Search Thermo Fisher Scientific
Protein Gel 1D Electrophoresis Support
Explore the “Getting Started” and “Troubleshooting” sections for solutions to top inquiries and common problems. Browse through the “Guides and Tools” section to access comprehensive product-related support resources.
Getting Started
- Can I prepare my protein sample with the reducing agent and store it for future use?
- Can reducing agents other than DTT or BME be used to reduce proteins prior to electrophoresis? For example, what about TCEP (Tris Carboxy Ethyl Phosphene)?
- How are Bolt Bis-Tris Plus gels different from NuPAGE Bis-Tris gels?
- What is the composition of the NuPAGE LDS Sample Buffer and what is the recipe for the 4X formulation?
- What are the main advantages of NuPAGE gels over Novex Tris-Glycine gels?
Troubleshooting
- My protein bands in the outer lanes of the gel show a “smiling” effect. Can you please help me troubleshoot?
- I am getting wavy dye fronts while using the Bolt Bis-Tris Plus gels with the Bolt tank. Why is this?
- When I tried to remove the comb from my Bolt Bis-Tris Plus gel, I noticed the presence of extra acrylamide. What is the purpose?
- I ran my protein samples on a NuPAGE Bis-Tris gel, and I see a bubble in the center of the gel with significant band distortion particularly in the center lanes. What is the reason?
- I ran my protein samples on a Tris-Glycine gel and the samples stopped running in the bottom portion of the gel whereas the marker ran fine. Why did this happen?
Guides and Tools
Search our database for Product Documents: Manuals, Product Literature, Citations and References
Search our database for Quality and Safety Documents: MSDS, COA
General
- Overview: Protein Gel Electrophoresis
- Overview: Novex Precast Midi Gels
- Overview: Protein Separation by Protein Electrophoresis
- Learning Center: Protein Biology
- Protein Biology Resource Library (useful technical, application, and other learning resources including application notes and methods)
- Guide: Protein Analysis Handbook
- Selection: Find the Right Protein Gel for Your Experiments
- Selection: Protein Mini Gel Selection Guide
- Selection: Find our Equivalent to Competitor Gels
- Technical Note: Protein Separation Myths
- Technical Publication: Models of Protein Modification in Tris-Glycine and Neutral pH Bis-Tris Gels during Electrophoresis: Effect of Gel pH
- Video: Protein Analysis Tips and Tricks
- Video: Novex Precast Protein Gels – Beauty of a Straight Line
- Video: Novex™ Gel Cassettes – Easy, Simple Solutions that Make a Big Difference
- Video: Separate Proteins by SDS-PAGE for Western Blot
Novex Tris-Glycine Gels
- Overview: Novex Tris-Glycine Gels
- Gel Migration Chart: Novex Tris-Glycine Gels (for optimal results, protein bands should migrate within the yellow shaded areas)
- Selection: Choose the Right Novex Tris-Glycine Gel
NuPAGE Bis-Tris and NuPAGE Tris-Acetate Gels
- Overview: Novex NuPAGE SDS-PAGE Gel System
- Gel Migration Chart: NuPAGE Bis-Tris Gels (for optimal results, protein bands should migrate within the yellow shaded areas)
- Gel Migration Chart: NuPAGE Tris-Acetate Gels (for optimal results, protein bands should migrate within the yellow shaded areas)
- Selection: Choose the Right NuPAGE Bis-Tris Gel
- Selection: Choose the Right NuPAGE Tris-Acetate Gel
- Video: NuPAGE Novex Gel System
- Video: Meet The Inventor: NuPAGE System
Bolt Bis-Tris Plus Gels
- Overview: Bolt Bis-Tris Plus Gels
- Overview: Resources for Upgrading the Bolt Mini Gel Tank
- Gel Migration Chart: Bolt Bis-Tris Plus gels (for optimal results, protein bands should migrate within the yellow shaded areas)
- Selection: Bolt Gel Selection Guide
- Video: Operating the Mini Gel Tank
- Video: Replacing Cam Handles on Bolt Mini Gel Tank
Precise Protein Gels
- Overview: Precise Protein Gels
- Choose the Right Protein Mini Gel
- Choose the Right Novex Tris-Glycine Gel
- Choose the Right NuPAGE Bis-Tris Gel
- Choose the Right NuPAGE Tris-Acetate Gel
- Choose the Right BOLT Bis-Tris Plus Gel
- Choose the Right Precast Midi Gel
- NuPAGE Gel Conversion Guide: Easy and simple conversion from either a Tris-Glycine or Tricine gel to the appropriate NuPAGE gel and corresponding optimized buffer.
- Using gels from another vendor? Find the right equivalent gel from our company
- DailyCalcs Science Calculator: An app designed to calculate molarity, dilution, molecular weight, and more. Also includes a transfection calculator you can use to simplify transfection experimental setup.
Custom Services
GeneArt™ Cell Lines and Proteins Services
GeneArt™ Cell Lines and Proteins are a suite of service offerings designed to help facilitate protein expression in mammalian cell lines. We can help with every step of the process, including gene design, synthesis, cloning, protein production, and even stable cell generation. Alternatively, we can help with just the parts of the process you request.
Protein Expression and Production Services
Have us utilize our extensive suite of expression vectors and host systems to deliver the necessary protein yield for your application. We offer protein expression and production services in baculovirus and mammalian host systems, as well as protein purification and analysis services.
ProtoArray™ Services
Have our experienced scientists optimize your experimental design and maximize your desired outcome.
For Research Use Only. Not for use in diagnostic procedures.