Concentrating Cells

To concentrate cells from a suspension culture (or resuspended cells from monolayer culture):

1. Transfer the cell suspension to a sterile centrifuge tube of appropriate size and centrifuge for 10 minutes at 800 × g.

   Note:  Certain cell lines are very sensitive to centrifugal force.

2. Carefully remove the supernatant without disturbing the cell pellet.
3. Add the desired volume of fresh medium gently to the side of the tube and slowly pipette up and down 2 to 3 times to resuspend the cell pellet.
4. Transfer the cells to the desired, sterile container.