Quality results using microplastic-free DynaGreen Protein A, Protein A/G and CaptureSelect Anti-IgG-Fc magnetic beads


Thermo Fisher Scientific DynaGreen Protein A, Protein A/G and CaptureSelect Anti-IgG-Fc (Multi-species) magnetic beads are a highly magnetic submicron bead platform with a pioneering greener design, from manufacturing to customer delivery. Building upon Dynal’s strong foundation in biomolecule capture, the team behind Dynabeads magnetic beads has applied more than 30 years of experience with the 12 principles of green chemistry to create high performing, non-microplastic magnetic beads that give reproducible results with low non-specific binding. The choice is a better option for the environment and in line with greener lab policies, without compromising on the quality of results. Leading with DynaGreen Protein A, Protein A/G, and CaptureSelect Anti-IgG-Fc magnetic beads for immunoprecipitation (IP), these are the first products in our growing sustainable magnetic bead lineup.

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Seven ways DynaGreen magnetic beads are contributing to sustainable science

DynaGreen Protein A, Protein A/G, and CaptureSelect Anti-IgG-Fc magnetic beads were produced from a desire and need to move towards more sustainable research laboratories. 12 principles of green chemistry were applied at each development step, leading to a product that’s good for both your work and the environment. Here is how DynaGreen magnetic beads are contributing to more sustainable science:

  • No microplastics—DynaGreen Protein A, Protein A/G, and CaptureSelect Anti-IgG-Fc magnetic beads consist solely of inorganic materials commonly found in nature, including silica and iron oxide.
  • Simplified manufacturing—We have extensively simplified our manufacturing process to reduce manufacturing time and consumption of energy and water.
  • Sustainable chemicals and buffers—DynaGreen Protein A, Protein A/G, and CaptureSelect Anti-IgG-Fc magnetic beads are produced with more sustainable chemicals than other magnetic beads, such as biorenewable solvents, and they can be stored in a buffer that contains a greener and more environmentally friendly surfactant and biocide.
  • Flexibility—No buffers are included with the beads. Buffer recipes that are easy to prepare using common laboratory chemicals are provided in the user guide. This reduces shipping weight and therefore fuel consumption.
  • Paperless documentation—All mandatory documentation is digital (paperless) and accessible with a QR code.
  • Sustainable packaging—DynaGreen Protein A, Protein A/G, and CaptureSelect Anti-IgG-Fc magnetic beads are packaged efficiently with recyclable materials and shipped under ambient conditions. Recycling instructions are provided, and products will not be shipped using plastic or Styrofoam packaging.
  • Transparency—We maintain transparency for sustainability through third-party verification—DynaGreen products are ACT labeled.

High-performance DynaGreen Protein A, Protein A/G, and CaptureSelect Anti-IgG-Fc magnetic beads for immunoprecipitation

We tested DynaGreen Protein A, Protein A/G, and CaptureSelect Anti-IgG-Fc magnetic beads pulldown efficiency against competitor magnetic beads. High-quality pulldown of a high-abundance target protein (CD3) is seen with DynaGreen Protein A magnetic beads compared with magnetic beads from Thermo Fisher Pierce, Promega, Sigma, and Bio-Rad (Figure 1A). DynaGreen Protein A magnetic beads outperformed all competitors in both direct and indirect IP workflows.

Western blot analysis of DynaGreen Protein A/G magnetic beads pulldown of a low-abundance target protein (CD81) shows similar or better pulldown efficiency compared with magnetic beads from Thermo Fisher Pierce, GenScript, Millipore, Sigma, and Bio-Rad (Figure 1B). DynaGreen Protein A/G magnetic beads outperformed all competitors in the indirect IP workflow. Thermo Scientific DynaGreen CaptureSelect Anti-IgG-Fc (Multi-species) beads gave the cleanest IP, while pulling down a comparable amount of target to Thermo Scientific Pierce Protein A/G magnetic beads (indirect IP workflow), Cytiva™ Sera-Mag™ SpeedBeads Protein A/G Magnetic Beads, and Bio-Rad™ SureBeads™ Protein G Magnetic Beads (direct IP workflow).

