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Super Bright 780 Antibody Conjugates
Comparable to Brilliant Violet 786 conjugates
Invitrogen eBioscience Super Bright 780 antibody conjugates for flow cytometry provide:
- Reduced spillover into other violet channels
- Options in marker and clone selection for violet laser–excitable antibody conjugates
- Compatibility with standard intracellular buffers, viability stains, compensation beads, and other antibodies
Multiplexing with Super Bright 780 antibody conjugates
Figure 1. Fluorescence intensity comparison of Super Bright 780 conjugates and Brilliant Violet™ 786 conjugates. (A) Mouse splenocytes stained with anti-CD4 conjugated to Super Bright 780 (red, Cat. No. 78-0042-80) or Brilliant Violet 786 conjugate (gray), at the same concentration of antibody. (B) Human peripheral blood cells stained with anti-CD8a conjugated to Super Bright 780 (red, Cat. No. 78-0088-41) or Brilliant Violet™ 786 conjugate (gray), using the same concentration of antibody.
Figure 2. Fluorescence intensity comparison of Super Bright 780 conjugates and Brilliant Violet™ 786 conjugates. Human peripheral blood cells stained with anti-CD19 conjugated to (A) Brilliant Violet™ 786 or (B) Super Bright 780 (Cat. No. 78-0198-41) at the same concentration of antibody.
Figure 3. Fluorescence intensity comparison of Super Bright 780 conjugates to eFluor 450 and PE conjugates. Mouse splenocytes cells stained with anti-CD3e conjugated to (A) eFluor 450 (Cat. No. 48-0621-80), (B) PE (Cat. No. 12-0621-81) or (C) Super Bright 780 (Cat. No. 78-0621-80).
| Multiplexing compatibility | Buffer | Multiplexing considerations |
|---|---|---|
| Multiplexing with 1 Super Bright antibody conjugate | Standard buffers applicable | No special buffer required when only one Super Bright antibody conjugate is used in a panel |
| Multiplexing with 2 or more Super Bright antibody conjugates | Super Bright Staining Buffer | Special staining buffer is required prior to addition of Super Bright conjugates to reduce non-specific dye-dye interactions |
| Multiplexing with 1 or more Brilliant Violet™ antibody conjugates | Super Bright Staining Buffer | Special staining buffer is required to reduce non-specific dye-dye interactions. Use of the Super Bright Staining Buffer is recommended, but the similar Brilliant Stain Buffer can be substituted |
| Viability stain options | Product | Multiplexing considerations |
|---|---|---|
| Fixable | LIVE/DEAD fixable dead cell stain kits | No compatibility concerns |
| Non-fixable | SYTOX non-fixable dead cell stains Ready Flow Ready-to-use viability reagents | No compatibility concerns Compatible with all Ready Flow reagents for viability |
| Product | Multiplexing considerations |
|---|---|
| UltraComp eBeads microspheres | UltraComp eBeads microspheres are compatible but OneComp eBeads are not compatible with violet lasers; the AbC Total Antibody Compensation Bead Kit is also compatible with the Super Bright antibody conjugates. |
| Staining Target | Product | Multiplexing considerations |
|---|---|---|
| Cytosolic staining (cytokines) | Intracellular Fixation & Permeabilization Buffer Set | No compatibility concerns |
| Nuclear staining (transcription factors) | Foxp3/Transcription Factor Staining Buffer Set | No compatibility concerns |
Figure 4. Staining performance and post-fixation stability. Human peripheral blood cells stained with CD8a antibody conjugated to Super Bright 780 (Cat. No. 78-0088-41) and analyzed immediately (red) or subjected to different fixation buffers per the recommended protocol: (A) eBioscience Foxp3 / Transcription Factor Staining Buffer Set (Cat. No. 00-5523-00), (B) eBioscience IC Fixation Buffer (Cat. No. 00-8222-49), or (C) eBioscience IC Fixation Buffer followed by 30 minutes in 90% methanol and were analyzed at 30 min (blue), overnight (orange) or after 3 days (green).