These are not seen in the Novex Tris-Glycine gel (A). Gel (A) also displays better lysate protein band sharpness and resolution than gel (B). Lanes 1, 10: 5 µL Mark12 Unstained Standard; lane 2: 10 µg E. coli lysate; lane 3: 6 µg catalase; lane 4: 6 µg carbonic anhydrase; lane 5: 6 µg lysozyme; lane 6: 6 µg hIgM ; lane 7: 6 µg BSA; lane 8: 6 µg beta-galactosidase; lane 9: 20 µg E. coli lysate.
Find an excellent mini or midi gel for your protein electrophoresis experiment.
Find an exceptional Invitrogen mini or midi gel to replace your current precast gel from another supplier.
Download the Protein Gel Guide for a printable gel migration chart.
Invitrogen™ Novex™ WedgeWell™ Tris-Glycine 겔은 polyacrylamide 겔 전기영동(PAGE)을 통해 단백질을 분리하는 데 사용되는 precast mini-gel로서 Laemmli 샘플과 running buffer를 사용할 수 있습니다. 이 겔은 고품질 성능을 보장할 뿐만 아니라 다양한 단백질을 분리능이 우수한 밴드로 분리합니다. Novex WedgeWell Tris-Glycine 겔은 당사의 Invitrogen™ Mini Gel Tank에서 사용할 수 있습니다.
Number of wells | Gel percentage |
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![]() Novex WedgeWell Tris-Glycine 겔 이동 차트각 특정 겔 비율(%)에 최적인 전체 분리 범위는 겔 이동 차트에서 확인하십시오.
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WedgeWell Tris-Glycine 겔의 샘플 용적 수용력 증가. (왼쪽) 증가한 용량 (20 μL–60 μL) 의 형광 단백질 래더를 Invitrogen™ Novex™ WedgeWell Tris-Glycine 10-well 겔의 레인에 하나 걸러씩 로딩했습니다 . (오른쪽) 증가한 용량 (20 μL–60 μL) 의 형광 단백질 래더를 경쟁사 겔의 레인에 하나 걸러씩 로딩했습니다. (B). 겔의 경우, 50μL와 60 μL 로드 레인에 인접한 레인에서 샘플 spillover가 나타났습니다.
Novex WedgeWell Tris-Glycine 겔을 사용한 밴드 품질. 단백질 래더, 정제 단백질, E. coli 용해물을 4–20% gradient WedgeWell Tris-Glycine 겔(A)과 경쟁사 4–20% gradient 겔(B)에 로딩했습니다. 용해물 단백질 밴드 선명도와 분리능이 더 우수하고 더 곧은 레인이 겔 (A)에서 관찰되었습니다. 레인 1, 5, 10: 5µL Thermo Scientific™ PageRuler™ Unstained Protein Ladder, 레인 2, 6, 9: 5µL Mark12 Unstained Standard, 레인 3: 10µg E. coli 용해물(10µL의 샘플 용적), 레인 4: 6µg BSA(10µL의 샘플 용적), 레인 7: 6 µg hlgG(10µL의 샘플 용적), 레인 8: 20µg E. coli 용해물(20µL의 샘플 용적)
Novex WedgeWell Tris-Glycine 겔의 단백질 보전성이 더 우수함. 단백질 래더, 정제 단백질, E. coli 용해물을 4–20% gradient WedgeWell Tris-Glycine 겔(A)과 경쟁사 4–20% gradient 겔(B)에 로딩했습니다. 겔 (B)의 경우, 레인 3, 4, 7, 8의 주요 밴드 아래에서 저분자량 단백질 분리 산물이 다수 나타나 있습니다. 겔 (A)에서는 이러한 결과물이 나타나지 않습니다. 또한, 겔 (A)는 겔 (B)보다 용해물 단백질 밴드 선명도와 분리능이 우수합니다. 레인 1, 10: 5µL Mark12 Unstained Standard, 레인 2: 10µg E. coli 용해물(10µL의 샘플 용적), 레인 3: 6µg catalase(10µL의 샘플 용적), 레인 4: 6µg carbonic anhydrase(10µL의 샘플 용적), 레인 5: 6µg lysozyme(10µL의 샘플 용적), 레인 6: 6µg hIgM(10µL의 샘플 용적), 레인 7: 6µg BSA(10µL의 샘플 용적), 레인 8: 6µg beta-galactosidase(10µL의 샘플 용적), 레인 9: 20µg E. coli 용해물(20µL의 샘플 용적).
WedgeWell Tris-Glycine 겔의 샘플 용적 수용력 증가. (왼쪽) 증가한 용량 (20 μL–60 μL) 의 형광 단백질 래더를 Invitrogen™ Novex™ WedgeWell Tris-Glycine 10-well 겔의 레인에 하나 걸러씩 로딩했습니다 . (오른쪽) 증가한 용량 (20 μL–60 μL) 의 형광 단백질 래더를 경쟁사 겔의 레인에 하나 걸러씩 로딩했습니다. (B). 겔의 경우, 50μL와 60 μL 로드 레인에 인접한 레인에서 샘플 spillover가 나타났습니다.
