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Cell type | Antigen | Type | ICC dilution* |
---|---|---|---|
Neural stem cells | Sox1 | Goat IgG | 10 μg/mL |
Sox2 | Mouse IgG | 2 μg/mL | |
Nestin | Mouse IgG | 1:500 | |
CD133 | Rabbit IgG | 1:100 | |
Neuronal progenitors Neurons (Pan) | MAP2 | Mouse IgG | 1:200 |
HuC/Dl | Mouse IgG | 10 μg/mL | |
NF | Mouse IgG | 1:100 | |
NCAM | Mouse IgG | 1:50 | |
βIII tubulin | Mouse IgG | 1:2,000 | |
Dcx | Rabbit IgG | 1:200 | |
Dopaminergic neurons | TH | Rabbit IgG | 1:1,000 |
Motoneurons | Isl1 | Mouse IgG | 1:50 |
HB9 | Mouse IgG | 1:50 | |
GABAergic/Glutaminergic neurons | GABA | Rabbit IgG | 1:2,000 |
Cholinergic neurons | ChAT | Goat IgG | 1:100 |
Astrocyte progenitors Astrocytes | CD44 | Mouse IgG | 1:50 |
GFAP | Rat IgG | 1:100 | |
GFAP | Rabbit IgG | 1:200 | |
Oligodendrocyte progenitors Oligodendrocytes | GalC | Mouse IgG | 1:200 |
NG2 | Mouse IgG | 1:200 | |
A2B5 | Mouse IgM | 1:2,000 | |
O4 | Mouse IgM | 1:50 | |
Proliferation | Ki67 | Rabbit IgG | 1:50 |
EdU | Chemicals | 1:1,000 | |
Isotype control | Mouse | IgM and IgG | Use as is |
Rabbit | IgG | Use as is |