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Ion AmpliSeq Custom Next-Generation Sequencing (NGS) DNA Panels
Targeted DNA panels for any genome
If the Ion AmpliSeq ready-to-use panels don't match your gene targets, then simply select a subset of the targets or enter your specific genomic content online and we will design and create customized Ion AmpliSeq NGS panels just for you.
Creating and ordering custom gene panels is easy with the Ion AmpliSeq Designer—a free, online assay design tool that connects you directly to our assay design pipeline. Panels can be designed against a number of preloaded genomes, or for any other organism by uploading your own FASTA file reference sequence.
Preloaded reference genomes available
Custom panels are delivered in tubes of prepooled, multiplexed primers in ready-to-use concentrations. You can add 384-well plates to your order, in which each well contains individual primer pairs in concentrated format. For custom panels with ≤96 primer pairs, you will receive 2,250 total reactions. For custom panels with >96 primer pairs, you will receive 9,000 total reactions.
Ion AmpliSeq Designer performance specifications
| Specifications* | Current value |
|---|---|
| Input DNA amount per primer pool | As little as 1 ng |
| Available reference genomes | Cow, chicken, human, maize, mouse, pig, rice, sheep, soybean, tomato |
| Custom references | Upload any reference genome to a password-protected cloud space |
| Primer Pairs per pool | 12 to 24,000 |
| Target size | 1 kb to 5 Mb |
| Target design rate** | >90% |
| Coverage uniformity† | >85% |
** Target design rate—bases covered by the insert region of amplicons out of total target bases submitted.
† Typical coverage uniformity—bases covered at >20% of the mean coverage.