A

Western blot of DynaGreen Protein A Magnetic Beads vs five competitor beads at immunoprecipitating protein CD3

B

Western blot of DynaGreen Protein A/G Magnetic Beads vs ten competitor beads at immunoprecipitating protein CD81

Figure 1. Comparable or better performance with DynaGreen magnetic beads. (A) DynaGreen Protein A magnetic beads compared to five other competitor beads at indirect and direct IP of a high-abundance protein target, CD3. Lane 1: Promega Magnet Protein A (Promega), lane 2: DynaGreen Protein A (Thermo Fisher), lane 3: Protein Xtra Sepharose Protein A (Cytiva – Sigma), lane 4: Pierce Protein A (Thermo Fisher), lane 5: Dynabeads Protein A (Thermo Fisher), lane 6: DynaGreen Protein A (Thermo Fisher), lane 7: Blank/no sample, lane 8: Protein A Magnetic Sepharose (Cytiva – Sigma), and lane 9: SureBeads Protein A (BioRad). (B) DynaGreen Protein A/G magnetic beads compared to ten other competitor beads at indirect and direct IP of a low-abundance protein target, CD81. Lane 1: SeraMag Speed bead protein A/G (Sigma), lane 2: CaptureSelect IgG-Fc (ms) Magnetic Agarose Beads (Thermo Fisher), lane 3: Protein Xtra Sepharose, Protein G (Cytiva – Sigma), lane 4: Pierce Protein G (Thermo Fisher), lane 5: Pierce Protein A/G Magnetic Beads (Thermo Fisher), lane 6: DynaGreen Anti IgG-Fc (Thermo Fisher), lane 7: DynaGreen Protein A/G (Thermo Fisher), lane 8: GenScript Protein A/G Mag beads (GenScript), lane 9: Dynabeads Protein G (Thermo Fisher), lane 10: DynaGreen Protein A/G (Thermo Fisher), lane 11: DynaGreen Anti IgG-Fc (Thermo Fisher), lane 12: PureProteome Protein A/G Magnetic System (Millipore), lane 13: PureProteome Protein G Magnetic System (Millipore), lane 14: Protein G Magnetic Sepharose (Cytiva – Sigma), and lane 15: SureBeads Protein G (BioRad). Equal amounts of sample material with respect to Ab and cell lysate were used for all IP protocols according to the manufacturer’s protocols.


DynaGreen Protein A, Protein A/G, and Anti-IgG-Fc magnetic beads selection guide

 

Image showing a vial of DynaGreen Protein A magnetic beads and the secondary cardboard packaging
DynaGreen Protein A Magnetic Beads

Image showing a vial of DynaGreen Protein A/G magnetic beads and the secondary cardboard packaging
DynaGreen Protein A/G Magnetic Beads

DynaGreen CaptureSelect Anti-IgG-Fc
DynaGreen CaptureSelect Anti-IgG-Fc Magnetic Beads

When to use
  • When using rabbit antibody, particularly to pull down large amounts of protein
  • When using mouse and rabbit antibodies, particularly when you want high yields of target protein
  • Suitable for all types of uses and is highly compatible with mass spectrometry
  • May be used with secondary antibodies from multiple species
  • When a high purity of sample is required, particularly for applications such as mass spectrometry
Binding capacity13–14 µg/mL
Size260–280 µg/mL
Concentration20 mg/mL
Quantity beads per IP25 µl*
YieldHighHighMedium
Non-specific bindingLowLowUltra-low
Experiment protocolsIP (direct and indirect), mass spectrometry
AutomationAutomation ready—protocols available for KingFisher Purification Systems
Recyclable primary packagingYes—recycling instructions provided digitally with pack insert
SustainabilityACT label third-party verification of the sustainable impacts of a product, its manufacturing operations, and its end of life

* High binding capacity means that a smaller amount of beads are required per IP. Competitors normally use 40–100 µl.



How to use DynaGreen magnetic beads in your lab

Immunoprecipitation with DynaGreen Protein A, Protein A/G, and CaptureSelect Anti-IgG-Fc magnetic beads

IP is the affinity purification of proteins, protein complexes, protein-nucleic acid complexes, and other antigens (Ag) using a specific antibody that is immobilized to a solid support such as magnetic beads. DynaGreen magnetic beads are 250-micron superparamagnetic beads that enable high performance direct and indirect IP. The sub-micron bead size of DynaGreen magnetic beads increases the available target capture surface area and provides a low sedimentation rate resulting in efficient isolation of target protein in a simple bind-incubate-elute immunoprecipitation protocol that does not require pre-clearing. The magnetic separation technology used is also rapid and gentle, thereby causing minimal physical stress to your target proteins.

