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Growth and attachment factors, natural and recombinant, are used in conjunction with other essential nutrients and hormones to maximize biological stimulation. The optimal conditions and concentrations of growth factor for particular cell types must be empirically determined. The following table provides a summary of conditions for growth and attachment factor supplementation reported to give maximal stimulation for various cell lines. It can be used to establish initial concentrations prior to optimization.
| Cell Line | Cell Type | Growth or Attachment Factor | Suggested Conc. (ng/ml) | Medium | Serum Suppl. |
|---|---|---|---|---|---|
| MCF 7 | Human mammary carcinoma | EGF(a) | 100 | DMEM | 2% FBS |
| HF | Human foreskin fibroblast | EGF(a) | 2 | DMEM | 10% FBS |
| -- | Primary hepatocyte | EGF(a) | 10 | DMEM | 10% FBS |
| WB | Rat hepatic epithelium | EGF(a) | 10 | Richter's MEM | 10% FBS |
| HeLa | Human cervical carcinoma | EGF(a) | 2.5 x104 | DMEM | 10% FBS |
| A431 | Epidermal carcinoma | EGF(a) | 2.5 x 104 | DMEM | 10% FBS |
| -- | Primary human fibroblast | EGF(a) | 1x103 | MEM Earle's | 10% FBS |
| MKN-7 | Human adenocarcinoma | EGF(a) | 50 | DMEM | 8% FBS |
| BALB/c 3T3 | Mouse fibroblast | EGF(a) | 50 | DMEM/F-12 | -- |
| TM-4 | Mouse testes epithelium | EGF(a) | 3 | DMEM/F-12 | -- |
| NRK-49F | Rat fibroblast | EGF(a) | 50 | DMEM/F-12 | -- |
| GH3 | Rat neuroblastoma | bFGF | 1 | F-12 | -- |
| BHK-21 | Baby hamster kidney | bFGF | 3 | DMEM/F-12 | 5% Calf |
For Research Use Only. Not for use in diagnostic procedures.