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The following checklist provides a concise list of suggestions and procedures to guide you to achieve a solid aseptic technique. For an in-depth review of aseptic technique, refer to Culture of Animal Cells: A Manual of Basic Technique (Freshney, 2000).
Question | Completed | |
---|---|---|
Work Area | ||
Is the cell culture hood properly set up? | ||
Is the cell culture hood in an area free from drafts and through traffic? | ||
Is the work surface uncluttered, and does it contain only items required for your experiment? | ||
Did you wipe the work surface with 70% ethanol before work? | ||
Are you routinely cleaning and sterilizing your incubators, refrigerators, freezers, and other laboratory equipment? | ||
Personal Hygiene | ||
Did you wash your hands? | ||
Are you wearing personal protective equipment? | ||
If you have long hair, is it tied in the back? | ||
Are you using a pipettor to work with liquids? | ||
Reagents & Media | ||
Have you sterilized any reagents, media, and solutions you have prepared in the laboratory using the appropriate procedure? | ||
Did you wipe the outside of the bottles, flasks, and plates with 70% ethanol before placing them on your work surface? | ||
Are all your bottles, flasks, and other containers capped when not in use? | ||
Are all your plates stored in sterile re-sealable bags? | ||
Does any of your reagents look cloudy? Contaminated? Do they contain floating particles? | ||
Do you detect a foul smell? Unusual color? If yes, did you decontaminate and discard those items? | ||
Handling | ||
Are you working slowly and deliberately, mindful of aseptic technique? | ||
Did you wipe the surfaces of all the items including pipettor, bottles, and flasks with 70% ethanol before placing them in the cell culture hood? | ||
Are you placing the caps or covers face down on the work area? | ||
Are you using sterile glass pipettes or sterile disposable plastic pipettes to manipulate all liquids? | ||
Are you using a sterile pipette only once to avoid cross contamination? | ||
Are you careful not to touch the pipette tip to anything non-sterile, including the outside edge of the bottle threads? | ||
Did you mop up any spillage immediately, and wiped the area with 70% ethanol? |