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Detailed analysis, characterization, and verification is critical to on-going success with any experiment. Learn more about workflow solutions for immuno-oncology research, from targeted genetic analysis through protein analysis.
Confirm and quantify genetic changes for a wide range of selected DNA targets that are key to your research.
Isolate the highest-quality genomic or viral DNA from a range of sample types for use in all common molecular biology applications.
Amplify DNA sequences for subsequent molecular biology applications.
Step 1: Amplify target DNA | Step 2: Analyze |
---|---|
Accurately analyze specific DNA targets.
Step 1: Prepare samples |
---|
We provide multiple solutions to isolate and purify DNA.
|
Step 2: Select assays |
We offer a variety of TaqMan Genotyping Assays for a number of real-time PCR analysis applications.
|
Step 3: Set up reactions |
Prepare a reaction plate using TaqMan Assays and TaqMan master mix, along with your DNA samples. No additional reagents are needed.
|
Step 4: Run PCR |
Amplify DNA and achieve allelic discrimination by running the PCR reaction on an Applied Biosystems real-time PCR system and/or thermal cycler. To better evaluate the accuracy of genotype calls, perform real-time time PCR and obtain genotyping data for every cycle. Or for higher throughput, run PCR on a thermal cycler and then perform an endpoint read on a real-time instrument.Thermocycler instruments
Real-time PCR instruments Compare instruments ›
|
Step 5: Analyze data |
Process and interpret qPCR data with intuitive software data analysis tools for a range of applications.
|
Verify DNA sequence rapidly using proven DNA sequencing technology.
Step 1: PCR amplification | Step 2: PCR clean-up |
---|---|
Step 3: Cycle sequencing | Step 4: Sequencing clean-up |
Step 5: Sequence | Step 6: Analyze data |
Sequence hundreds to thousands of targets at high depth.
Need help with your project? Request a quote ›
Step 1: Select targets | Step 2: Construct library and/or prepare template |
---|---|
Step 3: Sequence | Step 4: Analyze data |
Confirm and quantify RNA targets at the gene, exon, or noncoding RNA level.
Isolate the highest-quality cellular RNA, viral RNA, or miRNA from a range of sample types for direct use in all common molecular biology applications
Confirm and compare gene expression for small sample numbers by RT-PCR
Step 1: Synthesize and amplify cDNA | Step 2: Analyze data |
---|---|
Quantify gene expression with speed and accuracy.
Step 1: Isolate RNA |
---|
Isolate RNA using methods that preserve RNA integrity and expression profiles.
|
Step 2: Design and optimize primers |
|
Step 3: Reverse transcription |
Convert your RNA into cDNA, over a wide range of RNA concentrations.
|
Step 4: Amplify cDNA |
TaqMan real-time assays—Industry-leading, designed using our validated bioinformatics pipeline, and run with the same PCR protocol, eliminating the need for primer design or PCR optimization. Find the right assay for your research. Find assay now ›
|
Step 5: Run qPCR |
Instruments for real time PCR. Compare instruments ›
|
Step 6: Analyze |
Process and interpret qPCR data with intuitive software data analysis tools for a range of applications. |
Confirm thousands of biomarkers quickly and reproducibly.
Need help with your project? Request a quote ›
Step 1: Select targets | Step 2: Construct library and/or prepare template |
---|---|
Step 3: Sequence | Step 4: Analyze data |
Study the impact of external and internal perturbations on cellular phenotypes and behavior.
Step 1: Observe | Step 2: Count |
---|---|
Step 3: Visualize | Step 4: Analyze |
Microplate reading:
Flow cytometry:
Quantitative imaging: |
Analyze the identity, function, and level of expression of key proteins.
Step 1: Isolation and cleanup | Step 2: Separate |
---|---|
Step 3: Transfer | Step 4: Detect |
Step 5: Quantify | Step 6: Modify |
|
Confirm and quantify genetic changes for a wide range of selected DNA targets that are key to your research.
Isolate the highest-quality genomic or viral DNA from a range of sample types for use in all common molecular biology applications.
Amplify DNA sequences for subsequent molecular biology applications.
Step 1: Amplify target DNA | Step 2: Analyze |
---|---|
Accurately analyze specific DNA targets.
Step 1: Prepare samples |
---|
We provide multiple solutions to isolate and purify DNA.
|
Step 2: Select assays |
We offer a variety of TaqMan Genotyping Assays for a number of real-time PCR analysis applications.
|
Step 3: Set up reactions |
Prepare a reaction plate using TaqMan Assays and TaqMan master mix, along with your DNA samples. No additional reagents are needed.
|
Step 4: Run PCR |
Amplify DNA and achieve allelic discrimination by running the PCR reaction on an Applied Biosystems real-time PCR system and/or thermal cycler. To better evaluate the accuracy of genotype calls, perform real-time time PCR and obtain genotyping data for every cycle. Or for higher throughput, run PCR on a thermal cycler and then perform an endpoint read on a real-time instrument.Thermocycler instruments
Real-time PCR instruments Compare instruments ›
|
Step 5: Analyze data |
Process and interpret qPCR data with intuitive software data analysis tools for a range of applications.
|
Verify DNA sequence rapidly using proven DNA sequencing technology.
Step 1: PCR amplification | Step 2: PCR clean-up |
---|---|
Step 3: Cycle sequencing | Step 4: Sequencing clean-up |
Step 5: Sequence | Step 6: Analyze data |
Sequence hundreds to thousands of targets at high depth.
Need help with your project? Request a quote ›
Step 1: Select targets | Step 2: Construct library and/or prepare template |
---|---|
Step 3: Sequence | Step 4: Analyze data |
Confirm and quantify RNA targets at the gene, exon, or noncoding RNA level.
Isolate the highest-quality cellular RNA, viral RNA, or miRNA from a range of sample types for direct use in all common molecular biology applications
Confirm and compare gene expression for small sample numbers by RT-PCR
Step 1: Synthesize and amplify cDNA | Step 2: Analyze data |
---|---|
Quantify gene expression with speed and accuracy.
Step 1: Isolate RNA |
---|
Isolate RNA using methods that preserve RNA integrity and expression profiles.
|
Step 2: Design and optimize primers |
|
Step 3: Reverse transcription |
Convert your RNA into cDNA, over a wide range of RNA concentrations.
|
Step 4: Amplify cDNA |
TaqMan real-time assays—Industry-leading, designed using our validated bioinformatics pipeline, and run with the same PCR protocol, eliminating the need for primer design or PCR optimization. Find the right assay for your research. Find assay now ›
|
Step 5: Run qPCR |
Instruments for real time PCR. Compare instruments ›
|
Step 6: Analyze |
Process and interpret qPCR data with intuitive software data analysis tools for a range of applications. |
Confirm thousands of biomarkers quickly and reproducibly.
Need help with your project? Request a quote ›
Step 1: Select targets | Step 2: Construct library and/or prepare template |
---|---|
Step 3: Sequence | Step 4: Analyze data |
Study the impact of external and internal perturbations on cellular phenotypes and behavior.
Step 1: Observe | Step 2: Count |
---|---|
Step 3: Visualize | Step 4: Analyze |
Microplate reading:
Flow cytometry:
Quantitative imaging: |
Analyze the identity, function, and level of expression of key proteins.
Step 1: Isolation and cleanup | Step 2: Separate |
---|---|
Step 3: Transfer | Step 4: Detect |
Step 5: Quantify | Step 6: Modify |
|
For Research Use Only. Not for use in diagnostic procedures.