Search Thermo Fisher Scientific
- Order Status
- Quick Order
-
Don't have an account ? Create Account
Search Thermo Fisher Scientific
Microplate Assays for Cell ViabilityHigh-throughput screening that helps provide valuable insights into the overall health and functionality of cells, utilizing metabolic and non-metabolic indicators in fluorescent-based and colorimetric formats |
Microplate assays allow researchers to assess the health and viability of cells quickly and accurately in a high-throughput manner. These assays utilize microplate wells, which can accommodate multiple samples simultaneously, making them exceptional for screening large numbers of compounds or conditions. Microplate assays also provide a valuable means of obtaining information on entire cell populations rather than tracking the behavior of individual cells. This high-throughput screening and analysis can help provide insight into the viability of intact cells, disrupted cells, or cell lysates.
Various cellular functions are utilized in microplate assays to help determine cell viability. Metabolic activity is commonly used as a viability indicator using the reducing power of living cells with alamarBlue and PrestoBlue assays, or by assessing cellular redox potential with MTT and XTT assays. In certain applications, it may be crucial to evaluate viability independently of the metabolic state. For instance, the release of enzymatic proteins can serve as an indicator of viability, which is measured through CyQUANT Cytotoxicity assays. Additionally, the loss of membrane integrity can be assessed using LIVE/DEAD kits.
Thermo Fisher Scientific offers microplate assays with different approaches to assess cell viability that suites each researchers’ specific experimental needs.
The alamarBlue and PrestoBlue Cell Viability Reagents are ready-to-use resazurin-based solution that function as a cell health indicator by using the reducing power of living cells to quantitatively measure viability.
alamarBlue Cell Viability Reagent | alamarBlue HS Cell Viability Reagent | PrestoBlue Cell Viability Reagent | PrestoBlue HS Cell Viability Reagent | |
---|---|---|---|---|
Assay type |
| |||
Use |
|
| ||
Measurement |
|
| ||
Mechanism of detection |
| |||
Assay sensitivity | ![]() | ![]() | ![]() | ![]() |
Cat. No. | DAL1025 | A50100 | A13261 | P50200 |
Learn more about differences in alamarBlue and alamarBlue HS | Learn more about differences in PrestoBlue and PrestoBlue HS |
CyQUANT MTT and CyQUANT XTT Cell Viability Assays are reagents that function as a cell health indicator by using the cellular redox potential of live cells to quantitively measure cell viability and proliferation.
CyQUANT MTT Cell Viability Assay | CyQUANT XTT Cell Viability Assay | |
---|---|---|
Assay type |
| |
Use |
|
|
Measurement |
| |
Mechanism of detection |
|
|
Kit components |
|
|
Assay sensitivity | ![]() | ![]() |
Cat. No. | V13154 | X12223 |
Learn more about differences in CyQUANT XTT and MTT Assays for Cell Viability |
CyQUANT Cytotoxicity Assays use colorimetric- or fluorescent-based detection to measure the release of cytosolic enzymes, LDH or G6PD, from damaged cells.
CyQUANT LDH Cytotoxicity Assay- Fluorescence | CyQUANT Cytotoxicity Assay Kit (G6PD Release Assay) | ||
---|---|---|---|
Assay Type |
|
| |
Use |
|
| |
Measurement |
|
| |
Mechanism of detection |
|
| |
Cat. No. | C20300 | C20302 | V23111 |
Learn more about the differences in CyQUANT Cytotoxicity Assays |
LIVE/DEAD kits can be used on microplate readers to discriminate live from dead cells through the influx of fluorescent dyes.
LIVE/DEAD Viability/Cytotoxicity Kit, for mammalian cells | HCS LIVE/DEAD Green Kit | |
---|---|---|
Assay type |
| |
Use |
|
|
Measurement |
|
|
Detection reagent (Ex./Em.) |
|
*For total cell demarcation, choose from one of these. Both are provided in the kit. |
Cat. No. | L3224 | H10290 |
Learn more about LIVE/DEAD Cell Viability Assays |
LIVE/DEAD kits can be used on microplate readers to detect bacterial or yeast viability through the influx of fluorescent dyes.
