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Hypercarb HPLC and UHPLC Columns
Your solution for difficult separations in both reversed phase and normal phase HPLC and LC/MS applications
Porous graphitic carbon (PGC, Hypercarb™) has unique properties as a stationary phase in high-performance liquid chromatography (HPLC). Its chemical surface properties distinguish it from more conventional LC packings such as bonded-silica gels and polymers. PGC behaves as a strongly retentive alkyl-bonded silica gel for non-polar analytes.
However, its retention and selectivity behavior towards polar and structurally-related compounds is very different from conventional C18 columns.
Features of Hypercarb columns
Thermo Scientific™ Hypercarb Porous Graphitic Carbon LC Columns offer:
- Exceptional retention of very polar analytes
- Separation for structurally-related substances
- pH stablility from 0 to 14, and are not affected by aggressive mobile phases
- Ideal solution for high-temperature applications
Physical properties of PGC
| Property | To meet requirement of | |
| Particle shape: | Spherical | No micropores |
| Specific surface area: | 120 m2/g | Retention linearity and loading capacity |
| Median pore diameter: | 250 Å | Mass transfer for wide range of analyte shapes and sizes |
| Pore volume: | 0.7 m3/g | |
| Mean particle diameters: | 3 µm, 5 µm | Analytical HPLC columns |
| 7 µm | Preparative HPLC columns | |
| 30 µm | SPE applications | |
| Porosity: | 75% | Mass transfer within particles |
| % C: | 100% | Chemical stability |
| Mechanical strength: | <400=""> | Operational particle stability; pressure gradients in packing process |
Hypercarb columns literature
2022/2023 HPLC/UHPLC Columns Catalog
Access the latest interactive version of our Chromatography Consumables & Columns Catalog. Our enhanced online version features easier access to product information, key application notes and brand-new selector tools.
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