The Golgi complex is a key organelle in the secretory pathway of the cell. It receives proteins from the ER (endoplasmic reticulum) and processes and sorts them for further transport to lysosomes, the plasma membrane, or back to the ER. The Golgi complex is composed of flattened stacks of membranes (cisternae) organized into four functionally distinct areas: the cis-Golgi network, the medial cisternae, the trans cisternae, and the trans-Golgi network. Each region of the Golgi complex performs distinct processing and sorting events as proteins enter at the cis face and exit from the concave trans face. The Golgi complex also prepares other macromolecules such as lipids and carbohydrates for secretion from the cell or for use by other organelles.

Golgi marker antibodies can aid in the study of the dynamics and morphology of each part of the Golgi complex. These antibodies can also help elucidate the roles a protein may play in a number of tasks that are centered in or influenced by the Golgi. Golgi marker antibodies are designed to dependable detect the key golgi targets. Each antibody is validated for use in various applications. Key Golgi marker targets include:

Golgi marker antibody targets

Applications

Quality Invitrogen golgi marker antibodies are available for a variety of research needs.

Immunofluorescence analysis of Golgi bodies in muntjac skin cells

Muntjac skin cell labeled in three different colors. Golgi bodies in a muntjac skin cell were labeled with anti-Golgin-97 monoclonal antibody (Cat. No. A-21270) and visualized with green-fluorescent Alexa Fluor 488 Goat Anti-Mouse IgG1 antibody (Cat. No. A-21121). Filamentous actin was labeled with Alexa Fluor 680 phalloidin (Cat. No. A22286, pseudocolored purple). Nuclei were stained with blue-fluorescent DAPI (Cat. No. D1306, D3571, D21490).

Flow cytometry analysis of GM130

Flow cytometry analysis of GM130 on HeLa cells. Cells were fixed, permeabilized and stained with a GM130 rabbit polyclonal antibody (Cat. No. PA1-077, green histogram) or a rabbit IgG isotype control (Cat. No. MA5-16384, black histogram) at a dilution of 10 µg/mL. After incubation for 1 hour on ice, the cells were labeled with a Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor 647 conjugate (Cat. No. A27040) at a dilution of 1:50 for 1 hour on ice. A representative 10,000 cells were acquired and analyzed for each sample.

Immunohistochemical analysis of GRASP65

Immunohistochemistry analysis of GRASP65. Showing staining in the cytoplasm of paraffin-treated rat kidney tissue (right) compared with a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 minutes. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 minutes at room temperature, washed with ddH2O and PBS, and then probed with a GRASP65 polyclonal antibody (Cat. No. PA3-910) diluted by 3% BSA-PBS at a dilution of 1:200 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Resources

For Research Use Only. Not for use in diagnostic procedures.