LIVE/DEAD BacLight Bacterial Viability Kit | LIVE/DEAD Yeast Viability Kit | |
---|---|---|
Assay type |
|
|
Measurement |
| |
Readout |
|
|
Detection reagent (Ex./Em.) |
|
|
Cat. No. | L7012 | L7009 |
Learn more about LIVE/DEAD Cell Viability Assays |
The alamarBlue and PrestoBlue Cell Viability Reagents are ready-to-use resazurin-based solution that function as a cell health indicator by using the reducing power of living cells to quantitatively measure viability.
alamarBlue Cell Viability Reagent | alamarBlue HS Cell Viability Reagent | PrestoBlue Cell Viability Reagent | PrestoBlue HS Cell Viability Reagent | |
---|---|---|---|---|
Assay type |
| |||
Use |
|
| ||
Measurement |
|
| ||
Mechanism of detection |
| |||
Assay sensitivity | ![]() | ![]() | ![]() | ![]() |
Cat. No. | DAL1025 | A50100 | A13261 | P50200 |
Learn more about differences in alamarBlue and alamarBlue HS | Learn more about differences in PrestoBlue and PrestoBlue HS |
CyQUANT MTT and CyQUANT XTT Cell Viability Assays are reagents that function as a cell health indicator by using the cellular redox potential of live cells to quantitively measure cell viability and proliferation.
CyQUANT MTT Cell Viability Assay | CyQUANT XTT Cell Viability Assay | |
---|---|---|
Assay type |
| |
Use |
|
|
Measurement |
| |
Mechanism of detection |
|
|
Kit components |
|
|
Assay sensitivity | ![]() | ![]() |
Cat. No. | V13154 | X12223 |
Learn more about differences in CyQUANT XTT and MTT Assays for Cell Viability |
CyQUANT Cytotoxicity Assays use colorimetric- or fluorescent-based detection to measure the release of cytosolic enzymes, LDH or G6PD, from damaged cells.
CyQUANT LDH Cytotoxicity Assay- Fluorescence | CyQUANT Cytotoxicity Assay Kit (G6PD Release Assay) | ||
---|---|---|---|
Assay Type |
|
| |
Use |
|
| |
Measurement |
|
| |
Mechanism of detection |
|
| |
Cat. No. | C20300 | C20302 | V23111 |
Learn more about the differences in CyQUANT Cytotoxicity Assays |
LIVE/DEAD kits can be used on microplate readers to discriminate live from dead cells through the influx of fluorescent dyes.
LIVE/DEAD Viability/Cytotoxicity Kit, for mammalian cells | HCS LIVE/DEAD Green Kit | |
---|---|---|
Assay type |
| |
Use |
|
|
Measurement |
|
|
Detection reagent (Ex./Em.) |
|
*For total cell demarcation, choose from one of these. Both are provided in the kit. |
Cat. No. | L3224 | H10290 |
Learn more about LIVE/DEAD Cell Viability Assays |
LIVE/DEAD kits can be used on microplate readers to detect bacterial or yeast viability through the influx of fluorescent dyes.
LIVE/DEAD BacLight Bacterial Viability Kit | LIVE/DEAD Yeast Viability Kit | |
---|---|---|
Assay type |
|
|
Measurement |
| |
Readout |
|
|
Detection reagent (Ex./Em.) |
|
|
Cat. No. | L7012 | L7009 |
Learn more about LIVE/DEAD Cell Viability Assays |
High-sensitivity fluorescence detection for 6-1,536 samples can be quickly performed on the Varioskan ALF or Varioskan LUX Multimode Microplate Reader using Invitrogen reagents to enable optimal detection. Take advantage of automatic dynamic range selection to get optimal gain settings for each individual well and automation capabilities for even higher throughput.
Cell Analysis Learning Center—Find educational resources such as application notes, webinars, videos, articles, and more that cover the use of many of our reagents and kits for cell analysis.
Fluorescence SpectraViewer—Online tool for visualization of the excitation and emission of fluorescent reagents; allows for checking spectral compatibility for multiple fluorophores.
An Absorbance-Based Assay for Cell Health and Proliferation
Cell Analysis Support Center—Find technical information, tips and tricks, and answers to everyday problems.
For Research Use Only. Not for use in diagnostic procedures.