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There are many different apoptosis assays for flow cytometry because cell death cascades are complex and dynamic, underscoring the importance of a multi-parametric approach to the assessment of apoptosis. Since no single parameter defines programmed cell death, a combination of techniques is recommended for flow cytometry apoptosis detection.
We offer a wide variety of apoptosis assays for flow cytometry that detect changes in the plasma and mitochondrial membranes, caspase activity, nuclear condensation, and fragmentation as possible indicators of apoptosis. Mix and match our standalone reagents or choose from our selection of multi-parametric apoptosis assay kits that are designed to selectively differentiate apoptotic cells from living and necrotic cells in a single cell population.
Annexin V staining is used to detect the translocation of phosphatidylserine to the outer leaflet of the plasma membrane, a hallmark of apoptosis.
The activation of caspase enzymes is a distinctive feature of apoptosis.
Mitochondrial dysfunction is a feature of apoptosis. A wide variety of reagents are available to detect:
Apoptosis is characterized by changes in nuclear morphology such as DNA fragmentation.
Select flow cytometry apoptosis detection reagents based on the laser excitation source and common emission filters.
Emission filter | 379/28 nm | 440/40 nm |
Annexin V conjugates | ||
Membrane permeability & chromatin condensation |
Emission filter | 450/40 nm | 525/50 nm | 610/20 nm |
Annexin V conjugates | |||
Annexin V alternative (when the use of calcium containing buffers isn’t an option) | |||
Membrane permeability & chromatin condensation |
Emission filter | 530/30 nm | 574/26 nm | 695/40 nm | 780/60 nm |
Annexin V conjugates | ||||
Caspase activity | ||||
Membrane permeability & chromatin condensation | ||||
Dynamic changes in mitochondrial membrane potential | ||||
End point assays for mitochondrial membrane potential | ||||
Mitochondrial transition pore | ||||
TUNEL assays |
Emission filter | 585/16 nm | 620/15 nm |
Annexin V conjugates | ||
End point assays for mitochondrial membrane potential | ||
Mitochondrial superoxide production | ||
Mitochondrial calcium influx |
Emission filter | 585/16 nm | 620/15 nm |
Annexin V conjugates | ||
Dynamic changes in mitochondrial membrane potential | ||
End point assays for mitochondrial membrane potential |
Emission filter | 670/14 nm |
Annexin V conjugates | |
Membrane permeability & chromatin condensation | |
End point assays for mitochondrial membrane potential |
Emission filter | 379/28 nm | 440/40 nm |
Annexin V conjugates | ||
Membrane permeability & chromatin condensation |
Emission filter | 450/40 nm | 525/50 nm | 610/20 nm |
Annexin V conjugates | |||
Annexin V alternative (when the use of calcium containing buffers isn’t an option) | |||
Membrane permeability & chromatin condensation |
Emission filter | 530/30 nm | 574/26 nm | 695/40 nm | 780/60 nm |
Annexin V conjugates | ||||
Caspase activity | ||||
Membrane permeability & chromatin condensation | ||||
Dynamic changes in mitochondrial membrane potential | ||||
End point assays for mitochondrial membrane potential | ||||
Mitochondrial transition pore | ||||
TUNEL assays |
Emission filter | 585/16 nm | 620/15 nm |
Annexin V conjugates | ||
End point assays for mitochondrial membrane potential | ||
Mitochondrial superoxide production | ||
Mitochondrial calcium influx |
Emission filter | 585/16 nm | 620/15 nm |
Annexin V conjugates | ||
Dynamic changes in mitochondrial membrane potential | ||
End point assays for mitochondrial membrane potential |
Emission filter | 670/14 nm |
Annexin V conjugates | |
Membrane permeability & chromatin condensation | |
End point assays for mitochondrial membrane potential |
Cy™ is a trademark or registered trademark of GE Healthcare.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and are used under license.
For Research Use Only. Not for use in diagnostic procedures.