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Use our oligo calculators to determine volumes needed to resuspend your DNA oligos to desired concentrations, estimate the percentage of full-length product for different oligo lengths, and calculate final yield based on oligo length, synthesis scale, and purification method.
This resuspension calculator calculates the volume needed to resuspend an oligo, given that nmol and desired concentration are known.
| Term | Number |
|---|---|
| Oligo yield (nmol, from COA) | |
| Desired concentration (µM or µmol/L) | |
| Volume required (µL) | ... |
This calculates the estimated percentage of full-length product based on the oligo's length.
| Term | Number |
|---|---|
| Oligo length (nt) | |
| Chem. reaction efficiency (typically 98–99% efficient) | 99% |
| % Full length product | ... |
This calculates the minimum yield guaranteed* based on the oligo length (nt) and synthesis scale.
Enter your oligo length to populate the yields below.
| Term | Number |
|---|---|
| Oligo length (nt) | |
| Approx. mol. weight (daltons or g/mol) | ... |
| pmol in 1 µg of primer | ... |
The amounts calculated in the table below represent the minimum guaranteed amount of oligo you will receive based on the oligo length (nt), synthesis scale, and purification method.
| Synthesis scales | Desalted (nmol) | Cartridge (nmol) | HPLC (nmol) | PAGE (nmol) |
|---|---|---|---|---|
| 25 nmol | N/A | N/A | N/A | |
| 50 nmol | ||||
| 200 nmol | ||||
| 1,000 nmol (1 µmol) | ||||
| 10,000 nmol (10 µmol) | N/A |
The volumes calculated in the table below represent the minimum guaranteed amount of oligo you will receive based on the oligo length (nt), synthesis scale, purification method, and selected concentration.
| Selected conc. | Synthesis scales | Desalted (µL) | Cartridge (µL) | HPLC (µL) | PAGE (µL) |
|---|---|---|---|---|---|
| 10 µM | 25 nmol | N/A | N/A | N/A | |
| 50 nmol | |||||
| 200 nmol | |||||
| 1 µmol | |||||
| 10 µmol | N/A | ||||
| 20 µM | 25 nmol | N/A | N/A | N/A | |
| 50 nmol | |||||
| 200 nmol | |||||
| 1 µmol | |||||
| 10 µmol | N/A | ||||
| 50 µM | 25 nmol | N/A | N/A | N/A | |
| 50 nmol | |||||
| 200 nmol | |||||
| 1 µmol | |||||
| 10 µmol | N/A | ||||
| 100 µM | 25 nmol | N/A | N/A | N/A | |
| 50 nmol | |||||
| 200 nmol | |||||
| 1 µmol | |||||
| 10 µmol | N/A | ||||
| 200 µM | 25 nmol | N/A | N/A | N/A | |
| 50 nmol | |||||
| 200 nmol | |||||
| 1 µmol | |||||
| 10 µmol | NA |
* Minimum yields guaranteed. When ordering custom oligos, remember that the scale of synthesis (25 nmol, 50 nmol, 200 nmol, 1 µmol, or 10 µmol) is the starting point for synthesis, not the guaranteed final amount. Oligonucleotide length, sequence, GC content, and coupling efficiencies can vary the yield of nmol amounts post-synthesis. Thermo Fisher Scientific guarantees the total yield of oligonucleotide as a minimum number of nmol. Purification by HPLC, PAGE, and cartridge will also affect final product yield.
For Research Use Only. Not for use in diagnostic procedures.