Calculation worksheets for LanthaScreen® Eu Kinase Binding Assay

3X Tracer (~3X Kd):

Tracer: Stock = nM
1X tracer = nM
3X tracer = nM

To prepare μL ____ :

Buffer: _____ μL – _____ μL = _____ μL 1X Kinase Buffer A

Add the calculated volume of tracer to the calculated volume of 1X Kinase Buffer A.

Note: Tracer Kd values for Invitrogen™ kinases are shown in the detailed protocol


3X Kinase/3X Antibody:

Kinase:   _____  Molecular weight = _____   kDa (from kinase COA) = μg/nmol

_____mg         x   1,000 μg    x    1 nmol    =    ______ nmol
            mL                1 mg                ___μg                       mL


____  nmol         x   1,000 ml    x   ___nmol    =    ______ nM
            mL                   1 L                          L                   


Kinase stock = ____ nM
1X = 5 nM
3X = 15 nM

Antibody stock =  ____ μM = ____ nM
1X = 2 nM
3X = 6 nM


Note: If V1 is less than 2 μL, perform an intermediate 1:10 dilution. We suggest taking 2.0 μL of kinase and adding it to 18 μL of 1X Kinase Buffer A to prepare 20 μL of diluted kinase

Add the calculated volume of tracer to the calculated volume of 1X Kinase Buffer A.

To prepare 1,500 μL:

Buffer:  1,500 ____ μL – ____ μL – ____ μL = ____ μL 1X Kinase Buffer A

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