Beta galactosidase (beta-Gal or β-Gal) is an enzyme that catalyzes the hydrolysis of beta galactosides into monosaccharides and is an essential enzyme for humans. Deficiencies in the beta galactosidase protein can result in galactosialidosis or Morquio B syndrome. In E. coli, the lacZ gene, which encodes for beta galactosidase, is part of the lac operon that is activated in the presence of lactose when the glucose level is low. Beta galactosidase is widely used as a reporter marker to monitor gene expression. It is also used in the blue/white screen for detecting recombinant bacteria. In addition, beta galactosidase is highly expressed in the lysosomes of senescent cells, so it is frequently used as a biomarker to detect aging and senescent cells. Invitrogen beta galactosidase antibodies are designed to reliably detect the enzyme in various applications.

See all beta-galactosidase antibodies


Applications

Invitrogen beta galactosidase antibodies are designed to dependably detect beta-galactosidase in various applications.

Immunofluorescence analysis using beta galactosidase monoclonal antibody

Immunofluorescent analysis of E. coli beta-galactosidase (green) in pCMV-LacZ transfected HeLa cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature. Cells were blocked with 1% Blocker BSA (Cat. No. 37525) for 15 minutes at room temperature. Cells were probed with a beta Galactosidase Monoclonal Antibody (DC1 4C7) (Cat. No. MA1-152) at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with a Goat anti-Mouse IgG (H+L) Secondary Antibody, DyLight 488 (Cat. No. 35502). Nuclei (blue) were stained with Hoechst 33342 dye (Cat. No. 62249). Images were taken on a Thermo Scientific ToxInsight instrument at 20x magnification.

Resources

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