March 2021

Rapid detection of Salmonella serovars in single PCR test

Multi-target approach cuts costs, reduces delays, and increases confidence

Salmonella is a global threat to the public, business, and health. The Centers for Disease Control (CDC) estimates that the bacteria cause about 1.35 million infections, 26,500 hospitalizations, and 420 deaths in the United States every year.1 The estimated annual cost to healthcare systems in the United States is more than $3.6 billion.2

It’s a problem that regulators are increasingly attempting to tackle. Both the US and Europe have introduced tracking requirements for the two serotypes most responsible for human infection, Salmonella Enteritidis and Salmonella Typhimurium, in poultry and other high-risk areas, such as pork and eggs.

To date, Salmonella testing has involved confirming the presence of the bacteria, then carrying out further tests to identify the serotype before taking the relevant corrective action. This is often an expensive, time-consuming journey that delays workflows, impacts timelines, and introduces risk into the food chain.

Food producers are more than aware of their moral and regulatory duty to protect public health and are looking for laboratories that can provide a targeted, streamlined approach.

Thermo Fisher Scientific’s new Thermo Scientific™ RapidFinder™Salmonella species, Typhimurium and Enteritidis Multiplex Flex Kit workflow is a fast path to actionable answers. It is the only validated, certified system that can identify Salmonella species as well as differentiate between serovars Enteritidis and Typhimurium, and it can return next-day results. In short, it streamlines testing workflow and drastically reduces the wait time from testing to intervention or product release.

Compared with running separate PCR tests, the RapidFinder Salmonella Multiplex Kit workflow can reduce the cost of consumable goods by up to 50 percent, significantly lower labor costs – and increase confidence in food safety.

The system, which is internationally validated for detecting the three targets in primary production samples, ground turkey, pork and poultry meat, shell eggs, and production environment samples, offers in-house and contract testing laboratories:

  • Speed: Detect and identify Salmonella species, Typhimurium, and Enteritidis in under 16 hours
  • Simplicity: Simple sample preparation step provides scalability to higher-throughput and sample volumes
  • Accuracy: Applied Biosystems TaqMan-based qPCR technology for sensitivity, specificity and reliability
     

To find out more about the RapidFinder Salmonella Multiplex PCR Workflow and other Thermo Scientific methods for detection of Salmonella in food and associated samples, visit thermofisher.com/salmonella-testing-food

 

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References

  1. Salmonella. (2020). https://www.cdc.gov/salmonella/  
  2. Cost Estimates of Foodborne Illnesses. (2020). https://www.ers.usda.gov/data-products/cost-estimates-of-foodborne-illnesses.aspx