Brilliant Violet™ 605 Dye

When using two or more Super Bright, Brilliant Violet, Brilliant Ultra Violet™, or other polymer dye- conjugated antibodies in a staining panel, we recommend that you use Super Bright Complete Staining Buffer (Cat. No. SB-4401-42) or Brilliant Stain Buffer (Cat. No. 00-4409-42) to minimize any non- specific polymer interactions.

BV605 dye dashboard

Initial brightness		The violet laser-excitable BV605 dye is optimized for use both cell surface and intracellular flow applications. Can be used for spectral flow cytometry applications.
Photostability in buffer

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	405	610/20	407	605
	Laser line	Filter	Excitation max	Emission max

We offer BV605 dye conjugated to primary antibodies for use in flow cytometry.

Antibody conjugates

• Primary antibodies
• Control antibodies for flow cytometry

Brilliant Stain Buffer
Super Bright Staining Buffer

Figure 1. Excitation and emission of Brilliant Violet™ 605 dye (BV605).

Figure 2. Spectral signature of BV605 dye. Data acquired on a 5-laser Cytek Aurora and normal human peripheral blood cells stained with clone RPA-T8 (hCD8) conjugated to Brilliant Violet™ 605 dye (Cat. No. 406-0049-41) were used for analysis.

Figure 3. Normal human peripheral blood cells were stained with CD3 Monoclonal Antibody, FITC (Cat. No.11-0037-42) and Mouse IgG1 kappa Isotype Control, Brilliant Violet™ 605 dye (Cat. No. 406-4714-81) (left) or CD4 Monoclonal Antibody, Brilliant Violet™ 605 dye (right). Viable cells in the lymphocyte gate were used for analysis, as determined by 7-AAD (Cat. No. 00-6993-50).

BRILLIANT ULTRA VIOLET™ is a trademark or registered trademark of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.