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Phospholipase * | Probes | Assay Principle | Detection Method |
---|---|---|---|
A1 | A10070, E10219, E10221 | Intramolecular self-quenching | Fluorescence increase at ~530 nm |
A1, A2 | B7701 | Intramolecular self-quenching | Fluorescence increase at ~515 nm ![]() |
A1, A2 | B3781, B3782 | Intramolecular excimer formation | Emission ratio 380/470 nm ![]() |
A1, A2 | A3880 | Free fatty acid sensor | Emission ratio 432/505 nm ![]() |
A2 | A10072, E10217, E10218 | Intramolecular fluorescence resonance energy transfer (FRET) | Fluorescence increase at ~515 nm or increase in emission ratio at 515/575 nm |
A2 | D23739 | Intramolecular self-quenching | Fluorescence increase at ~515 nm ![]() |
A2 | N3786, N3787 | Intermolecular self-quenching | Fluorescence increase at ~530 nm ![]() |
A2 | H361, H3809 | Intermolecular excimer formation | Emission ratio 380/470 nm ![]() |
A2 | D3803 | Release of a fluorescent fatty acid | TLC or fluorescence image scanner ![]() |
A2, C, D | D3771 | Formation of a fluorescent O-alkylglycerol derivative | TLC or HPLC ![]() |
A2, C, D | H361 | Quenching by a disulfide-polymerized lipid matrix | Fluorescence increase at ~380 nm ![]() |
C | A12218 | Peroxidase-linked detection of phosphocholine | Conversion of the nonfluorescent Amplex Red reagent to fluorescent resorufin ![]() |
C | E10215, E10216 | Release of dye-labeled diacylglycerol | Fluorescence increase at 516 nm, with potential interference from phospholipase A2 and phospholipase D activity |
D | A12219 | Peroxidase-linked detection of choline | Conversion of the nonfluorescent Amplex Red reagent to fluorescent resorufin ![]() |
PAP † | D3805 | Release of dye-labeled diacylglycerol | HPLC ![]() |
* Phospholipase specificity: A1, A2, C or D (see Probes for Lipid Metabolism and Signaling—Section 17.4 for cleavage specificities). † PAP = phosphatidic acid phosphohydrolase. |
For Research Use Only. Not for use in diagnostic procedures.