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Análisis de fragmentos
Aplicaciones de análisis de fragmentos para el analizador genético
La otra mitad del analizador de genética de Applied
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El analizador genético de electroforesis capilar (CE) es más potente de lo que se pueda imaginar. Esta plataforma permite el uso de aplicaciones de secuenciación y análisis de fragmentos. El análisis de fragmentos de ADN permite el uso de infinidad de aplicaciones desde el genotipado hasta la identificación de bacterias o desde la identificación de vegetales hasta la determinación de perfiles de expresión génica. Descubra cómo puede adaptar estas aplicaciones para ampliar las posibilidades de su investigación.
Go beyond sequencing
Analysis of DNA fragments enables a variety of applications, from cell line
authentication to detection of aneuploidy. While sequencing techniques, such as next-generation sequencing (NGS) also enable these applications, researchers choose fragment analysis for features such as detection of multi-repeat regions, a faster turnaround time, and a higher sensitivity and resolution.
Fragment analysis is also more cost-effective due to its multiplexing capability; alleles for overlapping loci are distinguished by labeling locus-specific primers with different colored dyes, enabling more than 20 loci to be analyzed in a single run.
How Does Fragment Analysis Work?
The Other Half of Your Applied Biosystems Genetic Analyzer
Brochure: DNA fragment analysis by capillary electrophoresis
Learn about our comprehensive solutions for each step of your fragment analysis workflow.
On-demand Webinar: Utility of Fragment Analysis in the Era of NGS-based Clinical Research
Speakers:
Somak Roy, MD, Cincinnati Children’s Hospital Medical Center
Steve Jackson, PhD, Thermo Fisher Scientific
Applications and methods
Misidentification of cell lines produces misleading results, confusion, and added costs to research. Many journals and funding agencies now require researchers to ascertain that the cell lines they use are authentic. Fragment analysis can provide a simple, inexpensive, and highly specific genetic “fingerprint” of a cell line.
Sanger sequencing is the gold standard for sequencing single genes, confirming gene variants, detecting repeat sequences, copy number variation, and single nucleotide changes. Perfect for smaller-scale projects and for confirmation of NGS results, Sanger sequencing can complement your laboratory's techniques and lead to efficient SARS-CoV-2 research.
Microsatellite markers are polymorphic DNA loci containing repeated nucleotide sequences, typically 2 to 7 nucleotides per repeat unit. The length of the repeated unit is the same for the majority of the repeats within an individual microsatellite locus, but the number of repeats for a specific locus may differ, resulting in alleles of varying length, which can be analyzed with fragment analysis by capillary electrophoresis.
The CRISPR-Cas9 system, the easiest, most precise, and most widely adopted genome editing technology, is based on the components of a simple bacterial immune system. In any genome editing experiment, the repair process is not completely efficient or accurate. Fragment analysis can be used to screen primary transformed cultures to determine editing efficiency.
Relative fluorescent quantitation or quantitative fluorescence PCR (QF-PCR) is a technique used in a variety of fragment analysis applications that requires accurate peak height or peak area comparisons across multiple samples. Research applications that utilize this technique include evaluating loss of heterozygosity (LOH), aneuploidy assays, and detecting large chromsomal deletions.
Single-nucleotide polymorphism (SNP) genotyping is a measure of genetic variation. SNP genotyping is used to research differences in genetic traits, susceptibility to disease, and response to drug therapies. A robust tool for SNP analysis is the Applied Biosystems™ SNaPshot™ Multiplex Kit. The kit allows multiplexing during single base extensions of up to 10 primer–template combinations in a single-tube, single-capillary format.
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Relative fluorescent quantitation or quantitative fluorescence PCR (QF-PCR) is a technique used in a variety of fragment analysis applications that requires accurate peak height or peak area comparisons across multiple samples. Research applications that utilize this technique include evaluating loss of heterozygosity (LOH), aneuploidy assays, and detecting large chromsomal deletions.
Bacterial Artificial Chromosomes (BAC) have been developed to accommodate much larger pieces of DNA than plasmids can. They can be used to sequence an organism’s genome, and to model genetic diseases.
SSCP analysis detects sequence variations (single-point mutations and other small-scale changes). It is a widely used screening method that allows for the identification of different genomic variants in a large number of samples and in a broad range of organisms, from microorganisms to humans.
DNA sequence polymorphisms display different migration profiles from wild-type fragment patterns when DNA is digested with restriction fragments and separated by size using electrophoresis. RFLP analysis is useful in identifying where a specific gene for a disease lies on a chromosome and determine individuals who might be at risk for the disease or a carrier of the mutated gene.
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 | Fragment Analysis FundamentalsDesde la preparación de muestras hasta el análisis de valores máximos, descubra los aspectos básicos del análisis de fragmentos |  | Fragment Analysis ApplicationsEl análisis de fragmentos le ofrece los resultados que necesita. Busque la aplicación que responderá a su pregunta. |
 | SNP Genoyting by Fragment-AnalysisAnalice hasta 10 SNP mediante una sola reacción con independencia de la posición del cromosoma con el sistema múltiple SNaPshot®. |  | Microsatellite Marker AnalysisUse marcadores de microsatélites sumamente informativos para los estudios de cartografía de acoplamiento, los estudios de asociación y la identificación de organismos. |
 | Quantitative Fluorescence PCRAproveche las funciones de comparación de alturas múltiples y máximas de las aplicaciones QM-PSF, QF-PCR y MLPA para detectar grandes reordenaciones cromosómicas. |  | AFLP, RFLP, SSCP & BAC FingerprintingDetecte la variación genética de un producto de PCR o un genoma mediante métodos de determinación de huellas de ADN como AFLP, RFLP y SSCP. |
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