Novex WedgeWell Tris-Glycine 겔을 사용한 밴드 품질. 단백질 래더, 정제 단백질, E. coli 용해물을 4–20% gradient WedgeWell Tris-Glycine 겔(A)과 경쟁사 4–20% gradient 겔(B)에 로딩했습니다. 용해물 단백질 밴드 선명도와 분리능이 더 우수하고 더 곧은 레인이 겔 (A)에서 관찰되었습니다. 레인 1, 5, 10: 5µL Thermo Scientific™ PageRuler™ Unstained Protein Ladder, 레인 2, 6, 9: 5µL Mark12 Unstained Standard, 레인 3: 10µg E. coli 용해물(10µL의 샘플 용적), 레인 4: 6µg BSA(10µL의 샘플 용적), 레인 7: 6 µg hlgG(10µL의 샘플 용적), 레인 8: 20µg E. coli 용해물(20µL의 샘플 용적)
Novex WedgeWell Tris-Glycine 겔의 단백질 보전성이 더 우수함. 단백질 래더, 정제 단백질, E. coli 용해물을 4–20% gradient WedgeWell Tris-Glycine 겔(A)과 경쟁사 4–20% gradient 겔(B)에 로딩했습니다. 겔 (B)의 경우, 레인 3, 4, 7, 8의 주요 밴드 아래에서 저분자량 단백질 분리 산물이 다수 나타나 있습니다. 겔 (A)에서는 이러한 결과물이 나타나지 않습니다. 또한, 겔 (A)는 겔 (B)보다 용해물 단백질 밴드 선명도와 분리능이 우수합니다. 레인 1, 10: 5µL Mark12 Unstained Standard, 레인 2: 10µg E. coli 용해물(10µL의 샘플 용적), 레인 3: 6µg catalase(10µL의 샘플 용적), 레인 4: 6µg carbonic anhydrase(10µL의 샘플 용적), 레인 5: 6µg lysozyme(10µL의 샘플 용적), 레인 6: 6µg hIgM(10µL의 샘플 용적), 레인 7: 6µg BSA(10µL의 샘플 용적), 레인 8: 6µg beta-galactosidase(10µL의 샘플 용적), 레인 9: 20µg E. coli 용해물(20µL의 샘플 용적).
웰(Well) 유형 | 단백질 로딩 용적 | 최대 로딩 용적 | 최대 단백질 로딩 |
---|---|---|---|
10 well | 40 µL | 60 µL | 0.5 µg/band |
12 well | 30 µL | 45 µL | 0.4 µg/band |
15 well | 20 µL | 35 µL | 0.25 µg/band |
Novex™ WedgeWell Tris-Glycine 겔은 mini-gel(8cm x 8cm) 형태로 출시됩니다. mini-gel은 1.0mm 겔 단위로 이용 가능하고 Invitrogen만의 독점 WedgeWell® 형태로 되어 있어 모든 1.0mm 두께의 Tris-Glycine mini-gel을 수용할 수 있는 최대 샘플 용적을 제공합니다. Novex™ WedgeWell Tris-Glycine 겔의 형식에는 10-well, 12-well, 15-well이 있습니다. 로딩 용적에 대해서는권장 웰(well) 로딩 용적 및 샘플 로딩을 참조하십시오.
Novex WedgeWell Tris-Glycine 겔은 6%~16%의 다양한 고정 농도뿐 아니라, 4–12%, 4–20%, 8–16%, 10–20% 범위의 gradient로도 출시됩니다. 각 상자에는 겔 10개가 들어 있습니다. 단백질을 분리하는 데 사용해야 하는 겔 유형을 확인하려면, 단백질 이동 패턴을 참조하십시오.
Novex WedgeWell Tris-Glycine 겔은 4°C에서 보관해야 하고 유효기간은 최대 1년입니다.
Novex WedgeWell Tris-Glycine 겔은 SDS를 함유하고 있지 않아 native 또는 denatured 형식의 단백질을 running하는 데 사용할 수 있습니다. denatured 단백질의 경우, Invitrogen™ Novex™ Tris-Glycine SDS Sample Buffer와 Invitrogen™Novex™ Tris-Glycine SDS Running Buffer.사용을 권장합니다. 반면에 native 단백질의 경우, Invitrogen™Novex™ Tris-Glycine Native Sample Buffer 와 Invitrogen™ Novex™ Tris-Glycine Native Running Buffer사용을 권장합니다.
겔을 membrane으로 transfer할 경우에는 Invitrogen™Novex™ Tris-Glycine Transfer Buffer.사용을 권장합니다. transfer를 실시할 때에는 Invitrogen™ Mini Blot Module또는 Invitrogen™ iBlot™ 2 Dry Blotting System을 사용할 수 있습니다.
For Research Use Only. Not for use in diagnostic procedures.