Red fluorescent protein (RFP) is a biological marker used for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression in vivo. RFP comes in many forms including tdTomato, mCherry, mStrawberry, and DsRed and produces a bright red fluorescence when exposed to blue light.Anti-RFP antibodies provide a dependable method for the detection and purification of tagged target proteins without a protein-specific antibody or probe. They provide a convenient method for visualizing RFP, especially, when amplification of the fluorescent protein of interest is necessary to overcome a weak or degraded signal. Invitrogen RFP tag antibodies reliably detect multiple forms of RFP on tagged proteins and each antibody is tested for use in a variety of applications.

See all anti-RFP tag antibodies


Applications

Invitrogen anti-RFP antibodies are designed to dependably detect RFP in various applications.

Immunofluorescence using anti-RFP antibody to stain cells

Immunofluorescence using an anti-RFP antibody. HeLa cells were incubated with Organelle Lights Mito-RFP (O36229) and Organelle Lights Golgi-GFP (O10098). Following fixation and permeabilization, cells were stained with Alexa Fluor 350 phalloidin (Cat. No. A22281) and RFP Polyclonal Antibody (Cat. No. R10367), the latter subsequently visualized with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 (Cat. No. A-21245, red fluorescence) and imaged. No cross-reactivity is observed for the anti-RFP antibody with GFP (green-fluorescence, perinuclear region).

Flow cytometry analysis of red fluorescent protein (RFP) using a specific antibody. Fixed and permeabilized HEK293 cells were transduced by CellLight Tubulin-RFP, BacMam 2.0 (Cat. No. C10614, right peak, orange), and quenched by an RFP Recombinant Polyclonal Antibody (Cat. No. 710530, middle peak, filled). Non-transduced cells are shown in blue (left peak).

Resources

For Research Use Only. Not for use in diagnostic procedures.