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Method of Plasma Membrane Breach | Size of Molecules Loaded (MW) * |
---|---|
Chemical | |
ATP ![]() | 1000 |
Cell-penetrating peptides ![]() | 150,000; (DNA) |
Influx pinocytic cell-loading reagent ![]() | 150,000 |
α-Toxin of Staphylococcus aureus![]() | 1000 |
Vehicle | |
Red blood cell fusion ![]() | 300,000 |
Cationic lipids ![]() | (DNA) |
Mechanical | |
Microinjection ![]() | 150,000 |
Whole-cell patch clamping ![]() | Very high |
Carbon nanotubes and nanowires ![]() | 150,000; (DNA) |
Scrape loading ![]() | 500,000; (DNA) |
Ultrasonication ![]() | 70,000; (DNA) |
Microprojectile bombardment ![]() | 10,000; (DNA) |
Glass beads ![]() | 150,000 |
Electrical | |
Electroporation ![]() | 150,000; (DNA) |
* Molecular weight (MW) of largest molecules reported loaded (DNA is listed separately if it has been successfully introduced by a technique). † We recommend the Neon® Transfection System (www.invitrogen.com/neon) for efficient electroporation of cultured cell lines, primary cells and stem cells. |
For Research Use Only. Not for use in diagnostic procedures.