StemPro MSC SFM XenoFree | Thermo Fisher Scientific

Xeno-free media specifically formulated for the growth and expansion of human mesenchymal stem cells (hMSC) and human adipose-derived stem cells (hADSC)

Supports hMSC and hADSC expansion for multiple passages
Maintains spindle-shaped morphology
Maintains multilineage mesoderm differentiation potential
Supports primary isolation of hMSCs
StemPro® MSC SFM XenoFree is cGMP compliant facilitating regulatory review/approval

Supports hMSC expansion

Expansion of hMSCs using StemPro MSC SFM XenoFree on CELLstart substrate-coated flasks is comparable to classical medium (DMEM + 10% FBS) (Figure 1).

Figure 1. Expansion of MSCs in StemPro MSC SFM XenoFree. Human Bone Marrow-derived MSCs (BM-MSCs) cultured in StemPro® MSC SFM XenoFree on CELLstart-coated flasks reveal a similar growth rate compared to DMEM + 10% MSC-Qualified FBS with long-term multi-passage capability

Better quality cells—spindle-shaped morphology

hMSCs grown in StemPro MSC SFM XenoFree medium on CELLstart substrate-coated flasks exhibit a less flattened, spindle-shaped morphology (Figure 2).

Figure 2. Human BM-MSC morphology. BM-MSCs expanded in StemPro MSC SFM XenoFree display a conserved spindle-shaped morphology after 9 passages compared to DMEM + 10% MSC-Qualified FBS. Images were captured using a 10X objective.

Maintains multilineage differentiation potential

hMSCs cultured in StemPro® MSC SFM XenoFree retain their tri-lineage mesoderm differentiation potential (Figure 3).

Multilineage differentiation with StemPro MSC medium

Figure 3. Multilineage differentiation. Human MSCs expanded for 5 passages in StemPro® MSC SFM XenoFree retained multipotency as shown by the ability to differentiate into adipocytes, osteoblasts, and chondrocytes. Staining: adipogenesis = Oil Red O (Day 14); chondrogenesis = Alcian blue (Day 14) osteogenesis = alkaline phosphatase (Day 21). Images were captured using a 10X objective.

Primary Isolation of hMSCs

Purified bone marrow mononuclear cells cultured in StemPro MSC SFM XenoFree supplemented with low level pooled human AB serum (2.5%) supported primary BM-MSC expansion with continual propagation (after Passage 0) under serum- and xeno-free conditions (Figure 4).

Figure 4. Primary Human BM-MSC Expansion. Ficoll-purified BM-MNCs cultured in StemPro® MSC SFM XenoFree (on CELLstart-coated flasks) supplemented with low level pooled human AB serum (2.5%) supported primary BM-MSC expansion with continual propagation (after P0) under serum- and xeno-free conditions.