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The Oncomine Focus Assay is a targeted, multi-biomarker assay that enables detection of hotspots, SNVs, indels, CNVs, and gene fusions from DNA and RNA in a single workflow. This comprehensive assay design is further enhanced with workflow automation, delivering insights quickly and helping accelerate your research towards that next companion diagnostic or therapy in the future.
Designed for clinical and translational cancer research, the Oncomine Focus Assay leverages proprietary Ion AmpliSeq technology to enable robust results, low input amounts, and analysis of low quality samples, and is compatible with FFPE fine needle aspirates and core needle biopsies.
Simultaneous detection of hundreds of variants across 52 genes relevant to solid tumors.
Hotspot genes | |
---|---|
AKT1
| IDH2 |
Copy number genes | |
---|---|
AKT1 | PIK3CA |
Gene fusions | |
---|---|
ABL1
| PDGFRA |
From FFPE samples including fine needle aspirates and needle biopsies, DNA and RNA is extracted, amplified, and templated into amplicon-based libraries ready to be sequenced.
Ion GeneStudio S5 Series enables high-throughput sequencing of 2M to 130M reads per run.
Seamless integration of our informatics solutions with our sequencing platforms enable quick and intuitive analysis and reporting.
From FFPE samples including fine needle aspirates and needle biopsies, DNA and RNA is extracted, amplified, and templated into amplicon-based libraries ready to be sequenced.
Oncomine Focus Assay is a targeted, multi-biomarker NGS assay that enables analysis on all classes of variants from DNA and RNA across 52 unique genes in a single workflow.
The Ion Chef System enables hands-free library templating of the Oncomine libraries into the Ion Chip for downstream sequencing.
The Ion GeneStudio S5 Series enable throughput capacity from 2 million to 130 million reads per run. Simply select the Ion Chip that provides the throughput and coverage needed for your application.
Plan, monitor, and track your runs all within a web interface while reviewing the quality and accuracy of your sequencing run, in addition to variant calling, detecting SNPs and indels.
Call and annotate SNPs/InDels, CNVs, and fusions. Interpret your DNA and RNA variants faster and more easily from semiconductor sequencing data using a web-enabled solution.
With Ion Torrent Oncomine Reporter, get clear and concise reports that link biomarkers to relevant evidence.
From FFPE samples including fine needle aspirates and needle biopsies, DNA and RNA is extracted, amplified, and templated into amplicon-based libraries ready to be sequenced.
Ion GeneStudio S5 Series enables high-throughput sequencing of 2M to 130M reads per run.
Seamless integration of our informatics solutions with our sequencing platforms enable quick and intuitive analysis and reporting.
From FFPE samples including fine needle aspirates and needle biopsies, DNA and RNA is extracted, amplified, and templated into amplicon-based libraries ready to be sequenced.
Oncomine Focus Assay is a targeted, multi-biomarker NGS assay that enables analysis on all classes of variants from DNA and RNA across 52 unique genes in a single workflow.
The Ion Chef System enables hands-free library templating of the Oncomine libraries into the Ion Chip for downstream sequencing.
The Ion GeneStudio S5 Series enable throughput capacity from 2 million to 130 million reads per run. Simply select the Ion Chip that provides the throughput and coverage needed for your application.
Plan, monitor, and track your runs all within a web interface while reviewing the quality and accuracy of your sequencing run, in addition to variant calling, detecting SNPs and indels.
Call and annotate SNPs/InDels, CNVs, and fusions. Interpret your DNA and RNA variants faster and more easily from semiconductor sequencing data using a web-enabled solution.
With Ion Torrent Oncomine Reporter, get clear and concise reports that link biomarkers to relevant evidence.
Targeted next-generation sequencing (NGS) is empowering laboratories of all sizes to confidently pioneer breakthroughs in cancer research. At the core of these breakthroughs are assays and panels designed to achieve highly sensitive results from minimal sample input—to help deliver information that may lead to the next companion diagnostic test or therapy in the future.
Clinical cancer research requires countless decisions. Of critical importance are the decisions you make about assay content. The Oncomine Focus Assay provides a robust assay where all genes provide actionable insights across a range of different cancer types.
Across variant types, the Oncomine Focus Assay achieves high sensitivity, specificity, and reproducibility using clinical research samples sequenced on the Ion GeneStudio S5 System.
Sensitivity | PPV (Specificity) | Reproducibility | |
SNVs | 98.77% | 99.63% | 99.6% |
Indels | 100% | 100% | 100% |
CNVs | 96.42% | 100% | 96.42% |
Gene fusion | 100% | 100% | 100% |
Overall | 98.70% | 99.67% | 99.01% |
Features enabled with Oncomine informatics:
For Research Use Only. Not for use in diagnostic procedures.