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B Cell Receptor (BCR) Immunoglobulin Heavy (IGH) Chain Sequencing
The Ion Torrent portfolio of B cell receptor (BCR) assays targets the immunoglobulin heavy (IGH) chain for next-generation sequencing (NGS). Fast and affordable B cell repertoire analysis is achieved with little DNA or RNA input, using the sequencing accuracy and sensitivity of the Ion GeneStudio S5 series systems.
The complete Ion Torrent sample to analysis workflow enables deeper insights through targeted sequencing.
- Study B cell development
- Discover biomarkers that may potentially be predictive for future immunotherapies or helpful in the evaluation of vaccine efficacy research
- Quantify somatic hypermutations for possible insights in malignant lymphocyte transformation
Explore your area of interest
Oncomine BCR IGH LR Assay
The Ion Torrent Oncomine BCR IGH LR Assay uses the long-read sequencing capabilities of Ion Torrent technology to interrogate all complementarity determining regions (CDR1, CDR2, and CDR3) of the BCR IGH chain using low RNA sample input.
- Repertoire analysis with flexible input requirements ranging from 10 ng to 2 µg
- Assess clonal expansion and evaluate clonal evolution
- Quantify somatic hypermutation for clonal lineage analysis; potential insights into B cell development and malignant transformation
- Evaluate isotype abundance
Oncomine BCR IGH SR Assay
The Ion Torrent Oncomine BCR IGH-SR Assay allows you to interrogate the CDR3 region of the BCR IGH chain from human samples using either DNA or RNA as input. Targeting the diverse CDR3 region for B cells enables deeper insights in studying vaccine response, learning more about the cancer immunity cycle and research for evaluating measurable residual disease (MRD).
- Repertoire analysis with either DNA or RNA sample input
- Identify low frequency clones—detect B cell malignancies with 10-6 limit of detection
- Study isotype representation with accurate sequencing data
Ion AmpliSeq Mouse BCR IGH SR Assay
Transform your mouse model studies with targeted sequencing of the CDR3 region of the BCR IGH. Using either DNA or RNA sample input, the accuracy and sensitivity of the panel paired with an Ion GeneStudio S5 system enables a complete workflow that allows you to explore your data in as little as two days.
- Assess clonality state using either DNA or RNA sample input
- Monitor disease state and vaccine efficacy in your mouse models
- Complete sample to insight workflow for fast and easy analysis
Workflow for the Ion Torrent BCR kits
From sample to insights in as little as 48 hours.
- Whole blood
- Bone marrow
- Peripheral blood leukocytes (PBLs)
- Peripheral blood mononuclear cells (PBMC)
- Fresh frozen (FF)
- Formalin-fixed, paraffin-embedded (FFPE) tissue, for SR assays only
- Libraries require 10 ng to 2 μg DNA input or RNA input
- Sequence samples using an Ion GeneStudio S5 System
- Perform downstream data analysis with Ion Reporter Software 5.12 and up
- Whole blood
- Bone marrow
- Peripheral blood leukocytes (PBLs)
- Peripheral blood mononuclear cells (PBMC)
- Fresh frozen (FF)
- Formalin-fixed, paraffin-embedded (FFPE) tissue, for SR assays only
- Libraries require 10 ng to 2 μg DNA input or RNA input
- Sequence samples using an Ion GeneStudio S5 System
- Perform downstream data analysis with Ion Reporter Software 5.12 and up
Ion GeneStudio S5 series systems
Adopting NGS in your lab is now simpler than ever before. The Ion GeneStudio S5 series next-generation sequencing systems enable the simplest targeted sequencing workflow for your lab with superior speed and affordability. Focus your research efforts on applications that have the potential to be impactful. These NGS systems have a modular build, so your lab has the flexibility to do multiple targeted sequencing applications on a single system.