The endoplasmic reticulum (ER) is an organelle consisting of a network of cisternae, tubules, and vesicles which are continuous with the outer membrane of the nuclear envelope. The endoplasmic reticulum occurs in two forms: smooth endoplasmic reticulum and rough endoplasmic reticulum. The smooth ER varies in specific function depending on the cell type, but it is generally involved in lipid and steroid synthesis, detoxification, and calcium homeostasis. The rough ER is bound by ribosomes, which are the sites of protein synthesis. These proteins collect in the endoplasmic reticulum for transport throughout the cell.

Endoplasmic reticulum marker antibodies can aid in the study of the structure and function of the ER. Endoplasmic reticulum marker antibodies can also help elucidate the role or roles a protein may play in a number of tasks that are centered in or influenced by the ER. Endoplasmic reticulum marker antibodies are particularly useful in labeling fixed cells. Invitrogen endoplasmic reticulum marker antibodies are designed to dependably detect the key ER targets. Each antibody is validated for use in various applications. Key endoplasmic reticulum marker targets include:

Endoplasmic reticulum marker antibody targets

Applications

Quality Invitrogen endoplasmic reticulum marker antibodies are available for your research needs.

Immunohistochemistry performed on normal deparaffinized human tonsil tissue tissues

Immunohistochemistry detection of SERCA2 ATPase performed on normal deparaffinized human tonsil tissue tissues. To expose target proteins, heat-induced antigen retrieval was performed using 10 mM sodium citrate (pH 6.0) buffer, microwaved for 8–15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing SERCA2 ATPase (Cat. No. MA3-919) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Immunofluorescent analysis of calreticulin

Immunofluorescent analysis of Phalloidin (green) and Calreticulin (red) in U2OS cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked with 2% BSA (Cat. No. 37525) in PBS + 0.1% Triton X-100 for 30 minutes at room temperature. Cells were probed with a Calreticulin polyclonal antibody (Cat. No. PA3-900) at a dilution of 1:75 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 633 goat anti-rabbit IgG secondary antibody (Cat. No. 35562) at a dilution of 1:250 for 30 minutes at room temperature. Actin was stained with DyLight 488 Phalloidin (Cat. No. 21833) at a dilution of 1:300 (1 unit/mL final concentration) and nuclei (blue) were stained with Hoechst (Cat. No. 62249) at a concentration of 1 µg/mL for 30 minutes. Images were taken on a Thermo Scientific ArrayScan VTI at 20X magnification.

Resources

For Research Use Only. Not for use in diagnostic procedures.