Histogram of 2 distinct peaks of viable/live cells and dead cell

Cell viability assays for flow cytometry are reliable methods to distinguish live and dead cell populations. Removing dead and dying cells from your flow cytometry data is critical to enable the accuracy of your results and analysis. Dead cells often give false positive results, as a compromised cell membrane allows for internalization and non-specific binding of many reagents.

Flow cytometry viability stains are available for use with fixable and non-fixable cell samples in a broad range of color options that are compatible with most instrument configurations.

LIVE/DEAD fixable viability dyesCell viability assaysLIVE/DEAD cell viability assaysBacterial viability and vitality assaysYeast viability and vitality assays
Amine-reactive dyesNucleic acid binding dyesEnzymatic substratesMammalian kits
Bacterial kits
Yeast kits		Bacterial kitsYeast kits
FixableNon-fixableNon-fixableNon-fixableNon-fixable; fixableNon-fixable
Cell-impermeantCell-impermeantCell-permeantCell-impermeant; cell-permeantCell-impermeant; Cell-permeantCell-impermeant; Cell-permeant
  • Reacts with cellular amine proteins
  • Based on membrane integrity
  • Reacts with cellular enzymes
  • Based on membrane integrity
  • Reacts with cellular enzymes
  • Indicator of metabolic activity
  • Based on membrane integrity
  • Indicator of reductase activity
  • Evaluates respiratory activity
  • Based on membrane integrity
  • Reacts with cellular enzymes
Dead cellsDead cellsLive cellsLive cells; Dead cellsLive cells; Dead cellsLive cells; Dead cells
UV—Near IRUV, Violet, Blue, Yellow, RedUV, Violet, Blue, YellowViolet, Blue, YellowViolet, Blue, YellowBlue, Yellow

See also:

Flow Cytometry Capabilities Guide

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Cy™ is a trademark or registered trademark of GE Healthcare.

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and are used under license.

For Research Use Only. Not for use in diagnostic procedures.

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