We offer primary antibodies for studying various targets in cancer research. Whether your research is focused on metastasis, proliferative signaling, apoptosis, autophagy, metabolism, inflammation, tumor suppressors or any other cancer-related research area, we have a broad selection of research antibodies to help ensure your success. Invitrogen cancer research antibodies are designed to dependably detect the key cancer targets. Each antibody is validated for use in various applications. Some featured cancer research antibody targets and categories include:


Applications

Invitrogen cancer research antibodies are designed to dependably detect the key cancer targets and are validated for use in various applications.

Immunofluorescence staining of MAPK11

Immunofluorescence staining of MAPK11 in Hela cells. Cells were fixed with 4% PFA, permeabilized with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with p38 MAPK beta Polyclonal Antibody (Cat. No. PA5-81217) (dilution ratio: 1:1000) at 4° C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus. Immunofluorescence staining of MAPK11 in Hela cells. Cells were fixed with 4% PFA, permeabilized with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with p38 MAPK beta Polyclonal Antibody (Cat. No. PA5-81217) (dilution ratio: 1:1000) at 4° C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.

Immunohistochemistry analysis of AKT/PKB (pS473)

Immunohistochemistry analysis of AKT/PKB (pS473) showing staining in the cytoplasm and nucleus of paraffin-embedded human prostate carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Phospho-AKT1 (Ser473) Monoclonal Antibody (14-6) (Cat. No. 44-621G) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Resources

For Research Use Only. Not for use in diagnostic procedures.