Additional benefits of DynaGreen Protein A, Protein A/G, and CaptureSelect Anti-IgG-Fc magnetic beads include:

  • Reproducibility, high yield, and sample purity after direct and indirect IP protocols (Figure 2)
  • Adaptable protocols for downstream IP assays such as western blot and mass spectrometry
  • Sustainable and holistic product design with energy efficient manufacturing, recyclable packaging, and non-microplastic magnetic bead core
  • ACT labeled—third-party verification of environmental impacts
  • Backed by 30 years of Dynabeads quality and innovation
  • Automation compatible to Thermo Scientific KingFisher systems—40 mins from the press of a button to finished sample preparation

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Diagram illustrating the steps of direct immunoprecipitation using DynaGreen Protein A Magnetic Beads and Protein A/G Magnetic Beads: 1) antibody is added to DynaGreen magnetic beads for 30 mins, 2) sample is added to mix for 30 mins, 3) wash steps, and 4) elution
Diagram illustrating the steps of indirect immunoprecipitation using DynaGreen Protein A Magnetic Beads and Protein A/G Magnetic Beads: 1) antibody is added to sample for 30 mins, 2) DynaGreen magnetic beads is added to mix for 30 mins, 3) wash steps, and 4) elution

Figure 2. DynaGreen Protein A, Protein A/G, and CaptureSelect Anti-IgG-Fc magnetic beads for immunoprecipitation (IP). (A) Direct IP begins by 1) adding your choice of antibody to DynaGreen magnetic beads for 30 mins, 2) add sample to the antibody-bead mix for 30 mins, 3) washing of the beads to remove unbound, non-specific binding, and 4) elute target proteins from the beads with elution buffer. (B) Indirect IP begins by 1) adding your choice of antibody to your sample for 30 mins, 2) add DynaGreen magnetic beads to the sample-antibody mix for 30 mins, 3) washing of the beads to remove unbound, non-specific binding, and 4) elute target proteins from the beads with elution buffer.


How to elute Protein A, Protein A/G, and CaptureSelect Anti-IgG-Fc magnetic beads

After incubation of the beads-ligand-antibody complex and washing steps, elution is the last step of both direct and indirect IP. After sufficient incubation time of the antibody, magnetic beads, and sample, the Protein A, Protein A/G, and CaptureSelect Anti-IgG-Fc magnetic beads are washed and elution buffer is added to the beads to unbind the target protein from the magnetic beads, leaving you with your target protein ready for downstream analysis such as western blot or mass spectrometry (Figure 2).

Mass spectrometry workflow with DynaGreen magnetic beads

Mass spectrometry is a powerful tool for identification, quantification, and characterization of proteins in complex biological samples. A standard proteomics workflow includes sample extraction, protein isolation and digestion, HPLC separation, and analysis of the resulting peptides with tandem mass spectrometry. DynaGreen Protein A, Protein A/G, and CaptureSelect Anti IgG-Fc magnetic beads have been validated for successfully isolating antibody-protein complexes for use in downstream mass spectrometry analysis. The protocol for a suggested mass spec workflow can be viewed below:

  • Tissue
  • Cell culture
  • Serum
  • Plasma
  • Biofluids
  • Yeast
  • Tissue
  • Cell culture
  • Serum
  • Plasma
  • Biofluids
  • Yeast


DynaGreen magnetic beads ordering information

For Research Use Only. Not for use in diagnostic procedures.

© 2023 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
ACT is a trademark of My Green Lab.
Magne is a trademark of Promega Corporation.
Sepharose is a trademark of Cytiva Bioprocess R&D AB.
SureBeads is a trademark of Bio-Rad Laboratories, Inc.
Sera-Mag is a trademark of Hyclone Laboratories, LLC.
GenScript is a trademark of Nanjing GenScript Biotech Co.
PureProteome is a trademark of Millipore Corporation.

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