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Instrument calibration
To ensure optimal performance of your thermal cycler or sequence detection system, Applied Biosystems strongly recommends that you regularly calibrate the system. Calibration schedules vary by instrument; refer to the table below for the types of calibration and appropriate intervals.
Instrument calibration intervals
| Calibration Type | Instrument | Interval |
| ROI (Spatial) | 7300/7500/7500 Fast Real-time PCR Systems StepOne™ / StepOnePlus™ Systems | Every 6 monthsorAs needed to verify the instrument’s performance |
| Background | 7900HT Fast Real-Time PCR System 7300/7500/7500 Fast Real-time PCR Systems | Every monthorBefore performing a pure dye calibration orWhen installing an uncalibrated block |
| Pure dye spectra | 7900HT Fast Real-Time PCR System 7300/7500/7500 Fast Real-time PCR Systems | Every 6 months orWhen installing an uncalibrated block |
| Instrument verification | 7900HT Fast Real-Time PCR System 7300/7500/7500 Fast Real-time PCR Systems | Every 6 months orAs needed to verify the instrument’s performance |
For Research Use Only. Not for use in diagnostic procedures.