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Stem Cell Culture Support
Explore our “Getting Started” and “Troubleshooting” sections for solutions to top inquiries and common problems. Browse through our "Guides and Tools" section to access a comprehensive portfolio of product-related support resources.
Getting Started
- How does Gibco Essential 8 Flex Medium work without daily feeding?
- If I intend to transition from the original Gibco Essential 8 Medium to Essential 8 Flex Medium, will I need to prepare the complete medium any differently?
- In the Invitrogen CytoTune-iPS 2.0 Sendai Reprogramming Kit, hKlf4 is on the polycistronic vector. Why do I need extra hKlf4?
- How is Sendai virus different from lentivirus?
- Do I need to use all three reprogramming vectors with the Invitrogen CytoTune-iPS 2.0 Sendai Reprogramming Kit?
Troubleshooting
- I used the Gibco PSC Cardiomyocyte Differentiation Kit and am not seeing good differentiation with my iPSC line. What should I do?
- I am seeing cell death during reprogramming using the Invitrogen CytoTune-iPS 2.0 Sendai Reprogramming Kit? Is this normal?
- What do I do if my iPSCs still contain the Sendai virus after 10 passages?
- Why do you not recommend using valproic acid (VPA) during CytoTune 2.0 or 1.0 reprogramming experiments?
- My neural stem cells (NSCs) died after seeding. What went wrong?
Guides and Tools
Brochure: Stem Cell Research
Guide: Neurobiology Protocol Handbook
Search our database for Product Documents: Manuals, Media Formulations, Citations and References
Search our database for Quality and Safety Documents: MSDS, COA
General
- Overview: Stem Cell Research
- Overview: Stem Cell Culture
- Overview: Stem Cell Differentiation
- Overview: Stem Cell Engineering
- Overview: Stem Cell Characterization and Analysis
- Overview: Stem Cell Therapy Research
- Overview: Disease Modeling using Stem Cells
- Learning Center: Stem Cell Research
- Stem Cell Research Resource Library (useful technical, application, and other learning resources including application notes and methods)
- Learning Center: Cell Culture & Transfection
- Cell Culture & Transfection Resource Library (useful technical, application, and other learning resources including application notes and methods)
- Citations: Stem Cell Research Publications
- Poster: Stem Cell Research Posters
- Protocol: Stem Cell Culture Protocols
- Protocol: Stem Cell Engineering Protocols
- Protocol: Stem Cell Characterization
- Reference: Primary and Stem Cells: Gene Transfer Technologies and Applications
- Reference: Emerging Technology Platforms for Stem Cells
- Reference: Regulatory Networks in Stem Cells
- Training: Gibco Virtual Training Labs
- Video: Stem Cell Research Videos
Embryonic Stem Cells (ESCs)
- Overview: Embryonic Stem Cells (ESCs)
Induced Pluripotent Stem Cells (iPSCs)
- Overview: Induced Pluripotent Stem Cells (iPSCs)
- Overview: Culture Systems and Reagents for Neural Differentiation
- Overview: Culture Systems and Reagents for Cardiomyocyte Differentiation
- Newsletter: Live-cell Staining of Pluripotent Stem Cells using Alkaline Phosphatase Live Stain
- Protocol: Pluripotent Stem Cell Protocols
- Selection: Pluripotent Stem Cell Culture Media
- Selection: Extracellular Matrices for Pluripotent Stem Cell Culture
- Selection: Passaging Pluripotent Stem Cells
- Selection: Stem Cell Reprogramming
- Selection: Characterization of Pluripotent Stem Cells
- Video: How to Differentiate Pluripotent Stem Cells (PSC) in to Cardiomyocytes
- Video: How to Induce Pluripotent Stem Cells in to Definitive Endoderm
Mesenchymal Stem Cells (MSCs)
- Overview: Mesenchymal Stem Cells (MSCs)
- Guide: Mesenchymal Stem Cell Sourcebook
- Reference: Mesenchymal Stem Cell Assays and Applications
Neural Stem Cells (NSCs)
- Overview: Neural Stem Cells (NSCs)
- Overview: Gibco Human Neural Stem Cells
- Overview: Gibco Rat Fetal Neural Stem Cells
- Overview: Induced Pluripotent Stem Cells (iPSCs)
- Overview: Culture Systems and Reagents for Neural Differentiation
- Overview: Neural Stem Cell Culture Reagents for Cell Therapy Research
- Overview: Neural Cell Markers
- Protocol: Neural Cell Culture and Differentiation Protocols
- Protocol: Neural Stem Cell Protocols
- Selection: Which Neural Cell Culture Reagents are Right for You?
- Video: How to Use Gibco PSC Neural Induction Medium
Hematopoietic Stem Cells (HSCs)
- Overview: Hematopoietic Stem Cells (HSCs)
- Webinar: Stem Cell Webinars
- Browse our site for: Training, Events and Webinars
- Gibco Custom Media Configurator: Adjust many of our Gibco media formulations to create a custom cell culture medium that's just right for you.
- Cell Imaging Guide with Protocols (iPad™ device version): Find fluorescent dyes, reagents and protocols for cell biology related fluorescence microscopy applications.
- Fluorescence SpectraViewer: Plot and compare spectra, check the spectral compatibility for fluorophores and email the configuration to yourself in a clear printable format.
- Gibco Cell Culture Companion: Capture and store vital experiment information on your iPhone™ or iPad™ device in the lab as you count cells, perform cell culture calculations and generate growth curves. And when you're finished, you can export all data files to your Mac or PC.
- 3D Cell (iPad™ device version): Learn about the cell and all its structures, rotate the cell 360 degrees, watch live cell videos, and virtually stain your own cell with Invitrogen Molecular Probes dyes.
Custom Services
Custom Cell Culture Media Manufacturing Services
Every cell culture project is unique. We are committed to providing you with products and services customized to your research and production needs. Presenting the Gibco quality media you know and trust; customized to your specifications.
Gibco Custom Media Configurator
Adjust many of our Gibco media formulations to create a custom cell culture medium that's just right for you. Add and remove components, customize packaging options and choose QC tests. Customize Gibco cell culture media products from 1 L to 10,000 L.
Stem Cell CellModel Services
From human samples to induced pluripotent stem cell (iPSC) lines to screening, choose the steps we can do for you! Built on the stem cell innovations that we have introduced throughout the past decade, our CellModel Services help enable stem cell scientists to reach their desired outcomes faster. We offer choices at every stage of your stem cell research, innovative tools that make it easier for you to “do it yourself” and a custom services offering that utilizes our team of stem cell experts to deliver your desired results. Choose the combination of tools and services that best fits your lab resources and expertise.
For Research Use Only. Not for use in diagnostic procedures.