Cytokines and Growth Factors for Organoid Culture

Organoid culture is a cutting-edge technique that allows researchers to recreate miniature organs in a controlled laboratory environment. This innovative approach offers numerous advantages, including the ability to study organ development, disease progression, and drug efficacy in a highly accurate and reproducible manner. To achieve a successful organoid model, it is crucial to utilize consistent culture components. Regardless of the specific cell type or research objective, the use of high-quality cytokines and growth factors is essential to ensure reproducibility and maintain consistency throughout the organoid culture process.

Thermo Fisher Scientific manufactures Peprotech recombinant cytokines and growth factors to support your organoid culture and organoid/tumoroid model development.

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Figure 1. Human FGF-4 recombinant protein and WNT3A act in synergy to differentiate human episomal iPSCs into mid- and hindgut endoderm and promote a gut tube-like morphogenesis, resulting in the formation of 3D mid- and hindgut-like spheroids. The major components present in the differentiation media are human Noggin recombinant protein and EGF recombinant human protein. Panels A and B represent the merged fluorescence images of SOX9 recombinant rabbit monoclonal antibody (7H13L8) (green) with DAPI (blue) and F-actin (red) staining for iPSC control cells (A) and intestinal organoid (B). The images were captured at 60X magnification.

Figure 2. Differentiation of human episomal iPSCs (A) into kidney organoid (B) is initiated with human Noggin recombinant protein and a GSK-3 inhibitor. Cells are later treated with human Activin A recombinant protein and human FGF-9 recombinant protein to induce nephron progenitor cells. Panels A and B represent the merged fluorescence images of PODXL monoclonal antibody 3D3 (green) with DAPI (blue) and F-actin (red) staining for iPSC control cells and differentiated kidney organoid. The images were captured at 60X magnification.