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NovaFluor Red 685 Dye
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| 4 | 640 | 695/40 | 637 | 685 | (in buffer) 5 | flow cytometry |
Invitrogen NovaFluor Red 685 dye is ~4 times brighter than APC-Cyanine 5.5 and Alexa Fluor 680 dye. In complex multicolor flow cytometry panels, NovaFluor Red 685 dye can be used to fill the spectral gap between Alexa Fluor 647 and Alexa Fluor 700 dyes.
The macromolecule-based NovaFluor Red 685 dye produces highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C. Use NovaFluor dyes with CellBlox Plus Blocking Buffer to reduce background and to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.
We offer NovaFluor dyes conjugated to primary antibodies for use in flow cytometry, as well as NovaFluor Antibody Conjugation Kits, NovaFluor CD4 Label Characterization Kits, and custom conjugation services.
- Narrow emission spectrum and minimal cross-laser excitation for better resolution
- Bright fluorescence allows it to be paired with medium-to-low abundance antigens
- Can be used to fill the spectra gap between Alexa Fluor 647 and Alexa Fluor 700 dyes
- Can be used in a flow cytometry panel with NovaFluor Red 660 (or APC-Alexa Fluor 647) and Alexa Fluor 700 dyes
- Produces highly stable fluorescence and is compatible with all buffers tested
Click image to enlarge
Spectral signature of NovaFluor Red 685 dye. Normal human peripheral blood cells stained with CD4 Monoclonal Antibody (SK3 (SK-3)), NovaFluor Red 685 were used for analysis. Data was acquired on a 5-laser Cytek Aurora system.
Staining of human peripheral blood cells with NovaFluor Red 685 conjugated antibody. Normal human peripheral blood cells (PBMCs) were unstained (left) or stained with CD16 Monoclonal Antibody, NovaFluor Red 685 (right). Data was acquired in the R3 channel on a 5-laser Cytek Aurora system, and singlet cells were used for analysis.
Additional resources
Flow cytometry protocols handbook
Protocols that fit your needs in flow cytometry ranging from sample preparation to numerous cell stimulation conditions, staining, immunophenotyping, and data analysis strategies.
Fluorophore and reagent selection guide for flow cytometry
A handy reference poster featuring the broad range of our available dyes and labeling reagents.
Imaging protocol handbook
Protocols that fit your needs in imaging ranging from sample and assay preparation to staining, labeling, and data analysis strategies.
Fluorophore and reagent selection guide for cell imaging
A tool for selecting the optimal fluorescent dyes for your Invitrogen EVOS cell imaging systems.
Cy™ is a trademark or registered trademark of GE Healthcare.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and are used under license.
For Research Use Only. Not for use in diagnostic procedures.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Brilliant Ultra Violet™ and Brilliant Violet™ are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and is used under license.
For Research Use Only. Not for use in diagnostic procedures.